Publications from the BIU

Being a core service facility, BIU staff rarely have time to undertake their own research projects.

However, we do make a significant contributions to many ongoing research projects which results in co-authorship of numerous scientific papers each year - recent publications are shown below with BIU staff shown in bold. We also provide, lesser advice, support and imaging services to many other projects, resulting in the BIU being acknowledged in the publications from those projects.

We endorse both the Royal Microscopical Society's Core Facilities Publication Policy concerning the crediting of imaging facility staff for their contribution to published works and the University of Southampton's Fair Attribution Guidance

If you do publish work that includes data from our instruments, please do acknowledge the BIU in your paper and send us a copy for our records.

BIU Publications 2021

• De novo identification of mammalian ciliary motility proteins using cryo-EM. Gui M, Farley H, Anujan, P, Anderson JR, Maxwell DW, Whitchurch JB, Botsch JJ, Qui T, Meleppattu S, Singh SK, Zhang Q, Thompson J, Lucas JS, Bingle CD, Norris D, Roy S and Brown A. Cell . 2021 Oct 21;S0092-8674(21)01181-8. doi: 10.1016/j.cell.2021.10.007. Online ahead of print.
• Quantitative assessment of dentine mineralization and tubule occlusion by NovaMin and stannous fluoride using serial block face scanning electron microscopy. Mahmoodi B, Goggin P, Fowler C, Cook RB. J Biomed Mater Res B Appl Biomater. 2021 May;109(5):717-722. doi: 10.1002/jbm.b.34737. Epub 2020 Oct 16. PMID: 33063467.
• Immune environment of the brain in schizophrenia and during the psychotic episode: A human post-mortem study. De Picker LJ, Victoriano GM, Richards R, Gorvett AJ, Lyons S, Buckland GR, Tofani T, Norman JL, Chatelet DS, Nicoll JAR, Boche D. Brain Behav Immun. 2021 Oct;97:319-327. doi: 10.1016/j.bbi.2021.07.017. Epub 2021 Jul 30. PMID: 34339805.
• Occupational Localized Cutaneous Argyria With Pseudo-Ochronosis in a Jeweler. Georgiadou N, Singh S, Wagner B, Goggin P, Katsarma E, Singh M. Am J Dermatopathol. 2021 Nov 1;43(11):822-826. doi: 10.1097/DAD.0000000000001921. PMID: 33606379.
• Whole genome sequencing in the diagnosis of primary ciliary dyskinesia. Wheway G, Thomas NS, Carroll M, Coles J, Doherty R; Genomics England Research Consortium, Goggin P, Green B, Harris A, Hunt D, Jackson CL, Lord J, Mennella V, Thompson J, Walker WT, Lucas JS. BMC Med Genomics. 2021 Sep 23;14(1):234. doi: 10.1186/s12920-021-01084-w. PMID: 34556108.
• Blastocyst trophectoderm endocytic activation, a marker of adverse developmental programming. Caetano L, Eckert JJ, Johnston D, Chatelet DS, Tumbarello DA, Smyth NR, Ingamells S, Price A, Fleming TP. Reproduction. 2021 Sep 6;162(4):289-306. doi: 10.1530/REP-21-0234. PMID: 34338217.
• Microglial morphology in Alzheimer's disease and after Aβ immunotherapy. Franco-Bocanegra DK, Gourari Y, McAuley C, Chatelet DS, Johnston DA, Nicoll JAR, Boche D. Sci Rep. 2021 Aug 5;11(1):15955. doi: 10.1038/s41598-021-95535-0. PMID: 34354209.
• Pericytes on placental capillaries in terminal villi preferentially cover endothelial junctions in regions furthest away from the trophoblast.  Harris SE, Matthews KS, Palaiologou E, Tashev SA, Lofthouse EM, Pearson-Farr J, Goggin P, Chatelet DS, Johnston DA, Jongen MS, Page AM, Cleal JK, Lewis RM. Placenta. 2021 Jan 15;104:1-7. doi: 10.1016/j.placenta.2020.10.032. Epub 2020 Nov 7. PMID: 33190063
• A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection. Blume C, Jackson CL, Spalluto CM, Legebeke J, Nazlamova L, Conforti F, Perotin J-M, Frank M, Butler J, Crispin M, Coles J, Thompson J, Ridley RA, Dean LSN,Loxham M, Reikine S, Azim A, Tariq K, Johnston DA, Skipp PJ, Djukanovic R, Baralle D, McCormick CJ, Davies DE, Lucas JS, Wheway G, Mennella V. Nature Genetics . 2021 Feb;53(2):205-214. doi: 10.1038/s41588-020-00759-x. Epub 2021 Jan 11.
• Oligomeric Aβ1-42 Induces an AMD-Like Phenotype and Accumulates in Lysosomes to Impair RPE Function. Lynn SA, Johnston DA, Scott JA, Munday R, Desai RS, Keeling E, Weaterton R, Simpson A, Davis D, Freeman T, Chatelet DS, Page A, Cree AJ, Lee H, Newman TA, Lotery AJ, Ratnayaka JA. Cells. 2021 Feb 17;10(2):413. doi: 10.3390/cells10020413. PMID: 33671133.
• TNF receptor agonists induce distinct receptor clusters to mediate differential agonistic activity. Yu X, James S, Felce JH, Kellermayer B, Johnston DA, Chan HTC, Penfold CA, Kim J, Inzhelevskaya T, Mockridge CI, Watanabe Y, Crispin M, French RR, Duriez PJ, Douglas LR, Glennie MJ, Cragg MS. Communications Biology 2021 Jun 23;4(1):772. doi: 10.1038/s42003-021-02309-5. PMID: 34162985.

