The University of Southampton
Courses

BIOL6075 Biological Optical Imaging

Module Overview

Aims and Objectives

Module Aims

Outcome Students will gain an understanding of the components and optical pathways used in core biological imaging systems which will enable them to efficiently operate advanced microscopes. Understand the theoretical concepts, capabilities and limitations of core biological imaging modalities and their associated techniques and applications. Outcome Students will gain the ability to select and configure the correct imaging system for specific experimental applications with an expectation of the type and quality of resulting data. Theoretical knowledge and practical proficiency in the fundamental chemistry and handling required to prepare specimens for transmitted light, fluorescence and live imaging applications. Outcome Essential handling and biochemistry skills required for the preparation of samples for optical biological imaging that can be developed and adapted for specific experimental applications. Identify and interpret canonical cell biology markers as viewed through a microscope. Outcome Students will develop the ability to select appropriate marker stains for imaging experiments and identify structures and organelles from their microscopic appearance. Hands-on experience with a range of core biological imaging modalities. Outcome Students will gain the basic skills required to operate core imaging modalities and also handle and calibrate raw datasets for analysis. Derive quantitative data from biological images. Outcome Students will learn the analytical options available to them to derive quantitative data for statistical analysis of biological images. They will be able to make basic measurements such as area, length and density as well as use colocalisation and image segmentation algorithms.

Learning Outcomes

Learning Outcomes

Having successfully completed this module you will be able to:

  • Understand the hardware specifications of biological imaging equipment Outcome Students will gain an understanding of the components and optical pathways used in core biological imaging systems which will enable them to efficiently operate advanced microscopes.
  • Understand the theoretical concepts, capabilities and limitations of core biological imaging modalities and their associated techniques and applications. Outcome Students will gain the ability to select and configure the correct imaging system for specific experimental applications with an expectation of the type and quality of resulting data.
  • Theoretical knowledge and practical proficiency in the fundamental chemistry and handling required to prepare specimens for transmitted light, fluorescence and live imaging applications. Outcome Essential handling and biochemistry skills required for the preparation of samples for optical biological imaging that can be developed and adapted for specific experimental applications.
  • Identify and interpret canonical cell biology markers as viewed through a microscope. Outcome Students will develop the ability to select appropriate marker stains for imaging experiments and identify structures and organelles from their microscopic appearance.
  • Hands-on experience with a range of core biological imaging modalities. Outcome Students will gain the basic skills required to operate core imaging modalities and also handle and calibrate raw datasets for analysis.
  • Derive quantitative data from biological images. Outcome Students will learn the analytical options available to them to derive quantitative data for statistical analysis of biological images. They will be able to make basic measurements such as area, length and density as well as use colocalisation and image segmentation algorithms.

Syllabus

The course consists of both practical and theoretical components although the principal focus will be on practical components. The theoretical component will consist of a series of lectures and e-learning resources, which will precede their associated practicals. The practical component will comprise of hands-on laboratory practicals in which students will work in groups and workshops in which students will work independently.

Learning and Teaching

TypeHours
Lecture5
Independent Study75
Practical classes and workshops35
Total study time115

Assessment

Formative

Oral presentation

Summative

MethodPercentage contribution
Oral presentation 15%
Practical 85%
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