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The University of Southampton
Biomedical Imaging Unit

Publications from the BIU

Being a core service facility, BIU staff rarely have time to undertake their own research. However, we do make significant contributions to many ongoing research projects, resulting in co-authorship of numerous scientific papers each year. Recent publications are shown below with BIU staff shown in bold . We also provide advice, support and imaging services to many other projects, resulting in the BIU being acknowledged in the publications from those projects.

We endorse both the Royal Microscopical Society's Core Facilities Publication Policy concerning the crediting of imaging facility staff for their contribution to published works, and the University of Southampton's Fair Attribution Guidance.

If you do publish work that includes data from our instruments, please do acknowledge the BIU in your paper and send us a copy for our records.

BIU Publications 2024

Panchal MH, Swindle EJ, Pell TJ, Rowan WC, Childs CE, Thompson J, Nicholas BL, Djukanovic R, Goss VM, Postle AD, Davies DE, Blume C (2024) Membrane lipid composition of bronchial epithelial cells influences antiviral responses during rhinovirus infection. Tissue Barriers: 2300580. doi: 10.1080/21688370.2023.2300580. Epub ahead of print. PMID: 38179897.

Pearson-Farr JE, Doherty R, Chatelet DS, Goggin P, Ng KYB, Lucas JS, Cleal JK, Cheong YC, Lewis RM (2024) Ultrastructural cilia defects in multi-ciliated uterine glandular epithelial cells from women with reproductive failure. Reproduction; 167(1):e230173. doi: 10.1530/REP-23-0173. PMID: 37999981.

Digpal R, Arkill KP, Doherty R, Yates J, Milne LK, Broomes N, Katsamenis OL, Macdonald J, Ditchfield A, Narata AP, Darekar A, Carare RO, Fabian M, Galea I, Bulters D (2024) A Systematic Review and Meta-Analysis of the Pathology Underlying Aneurysm Enhancement on Vessel Wall Imaging. Int J Mol Sci; 25(5):2700. doi: 10.3390/ijms25052700. PMID: 38473947.

Laundon D, Proudley E, Basford PJ, Katsamenis OL, Chatelet DS, Cleal JK, Gostling NJ, Chavatte-Palmer P, Lewis RM. 2024. Quantitative microCT imaging of a whole equine placenta and its blood vessel network. Placenta, 154: 216-219.

Hartnell IJ, Woodhouse D, Jasper W, Mason L, Marwaha P, Graffeuil M, Lau LC, Norman JL, Chatelet DS, Buee L, Nicoll JAR, Blum D, Dorothee G, Boche D 2024 Glial reactivity and T cell infiltration in frontotemporal lobar degeneration with tau pathology. Brain; 147(2):590-606. doi: 10.1093/brain/awad309. PMID: 37703311.

Vom Scheidt A, Krug J, Goggin P, Bakker AD, Busse B (2024) 2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS). Curr Osteoporos Rep; 22(4):396-415. doi: 10.1007/s11914-024-00877-z. Epub 2024 Jul 9. PMID: 38980532; PMCID: PMC11324773.

Laundon D, Sengers BG, Thompson J, Harris SE, Beasley O, Basford PJ, Katsamenis OL, Goggin P, Derisoud E, Fanelli D, Bocci C, Camillo F, Shotton J, Constable-Dakeyne G, Gostling NJ, Chavatte-Palmer P, Lewis RM (2024) Convergently evolved placental villi show multiscale structural adaptations to differential placental invasiveness. Biol Lett; 20(3):20240016. doi: 10.1098/rsbl.2024.0016. Epub 2024 Mar 27. PMID: 38531417.

Morone N, Martin MG, Goggin P, Czymmek KJ, Mennella V, Gonzalez JL (2024) A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium. Methods Mol Biol. 2024;2725:121-129. doi: 10.1007/978-1-0716-3507-0_7. PMID: 37856021.