BIU Publications 2020

• 3D-reconstructed retinal pigment epithelial cells provide insights into aging and pathology in the outer retina Keeling E, Chatelet DS, Tan NYT, Khan F, Richards R, Thisainathan T, Goggin P, Page A, Tumbarello DA, Lotery AJ, Ratnayaka JA. International Journal of Molecular Science . 2020 Nov 9;21(21):8408.
•  A Revised Protocol for Culture of Airway Epithelial Cells as a Diagnostic Tool for Primary Ciliary Dyskinesia Coles, JL, Thompson J,  Horton KL, Hirst RA, Griffin P, Williams GW, Jackson CL, Blume C, Goggin P, Doherty R, Lackie PM, Harris A, Walker WT, O’Callaghan C, Hogg C, Lucas JS. Journal of Clinical Medicine 2020 Nov 21:9(11):3753.
•  An In-Vitro Cell Model of Intracellular Protein Aggregation Provides Insights into RPE Stress Associated with Retinopathy. Keeling E, Culling AJ, Johnston DA, Chatelet DS, Page A, Tumbarello DA, Lotery AJ, Ratnayaka JA. International Journal of Molecular Science 2020 Sep 11;21(18):6647. doi: 10.3390/ijms21186647. PMID: 32932802.
• Antimicrobial Activity of the Quinoline Derivative HT61 against Staphylococcus aureus Biofilms. Frapwell CJ, Skipp PJ, Howlin RP, Angus EM, Hu Y, Coates ARM, Allan RN, Webb JS. Antimicrobial Agents and Chemotherepy. 2020 Apr 21;64(5):e02073-19. doi: 10.1128/AAC.02073-19. Print 2020 Apr 21. PMID: 32122902.
• Anti-PD-1 immunotherapy leads to tuberculosis reactivation via dysregulation of TNF. Tezera LB, Bielecka MK, Ogongo P, Walker NF, Ellis M, Garay-Baquero DJ, Thomas K, Reichmann MT, Johnston DA, Wilkinson KA, Ahmed M, Jogai S, Jayasinghe SN, Wilkinson RJ, Mansour S, Thomas GJ, Ottensmeier CH, Leslie A, Elkington PT (2020) -α. Elife. 2020 Feb 24;9:e52668. doi: 10.7554/eLife.52668.
• ApoE4 Astrocytes Secrete Basement Membranes Rich in Fibronectin and Poor in Laminin Compared to ApoE3 Astrocytes. Keable A, O'Neill R, MacGregor Sharp M, Gatherer M, Yuen HM, Johnston DA, Weller RO, Carare RO. International Journal of Molecular Science. 2020 Jun 19;21(12):4371. doi: 10.3390/ijms21124371. PMID: 32575521.
• Clinical utility of NGS diagnosis and disease stratification in a multiethnic primary ciliary dyskinesia cohort. Fassad MR, Patel MP, Shoemark A, Cullup T, Hayward J, Dixon M, Rogers AV, Ollosson S, Jackson C, Goggin P, Hirst RA, Fasseeh N, Lucas JS, Hogg C, Mitchison HM. J Med Genet. 2020 May;57(5):322-330.
• Development of protocols for the first serial block-face scanning electron microscopy (SBF SEM) studies of bone tissue. Goggin P, Ho EML, Gnaegi H, Searle S, Oreffo ROC, Schneider P. Bone 2020 Feb;131:115107. doi: 10.1016/j.bone.2019.115107. Epub 2019 Oct 24. PMID: 31669251.
