Synthetic biochemistry of the microtubule cytoskeleton: Mechanistic insight from reverse engineering Event

Abstract: The cytoskeleton is essential for the internal organization of eukaryotic cells. Microtubules, motors and other proteins form a mechano-chemical network that determines the dynamic and adaptable nature of intracellular order. Distinct forms of cytoskeletal architectures are required for correct cell division and cell differentiation. How the components of the microtubule cytoskeleton work together as a system is not understood. We address these questions combining concepts of physical biochemistry and quantitative cell biology. Our aim is to understand in quantitative terms the molecular mechanisms underlying cytoskeleton architecture and function. Here, I will present recent progress in understanding molecular mechanisms of cytoskeleton function through reverse engineering of dynamic behavior. The focus will be on the regulation of microtubule dynamics and microtubule nucleation. The power of in vitro reconstitutions in combination with fluorescence microscopy will be illustrated in the context of regulatory proteins binding preferentially to dynamic microtubule ends.