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The University of Southampton
Biomedical Imaging UnitEquipment and Facilities

Confocal and Lightsheet MIcroscopy

Confocal microscopy uses a pinhole in the light path to discard signal from out of focus regions of a fluorescently labelled (or reflective) sample, thereby generating a sharply focussed, optical section through the sample. It is a high magnification, high resolution imaging method which uses a scanning laser beam to excite the fluorescence. Refocussing the microscope and reimaging allows 3D datasets to be collected. Our Leica confocal systems have true spectral detection allowing the simultaneous recording of 4, non-overlapping colour ranges with infinite flexibility and nanometre precision in terms of wavelength detection range.

View a gallery of our confocal microscopy images here.

Lightsheet microscopy, otherwise known as selective plane illumination microscopy (SPIM), is a lower magnification, lower resolution method for producing optical sections and 3D images from larger samples (up to 10 x 10 x 5mm). It uses a wide, thin sheet of laser light entering the sample from the side (or from multiple sides) to excite fluorescence at a single focal plane which is imaged using a high resolution, ultra sensitive scientific camera. Moving the sample through the laser beam allows signal from different levels within the sample to be captured and 3D data sets generated. Samples for light sheet microscopy need considerable processing to render them optically clear.

Find out more about lightsheet microscopy here.

 

Leica TCS-SP8 Confocal Microscope
Leica TCS-SP8 Confocal Microscope

 Leica TCS-SP8 Laser Scanning Confocal Microscope

Leica TCS-SP5 Confocal Microscope
Leica TCS-SP5 Confocal Microscope

 Leica SP5 Laser Scanning Confocal Microscope

LaVision Ultramicroscope Light Sheet System
LaVision Ultramicroscope Light Sheet System

 LaVision Light Sheet Ultramicroscope

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