• Evolutionary dynamics of genome size in a radiation of woody plants. Moeglein MK, Chatelet DS, Donoghue MJ, Edwards EJ. Am J Bot. 2020 Nov;107(11):1527-1541. doi: 10.1002/ajb2.1544. Epub 2020 Oct 20. PMID: 33079383.
• Human placental villi contain stromal macrovesicles associated with networks of stellate cells. Palaiologou E, Etter O, Goggin P, Chatelet DS, Johnston DA, Lofthouse EM, Doherty R, Pearson-Farr J, Sengers BG, Torrens C, Cleal JK, Page AM, Lewis RM (2020). Journal of  anatomy doi: 10.1111/joa.13082. [Epub ahead of print].
• Occupational localised cutaneous argyria with pseudo-ochronosis in a jeweller.Georgiadou, N, Singh, S, Wagner, B,  Goggin P, Katsarma, Singh EM (2020) American Journal of Dermatopathology 1 Feb 16. doi: 10.1097/DAD.0000000000001921.
• Peri-arterial pathways for clearance of α-Synuclein and tau from the brain: Implications for the pathogenesis of dementias and for immunotherapy. Nimmo J, Johnston DA, Dodart JC, MacGregor-Sharp MT, Weller RO, Nicoll JAR, Verma A, Carare RO.. Alzheimers Dement (Amst). 2020 Jul 28;12(1):e12070. doi: 10.1002/dad2.12070. eCollection 2020. PMID: 32782922.
• Pericytes on placental capillaries in terminal villi preferentially cover endothelial in regions furthest away from the trophoblast Harris, SE, Matthews, J, Palaiologou E, Tashev SA, Lofthouse EM, Pearson-Farr J, Goggin P, Jongen MS, Page AM, Cleal JK, Lewis RM Placenta. 2020 Nov 7;104:1-7.
• Quantitative Assessment of Dentine Mineralisation and Tubule Occlusion by Novamin® and Stannous Fluoride Using Serial Block Face Scanning Electron Microscopy. Mahmoodi B, Goggin P, Fowler C and Cook R. Journal of Biomedical Materials Research B Applied Biomaterials. 2020 Oct 16. doi: 10.1002/jbm.b.34737.
• Serial block-face scanning electron microscopy reveals novel intercellular connections in human term placental microvasculature. Palaiologou E, Goggin P, Chatelet DS, Ribeiro de Souza R, Chiu W, Ashley B, Lofthouse EM, Sengers BG, Torrens C, Page AM, Cleal JK, Lewis RM. Journal of Anatomy 2020 Aug;237(2):241-249. doi: 10.1111/joa.13191. Epub 2020 Apr 3. PMID: 32242928.
• The Role of Cholesterol on Triterpenoid Saponin-Induced Endolysosomal Escape of a Saporin-Based Immunotoxin. Smith WS, Johnston DA, Wensley HJ, Holmes SE, Flavell SU, Flavell DJ. International Journal of Molecular Science 2020 Nov 19;21(22):8734. doi: 10.3390/ijms21228734. PMID: 33228031.

