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The University of Southampton
Biomedical Imaging UnitEquipment and Facilities

Scanning Electron Microscopy

Scanning electron microscopy uses a beam of electrons to scan across the surface of a sample and image the surface topography at high resolution and with a large depth of focus. Conventionally, the electron beam interacts with the surface of the specimen (which has been coated with an ultrathin layer of metal) to release secondary electrons which are detected to create the image although backscattered (reflected) electrons from the scanning beam can also be detected. The electron beam also generates x-rays from the sample which can be detected and characterised to identify and map the elemental composition of the sample. Most imaging is done using specially dried and coated samples under high vacuum but low vacuum and environmental modes can be used on some uncoated samples.

View a gallery of our scanning electron microscopy images here.

Our ultra high resolution FEI Quanta 250 SEM is equipped with a Gatan 3View II serial block face imaging system for 3D imaging of samples. Find out more about serial block face imaging here.

FEI Quanta 250 SEM
FEI Quanta 250 SEM

 FEI Quanta 250 Scanning Electron Microscope with Gatan 3view2XP serial block face imaging

FEI Quanta 200 SEM
FEI Quanta 200 SEM

 FEI Quanta 200 Scanning Electron Microscope

 

Quorum Q150T ES coater
Quorum Q150T ES coater

Scanning Electron Microscopy Sample Processing

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