Angus RB, Maté JF, Angus EM, Král D (2024) Towards a revision of the Palaearctic species of Aphodius Hellwig, 1798, subgenus Liothorax Motschulsky, 1860 (Coleoptera, Scarabaeidae, Aphodiinae). Zookeys. 2024 Jul 22;1207:205-299. doi: 10.3897/zookeys.1207.117225. eCollection 2024. PMID: 39081542

Schreck LD, Pedersen ESL, Dexter K, Manion M; Living with PCD Study Advisory Group; Massin N, Maitre B, Goutaki M, Kuehni CE (2024) Infertility and pregnancy outcomes among adults with primary ciliary dyskinesia. Hum Reprod Open; 2024(3):hoae039. doi: 10.1093/hropen/hoae039. eCollection 2024. PMID: 38962571.

Bell JA, Davies ER, Brereton CJ, Vukmirovic M, Roberts JJW, Lunn K, Wickens L, Conforti F, Ridley RA, Ceccato J, Sayer LN, Johnston DA, Vallejo AF, Alzetani A, Jogai S, Marshall BG, Fabre A, Richeldi L, Monk PD, Skipp P, Kaminski N, Offer E, Wang Y, Davies DE, Jones MG (2024) Spatial transcriptomic validation of a biomimetic model of fibrosis enables re-evaluation of a therapeutic antibody targeting LOXL2. Cell Rep Med; 101695. doi: 10.1016/j.xcrm.2024.101695. Epub ahead of print. PMID: 39173635.

BIU Publications 2023

Rumman N, Fassad MR, Driessens C, Goggin P, Abdelrahman N, Adwan A, Albakri M, Chopra J, Doherty R, Fashho B, Freke GM, Hasaballah A, Jackson CL, Mohamed MA, Abu Nema R, Patel MP, Pengelly RJ, Qaaqour A, Rubbo B, Thomas NS, Thompson J, Walker WT, Wheway G, Mitchison HM, Lucas JS (2023) The Palestinian primary ciliary dyskinesia population: first results of the diagnostic and genetic spectrum. ERJ Open Res; 9(2):00714-2022. doi: 10.1183/23120541.00714-2022. PMID: 37077557.

Noorani I, Sidlauskas K, Pellow S, Savage R, Norman JL, Chatelet DS, Fabian M, Grundy P, Ching J, Nicoll JAR, Boche D (2023). Clinical impact of anti-inflammatory microglia and macrophage phenotypes at glioblastoma margins. Brain Commun; 5(3):fcad176. doi: 10.1093/braincomms/fcad176. PMID: 37324244.

Laundon D, Katsamenis OL, Thompson J, Goggin P, Chatelet DS, Chavatte-Palmer P, Gostling NJ, Lewis RM (2023) Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue. Methods Cell Biol;177:241-267. doi: 10.1016/bs.mcb.2023.01.014. Epub 2023 Apr 10. PMID: 37451769.

Devlin LA, Coles J, Jackson CL, Barroso-Gil M, Green B, Walker WT, Thomas NS, Thompson J, Rock SA, Neatu R, Powell L, Molinari E; Genomics England Research Consortium; Wilson IJ, Cordell HJ, Olinger E, Miles CG, Sayer JA, Wheway G, Lucas JS (2023) Biallelic variants in CEP164 cause a motile ciliopathy-like syndrome. Clin Genet;103(3):330-334. doi: 10.1111/cge.14251. Epub 2022 Nov 3. PMID: 36273371.

Khasati A, Thaung C, Mudhar HS, Wagner B, Goggin P, Stoker I, Sagoo MS, Damato B, Quhill H (2023). Subconjunctival Ocular Argyrosis following Treatment with Ruthenium 106 Brachytherapy for Choroidal Melanoma. Ocul Oncol Pathol; 9(3-4):101-106. doi: 10.1159/000531385. Epub 2023 Jun 5. PMID: 38046271; PMCID: PMC10689982.