BIU Publications 2019

• A Flow Cytometric Method to Quantify the Endosomal Escape of a Protein Toxin to the Cytosol of Target Cells. Wensley HJ, Johnston DA, Smith WS, Holmes SE, Flavell SU, Flavell DJ Pharmacological Research 2019 Dec 23;37(1):16. doi: 10.1007/s11095-019-2725-1.
• A lasered mouse model of retinal degeneration displays progressive outer retinal pathology providing insights into early geographic atrophy. Ibbett P, Goverdhan SV, Pipi E, Chouhan JK, Keeling E, Angus EM, Scott JA, Gatherer M, Page A, Teeling JL, Lotery AJ, Arjuna Ratnayaka J. Scientific Reports. 2019 May 16;9(1):7475. doi: 10.1038/s41598-019-43906-z. PMID: 31097765.
• A novel application of Gini coefficient for the quantitative measurement of bacterial aggregation. Cai YM, Chatelet DS, Howlin RP, Wang ZZ, Webb JS. Scientific Reports 2019 Dec 12;9(1):19002. doi: 10.1038/s41598-019-55567-z. PMID: 31831832.
• Augmentation of Saporin-Based Immunotoxins for Human Leukaemia and Lymphoma Cells by Triterpenoid Saponins: The Modifying Effects of Small Molecule Pharmacological Agents. Smith WS, Johnston DA, Holmes SE, Wensley HJ, Flavell SU, Flavell DJ. Toxins (Basel). 2019 Feb 20;11(2):127. doi: 10.3390/toxins11020127. PMID: 30791598.
• Oxidative Stress and Dysfunctional Intracellular Traffic Linked to an Unhealthy Diet Results in Impaired Cargo Transport in the Retinal Pigment Epithelium (RPE). Keeling E, Chatelet DS, Johnston DA, Page A, Tumbarello DA, Lotery AJ, Ratnayaka JA. Molecular Nutrition and Food Research. 2019 Aug;63(15):e1800951. doi: 10.1002/mnfr.201800951. Epub 2019 Mar 20. PMID: 30835933.
• Risk factors for situs defects and congenital heart disease in primary ciliary dyskinesia. Best S, Shoemark A, Rubbo B, Patel MP, Fassad MR, Dixon M, Rogers AV, Hirst RA, Rutman A, Ollosson S, Jackson CL, Goggin P, Thomas S, Pengelly R, Cullup T, Pissaridou E, Hayward J, Onoufriadis A, O'Callaghan C, Loebinger MR, Wilson R, Chung EM, Kenia P, Doughty VL, Carvalho JS, Lucas JS, Mitchison HM, Hogg C. Thorax. 2019 Feb;74(2):203-205. doi: 10.1136/thoraxjnl-2018-212104. Epub 2018 Aug 30. PMID: 30166424.
• Self-tattooing of eyeball with inadvertent corneoscleral perforation: the implication of social media. Ng JY, Ting DSJ, Vaideanu-Collins D, Mudhar HS, Wagner B, Goggin P, Gibson A, Patel T. Eye (London). 2019 Oct;33(10):1672-1674. doi: 10.1038/s41433-019-0472-5. Epub 2019 Jun 5. PMID: 31168058.
• Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells. Liu Z, Nguyen QPH, Nanjundappa R, Delgehyr N, Megherbi A, Doherty R, Thompson J, Jackson C, Albulescu A, Heng YM, Lucas JS, Dell SD, Meunier A, Czymmek K, Mahjoub MR, Mennella V. Developmental Cell 2020 Oct 26;55(2):224-236.e6. doi: 10.1016/j.devcel.2020.09.016. Epub 2020 Oct 9. PMID: 33038333.
• X-ray Micro-Computed Tomography for Nondestructive Three-Dimensional (3D) X-ray Histology. Katsamenis OL, Olding M, Warner JA, Chatelet DS, Jones MG, Sgalla G, Smit B, Larkin OJ, Haig I, Richeldi L, Sinclair I, Lackie PM, Schneider P. American Journal of Pathology. 2019 Aug;189(8):1608-1620. doi: 10.1016/j.ajpath.2019.05.004. Epub 2019 May 22. PMID: 31125553.

