Molecular Tools for Marine Biology Research

Learning Outcomes

Learning Outcomes

Having successfully completed this module you will be able to:

• Use molecular basis to interpret population and ecosystem-wide level processes.
• Distinguish between, and use, a range of library information and bioinformatic database services.
• Explain the principles and application of a range of molecular biological experimental research techniques to biological oceanographic studies.
• Describe basic aspects of cellular processes in marine organisms, with particular focus on gene transcription, translation, gene structure and expression, gene regulation in response to environmental parameters and bioluminescence.
• Apply a range of basic molecular biological and biochemical methods in silico and in the laboratory.
• Analyse laboratory data appropriately, organise the results from experiments and report them effectively to a specific deadline.

Transferable and Generic Skills

Having successfully completed this module you will be able to:

• Time management
• Individual report writing;
• Small-team working;

Subject Specific Intellectual and Research Skills

Having successfully completed this module you will be able to:

• Bioinformatic data base work and analysis of retrieved data
• Technical laboratory skills in aspects of molecular biology and the manipulation of nucleic acids and proteins
• Library information retrieval and critical analysis of relevant literature
• Interrogation, analysis and presentation of experimental data

Molecular biological methods have revolutionised life science research over the last decades. As basic functions are ubiquitous in living cells, molecular biological methods offer unprecedented potential to answer questions in the marine realm, ranging from the function of single enzymes to global-scale implications of material cycles.

Knowledge about the molecular physiology of cells is not only important for an integral interpretation of the physiology of marine organisms, but is also the prerequisite to understand the molecular biological methods taught in the lectures and practicals in the later stage of the unit. In this part, the students will become familiar with an array of widely used research methods that allow the analysis of nucleic acids and proteins. The methods will be explained as part of marine biological case studies that provide examples for their applications and highlight the importance of molecular biological experimentation.

The practical sessions include the extraction and purification of nucleic acids and proteins from marine organisms and their analysis including chromatographic and spectroscopic methods and test of their function. In addition, the students will gather practical experience in the use of computers for molecular biological analysis and data base exploration.

Teaching and learning methods

Formal Lectures: Lectures will provide the fundamental theory and principles of the module. Each lecture systematically covers the main concepts and topics by the use of PowerPoint presentations which are supported by illustrated handout materials and Blackboard and Panopto. Where relevant, lecturer's own research experience is brought into the lecture sessions. References to relevant journal articles are provided as essential reading for lectures.

Interactive Learning Sessions: The lectures providing theoretical background are paired back-to-back with in-class practicals (interactive learning sessions) during which the students work alone or in groups to deepen their learning by putting the theoretical concepts into practise through data analysis, date base work, solving research tasks using online bioinformatic tools, and the analysis of research papers.

Laboratory Practicals: Each student is required to attend experimental classes to acquire appropriate skills in molecular biology, specifically the biochemistry of nucleic acids (students will work alone or in small groups). Each practical is supported by, and integrated with, formal lectures together with an introductory talk, reflecting the lecturer's research expertise. The practical sequence is supported by a laboratory report on the theoretical background, experimental results and health & safety aspects of the relevant practicals.

Support: Lectures are Panopto-recorded if technically possible. Synopses of lectures and practicals are posted on Blackboard. In laboratory practicals, support is provided by staff and postgraduate demonstrators.

A wide range of support can be provided for those students who have further or specific learning and teaching needs.

Summative

This is how we’ll formally assess what you have learned in this module.

Repeat Information

Repeat type: Internal & External