Katsamenis OL, Basford PJ, Robinson SK, Boardman RP, Konstantinopoulou E, Lackie PM, Page A, Ratnayaka JA, Goggin PM, Thomas GJ, Cox SJ, Sinclair I, Schneider P (2023) A high-throughput 3D X-ray histology facility for biomedical research and preclinical applications. Wellcome Open Res; 8:366. doi: 10.12688/wellcomeopenres.19666.1. PMID: 37928208; PMCID: PMC10620852.

Fassad MR, Rumman N, Junger K, Patel MP, Thompson J, Goggin P, Ueffing M, Beyer T, Boldt K, Lucas JS, Mitchison HM (2023). Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations. Hum Mol Genet; 32(21):3090-3104. doi: 10.1093/hmg/ddad132. PMID: 37555648; PMCID: PMC10586200.

Kelly L, Sharp MM, Thomas I, Brown C, Schrag M, Antunes LV, Solopova E, Martinez-Gonzalez J, Rodríguez C, Carare RO (2023). Targeting lysyl-oxidase (LOX) may facilitate intramural periarterial drainage for the treatment of Alzheimer's disease. Cereb Circ Cogn Behav 23;5:100171. doi: 10.1016/j.cccb.2023.100171. PMID: 37457664.

Garland EF, Dennett O, Lau LC, Chatelet DS, Bottlaender M, Nicoll JAR, Boche D (2023).The mitochondrial protein TSPO in Alzheimer's disease: relation to the severity of AD pathology and the neuroinflammatory environment. J Neuroinflammation 20(1):186. doi: 10.1186/s12974-023-02869-9. PMID: 37580767.

Pascoe D., Grant I, Johnston D (2023) The Warship Hazardous Prize – Site Investigations 1992–2022. International Journal of Nautical Archaeology, 53(1), 74–102. https://doi.org/10.1080/10572414.2023.2255294

BIU Publications 2022

Eyres M, Bell JA, Davies ER, Fabre A, Alzetani A, Jogai S, Marshall BG, Johnston DA, Xu Z, Fletcher SV, Wang Y, Marshall G, Davies DE, Offer E, Jones MG (2022). Spatially resolved deconvolution of the fibrotic niche in lung fibrosis. Cell Rep;40(7):111230. doi: 10.1016/j.celrep.2022.111230. PMID: 35977489; PMCID: PMC10073410.

Chachlioutaki K, Karavasili C, Adamoudi E, Tsitsos A, Economou V, Beltes C, Bouropoulos N, Katsamenis OL, Doherty R, Bakopoulou A, Fatouros DG (2022) Electrospun Nanofiber Films Suppress Inflammation In Vitro and Eradicate Endodontic Bacterial Infection in an E. faecalis-Infected Ex Vivo Human Tooth Culture Model. ACS Biomater Sci Eng. 8(5):2096-2110. doi: 10.1021/acsbiomaterials.2c00150. Epub 2022 Apr 15. PMID: 35427110.

Lewis RM, Baskaran H, Green J, Tashev S, Palaiologou E, Lofthouse EM, Cleal JK, Page A, Chatelet DS, Goggin P, Sengers BG (2022) 3D visualization of trans-syncytial nanopores provides a pathway for paracellular diffusion across the human placental syncytiotrophoblast. iScience. 25(12):105453. doi: 10.1016/j.isci.2022.105453. PMID: 36387021; PMCID: PMC9663330.

Davies R, Griffiths C, Askelund K, Palaiologou E, Cleal JK, Page A, Chatelet DS, Goggin P, Chamley L, Lewis RM (2022) Microvillous tip vesicles may be an origin of placental extracellular vesicles. Placenta; 123:24-30. doi: 10.1016/j.placenta.2022.04.003. Epub 2022 Apr 20. PMID: 35533511.

Tashev SA, Parsons D, Hillman C, Harris S, Lofthouse EM, Goggin P, Chatelet DS, Cleal JK, Smyth N, Palaiologou H, Page A, Lewis RM (2022) Folding of the syncytiotrophoblast basal plasma membrane increases the surface area available for exchange in human placenta. Placenta;117:57-63. doi: 10.1016/j.placenta.2021.11.002. Epub 2021 Nov 4. PMID: 34768170.