BIU Publications 2018

•  A convenient protocol for establishing a human cell culture model of the outer retina. Lynn SA, Keeling E, Dewing JM, Johnston DA, Page A, Cree AJ, Tumbarello DA, Newman TA, Lotery AJ, Ratnayaka JA. F1000Research 2018 Jul 18;7:1107. doi: 10.12688/f1000research.15409.1. eCollection 2018. PMID: 30271583.
•  A novel method to visualise the three-dimensional organisation of the human cerebral cortical vasculature. Harrison CH, Buckland GR, Brooks SE, Johnston DA, Chatelet DS, Liu AKL, Gentleman SM, Boche D, Nicoll JAR. Journal of Anatomy 2018 Jun;232(6):1025-1030. doi: 10.1111/joa.12805. Epub 2018 Mar 9. PMID: 29520782.
•  Collective Cell Behavior in Mechanosensing of Substrate Thickness. Tusan CG, Man YH, Zarkoob H, Johnston DA, Andriotis OG, Thurner PJ, Yang S, Sander EA, Gentleman E, Sengers BG, Evans ND. Biophysics Journal 2018 Jun 5;114(11):2743-2755. doi: 10.1016/j.bpj.2018.03.037. PMID: 29874622.
•  Convective influx/glymphatic system: tracers injected into the CSF enter and leave the brain along separate periarterial basement membrane pathways. Albargothy NJ, Johnston DA, MacGregor-Sharp M, Weller RO, Verma A, Hawkes CA, Carare RO. Acta Neuropathology 2018 Jul;136(1):139-152. doi: 10.1007/s00401-018-1862-7. Epub 2018 May 12. PMID: 29754206.
•  High prevalence of CCDC103 p.His154Pro mutation causing primary ciliary dyskinesia disrupts protein oligomerisation and is associated with normal diagnostic investigations. Shoemark A, Moya E, Hirst RA, Patel MP, Robson EA, Hayward J, Scully J, Fassad MR, Lamb W, Schmidts M, Dixon M, Patel-King RS, Rogers AV, Rutman A, Jackson CL, Goggin P, Rubbo B, Ollosson S, Carr S, Walker W, Adler B, Loebinger MR, Wilson R, Bush A, Williams H, Boustred C, Jenkins L, Sheridan E, Chung EMK, Watson CM, Cullup T, Lucas JS, Kenia P, O'Callaghan C, King SM, Hogg C, Mitchison HM. Thorax. 2018 Feb;73(2):157-166. doi: 10.1136/thoraxjnl-2017-209999. Epub 2017 Aug 8. PMID: 28790179.
•  Nanoscale dysregulation of collagen structure-function disrupts mechano-homeostasis and mediates pulmonary fibrosis. Jones MG, Andriotis OG, Roberts JJW, Lunn K, Tear VJ, Cao L, Ask, K, Smart DE, Bonfanti A, Johnson P,  lzetani A, Conforti F, Doherty R, Lai CY, Johnson B, Bourdakos KN, Fletcher SV, Marshall BG, Jogai S, Brereton CJ, Chee, SJ, Ottensmeier CH, Sime, P, Gauldie J, Kolb M, Mahajan S, Fabre A, Bhaskar A, Jarolimek W, Richeldi L, O'Reilly KM, Monk PD, Thurner PJ, Davies DE. Elife 2018 Jul 3;7:e36354. doi: 10.7554/eLife.36354.
•  Study of Intracellular Cargo Trafficking and Co-localization in the Phagosome and Autophagy-Lysosomal Pathways of Retinal Pigment Epithelium (RPE) Cells. Ratnayaka JA, Keeling E, Chatelet DS. Methods in Molecular Biology 2020;2150:167-182. doi: 10.1007/7651_2019_223. PMID: 30969403.