The research of my group is focused on the structure and function of biological membranes. We are using a variety of biophysical (fluorescence spectroscopy, computer simulations), biochemical (protein purification, protein labeling, kinetics, cell biology), molecular biological (cloning and mutagenesis), and chemical (peptide and lipid synthesis) methods to answer the following questions:
- How do lipids and proteins interact in a membrane?
- How do lipids affect the activity of the Ca-ATPase of skeletal muscle sarcoplasmic reticulum?
- How do lipids affect the function of channels such as the KcsA potassium channel and the mechanosensitive channel MscL?
- How do lipids affect the function of membrane-bound enzymes such as the bacterial diacylglycerol kinase?
- How do simple transmembrane a-helices as found in the Ca-ATPase, diacylglycerol kinase and KcsA interact with lipid bilayers?
- How do proteins interact in a lipid bilayer?
- How do phospholamban and sarcolipin interact with the Ca-ATPase and control its activity?
- How do simple transmembrane a-helices interact and form helical bundles in a membrane?
- How do transport proteins such as the Ca-ATPase transport ions across the membrane?
- How is the hydrolysis of ATP coupled to movement of Ca?
- How are membrane proteins targeted to the correct membrane in a cell?
Molecular and Cellular Biosciences
Emeritus Professor Anthony Lee
Biological Sciences Faculty of Natural & Environmental Sciences Life Sciences Building 85 University of Southampton Highfield Campus Southampton SO17 1BJ
Telephone:(023) 8059 4331