Pearson-Farr J, Wheway G, Jongen MS, Goggin P, Lewis R, Cheong Y, Cleal J (2022) Endometrial gland specific progestagen-associated endometrial protein and cilia gene splicing changes in recurrent pregnancy loss. Reprod Fertil; 3(3):162–72. doi: 10.1530/RAF-22-0002. Epub ahead of print. PMID: 35971960; PMCID: PMC9513660.

Falcone S, Nicol T, Blease A, Randles MJ, Angus E, Page A, Tam FWK, Pusey CD, Lennon R, Potter PK (2022) A novel model of nephrotic syndrome results from a point mutation in Lama5 and is modified by genetic background.Kidney Int; 101(3):527-540. doi: 10.1016/j.kint.2021.10.031. Epub 2021 Nov 10. PMID: 34774562.

Keeling E, Lynn SA, Koh YM , Scott JA, Kendall A, Gatherer M, Page A, Cagampang FR, Lotery AJ, Ratnayaka JA (2022) A High Fat "Western-style" Diet Induces AMD-like Features in Wildtype Mice. Mol Nutr Food Res 66(11): e2100823. doi: 10.1002/mnfr.202100823.

Dexter K, Foster J, Sosabowski J, Milos Petrik M (2022). Preclinical PET and SPECT Instrumentation. Nuclear Medicine and Molecular Imaging, 2022, p. 473-484. Doi: 10.1016/b978-0-12-822960-6.00055-7.

Prasannan P, Siney E, Chatterjee S, Johnston D, Shah M, Mudher A, Willaime-Morawek S. (2022). A 3D-induced pluripotent stem cell-derived human neural culture model to study certain molecular and biochemical aspects of Alzheimer’s disease. In vitro models 1, 447–462. doi:10.1007/s44164-022-00038-5.

BIU Publications 2021

Lawson MJ, Katsamenis OL, Chatelet D, Alzetani A, Larkin O, Haig I, Lackie P, Warner J, Schneider P. Immunofluorescence-guided segmentation of three-dimensional features in micro-computed tomography datasets of human lung tissue. R Soc Open Sci. 2021 Nov 3;8(11):211067. doi: 10.1098/rsos.211067. PMID: 34737879; PMCID: PMC8564621.

Caetano L, Eckert JJ, Johnston D, Chatelet DS, Tumbarello DA, Smyth NR, Ingamells S, Price A, Fleming TP. Blastocyst trophectoderm endocytic activation, a marker of adverse developmental programming. Reproduction. 2021 Sep 6;162(4):289-306. doi: 10.1530/REP-21-0234. PMID: 34338217.

Gahleitner F, Thompson J, Jackson CL, Hueppe JF, Behan L, Dehlink E, Goutaki M, Halbeisen F, Queiroz APL, Thouvenin G, Kuehni CE, Latzin P, Lucas JS, Rubbo B. Lower airway clinical outcome measures for use in primary ciliary dyskinesia research: a scoping review. ERJ Open Res. 2021 Nov 29;7(4):00320-2021. doi: 10.1183/23120541.00320-2021. PMID: 34853782.

Pedersen ESL, Collaud ENR, Mozun R, Dexter K, Kruljac C, Silberschmidt H, Lucas JS (2021) COVID-PCD patient advisory group; Goutaki M, Kuehni CE. Facemask Usage Among People With Primary Ciliary Dyskinesia During the COVID-19 Pandemic: A Participatory Project. Int J Public Health Dec 15;66:1604277. doi: 10.3389/ijph.2021.1604277. PMID: 34975364.

Wheway G, Thomas NS, Carroll M, Coles J, Doherty R; Genomics England Research Consortium; Goggin P, Green B, Harris A, Hunt D, Jackson CL, Lord J, Mennella V, Thompson J, Walker WT, Lucas JS. (2021) Whole genome sequencing in the diagnosis of primary ciliary dyskinesia. BMC Med Genomics 14(1):234. doi: 10.1186/s12920-021-01084-w. PMID: 34556108; PMCID.

Franco-Bocanegra DK, Gourari Y, McAuley C, Chatelet DS, Johnston DA, Nicoll JAR, Boche D (2021). Microglial morphology in Alzheimer’s disease and after Aβ immunotherapy. Scientific Reports, 11(1); 1-12. doi: 10.1038/s41598-021-95535-0.

Pedersen ESL, Mallet MC, Lam YT, Bellu S, Cizeau I, Copeland F, Fernandez TL, Manion M, Harris AL, Lucas JS, Santamaria F, Goutaki M, Kuehni CE, COVID-PCD Patient Advisory Group (2021). COVID-19 Vaccinations: Perceptions and Behaviours in People with Primary Ciliary Dyskinesia. Vaccines (Basel). 9(12):1496 Dec 17;9(12):1496. doi: 10.3390/vaccines9121496.

De Picker LJ, Victoriano GM, Richards R, Gorvett AJ, Lyons S, Buckland GR, Tofani T, Norman JL, Chatelet DS, Nicoll JAR, Boche D (2021) Immune environment of the brain in schizophrenia and during the psychotic episode: A human post-mortem study. Brain Behav Immun 97:319-327. doi: 10.1016/j.bbi.2021.07.017. Epub 2021 Jul 30. PMID: 34339805.

Lynn SA, Johnston DA, Scott JA, Munday R, Desai RS, Keeling E, Weaterton R, Simpson A, Davis D, Freeman T, Chatelet DS, Page A, Cree AJ, Lee H, Newman TA, Lotery AJ, Ratnayaka JA (2021) Oligomeric Aβ1-42 Induces an AMD-Like Phenotype and Accumulates in Lysosomes to Impair RPE Function. Cells 17;10(2):413. doi: 10.3390/cells10020413. PMID: 33671133.

Harris SE, Matthews KS, Palaiologou E, Tashev SA, Lofthouse EM, Pearson-Farr J, Goggin P, Chatelet DS, Johnston DA, Jongen MS, Page AM, Cleal JK, Lewis RM (2021) Pericytes on placental capillaries in terminal villi preferentially cover endothelial junctions in regions furthest away from the trophoblast. Placenta. 104:1-7. doi: 10.1016/j.placenta.2020.10.032. PMID: 33190063.

Blume C, Jackson CL, Spalluto CM, Legebeke J, Nazlamova L, Conforti F, Perotin JM, Frank M, Butler J, Crispin M, Coles J, Thompson J, Ridley RA, Dean LSN, Loxham M, Reikine S, Azim A, Tariq K, Johnston DA, Skipp PJ, Djukanovic R, Baralle D, McCormick CJ, Davies DE, Lucas JS, Wheway G, Mennella V. (2021) A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection. Nat Genet. 53(2):205-214. doi: 10.1038/s41588-020-00759-x. Epub 2021 Jan 11. PMID: 33432184.

Gui M, Farley H, Anujan P, Anderson JR, Maxwell DW, Whitchurch JB, Botsch JJ, Qiu T, Meleppattu S, Singh SK, Zhang Q, Thompson J, Lucas JS, Bingle CD, Norris DP, Roy S, Brown A (2021) De novo identification of mammalian ciliary motility proteins using cryo-EM. Cell 184(23):5791-5806.e19. doi: 10.1016/j.cell.2021.10.007. Epub 2021 Oct 28. PMID: 34715025.

Georgiadou N, Singh S, Wagner B, Goggin P, Katsarma E, Singh M (2021) Occupational Localized Cutaneous Argyria With Pseudo-Ochronosis in a Jeweler. Am J Dermatopathol 43(11):822-826. doi: 10.1097/DAD.0000000000001921. PMID: 33606379.