Publications

Recent publications from the Environmental Healthcare Unit.

• Warnes, Sarah L. , Summersgill, Emma N. and Keevil, C William (2015) Inactivation of murine norovirus on a range of copper alloy surfaces is accompanied by loss of capsid integrity. Applied and Environmental Microbiology , 81 , (3) , 1085-1091 . ( doi:10.1128/AEM.03280-14 ). (PMID:25452290).
• Phippen, Curtis William , Mikolajek, Halina , Schlaefli, Henry George , Keevil, Charles William , Webb, Jeremy Stephen and Tews, Ivo (2014) Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator. FEBS Letters ( doi:10.1016/j.febslet.2014.11.002 ). (In Press).
• Meier, Alexandra , Tsaloglou, Nefeli-Maria , Mowlem, Matthew C. , Keevil, C. William and Connelly, Douglas P. (2013) Hyperbaric biofilms on engineering surfaces formed in the deep sea. Biofouling , 29 , (9) , 1029-1042 . ( doi:10.1080/08927014.2013.824967 ).
• Warnes, Sarah L. and Keevil, C. William (2013) Inactivation of norovirus on dry copper alloy surfaces. PLoS ONE , 8 , (9) , 1-9 . ( doi:10.1371/journal.pone.0075017 ). (PMID:24040380).
Cerqueira, Laura , Fernandes, Ricardo M. , Ferreira, Rui M. , Oleastro, Mónica , Carneiro, Fátima , Brandao, Catarina , Pimentel-Nunes, Pedro , Dinis-Ribeiro, Mário , Figueiredo, Céu , Keevil, Charles W. , Vieira, Maria J. and Azevedo, Nuno F. (2013) Validation of a PNA-FISH method for the detection of Helicobacter pylori clarithromycin resistance in gastric biopsies. Journal of Clinical Microbiology ( doi:10.1128/JCM.00302-13 ). (PMID:23596234).
• Almeida, C. , Azevedo, N. F. , Bento, J. C. , Cerca, N. , Ramos, H. , Vieira, M. J. and Keevil, C. W. (2013) Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH. European Journal of Clinical Microbiology & Infectious Diseases ( doi:10.1007/s10096-012-1808-2 ).
• Warnes, S. L. , Highmore, C. J. and Keevil, C. W. (2012) Horizontal transfer of antibiotic resistance genes on abiotic touch surfaces: implications for public health. mBio , 3 , (6) , e00489-[12 pages] . ( doi:10.1128/mBio.00489-12 ).
• Gião, M.S. and Keevil, C.W. (2012) Hydrodynamic shear stress to remove Listeria monocytogenesbiofilms from stainless steel and polytetrafluoroethylene surfaces. Journal of Applied Microbiology , 114 , (1) , 256-265 . ( doi:10.1111/jam.12032 ).
• Azevedo, Nuno F. , Bragança, Sofia M. , Simões, Lúcia C. , Cerqueira, Laura , Almeida, Carina , Keevil, Charles W. and Vieira, Maria J. (2012) Proposal for a method to estimate nutrient shock effects in bacteria. BMC Research Notes , 5 , (1) , 422 . ( doi:10.1186/1756-0500-5-422 ).
• Warnes, S.L. , Caves, V. and Keevil, C.W. (2012) Mechanism of copper surface toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarization followed by slower rate of DNA destruction which differs from that observed for Gram-positive bacteria. Environmental Microbiology , 14 , (7) , 1730-1743 . ( doi:10.1111/j.1462-2920.2011.02677.x ). (PMID:22176893).
• Warnes, S.L. , Caves, V. and Keevil, C.W. (2012) Mechanism of copper surface toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarization followed by slower rate of DNA destruction which differs from that observed for Gram-positive bacteria. Environmental Microbiology , 14 , (7) , 1730-1743 . ( doi:10.1111/j.1462-2920.2011.02677.x ). (PMID:22176893).
• Secker, T.J. , Hervé, R. , Zhao, Q. , Borisenko, K.B. , Abel, E.W. and Keevil, C.W. (2012) Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning. Biofouling , 28 , (6) , 563-569 . ( doi:10.1080/08927014.2012.698387 ). (PMID:22694725).
• Secker, T.J. , Hervé, R. and Keevil, C.W. (2012) Letter to the editor. Response to the letter from M. Feckie. Journal of Hospital Infection , 80 , (2) , 181-182 . ( doi:10.1016/j.jhin.2011.11.006 ).
• Hervé, R. and Keevil, C.W. (2012) Current limitations about the cleaning of luminal endoscopes. Journal of Hospital Infection , 83 , (1) , 22-29 . ( doi:10.1016/j.jhin.2012.08.008 ). (PMID:23098682).
• Collins, Samuel L. , Hervé, Rodolphe , Keevil, C.W. , Blaydes, Jeremy P. and Webb, Jeremy S. (2011) Down-regulation of DNA mismatch repair enhances initiation and growth of neuroblastoma and brain tumour multicellular spheroids. PLoS ONE , 6 , (12) , e28123 . ( doi:10.1371/journal.pone.0028123 ). (PMID:22145025).
• Azevedo, N.F. , Jardim, T. , Almeida, C. , Cerqueira, L. , Almeida, A.J. , Rodrigues, F. , Keevil, C W. and Vieira, M.J. (2011) Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples. Antonie van Leeuwenhoek , 100 , (3) , 463-470 . ( doi:10.1007/s10482-011-9595-9 ). (PMID:21638111).
• Warnes, S.L. and Keevil, C.W. (2011) Mechanism of copper surface toxicity in vancomycin-resistant enterococci following wet or dry surface contact. Applied and Environmental Microbiology , 77 , (17) , 6049-6059 . ( doi:10.1128/AEM.00597-11 ). (PMID:21742916).
• Secker, T.J. , Hervé, R. and Keevil, C.W. (2011) Adsorption of prion and tissue proteins to surgical stainless steel surfaces and the efficacy of decontamination following dry and wet storage conditions. Journal of Hospital Infection , 78 , (4) , 251-255 . ( doi:10.1016/j.jhin.2011.03.021 ). (PMID:21658801).
• Cerqueira, Laura , Fernandes, Ricardo M.. , Ferreira, Rui M. , Carneiro, Fátima , Dinis-Ribeiro, Mário , Figueiredo, Céu , Keevil, Charles W. , Azevedo, Nuno F. and Vieira, Maria J. (2011) PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori. BMC Microbiology , 11 , (1) , 101-[7pp] . ( doi:10.1186/1471-2180-11-101 ).
• Almeida, Carina , Azevedo, Nuno F. , Santos, Sílvio , Keevil, Charles W. and Vieira, Maria J. (2011) Discriminating multi-species populations in biofilms with peptide nucleic acid fluorescence in situ hybridization (PNA FISH). PLoS ONE , 6 , (3) , e14786-[13pp] . ( doi:10.1371/journal.pone.0014786 ).
• Gião, Maria S. , Azevedo, Nuno F. , Wilks, Sandra A. , Vieira, Maria J. and Keevil, Charles W. (2011) Interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms. BMC Microbiology , 11 , (1) , 57-[10pp] . ( doi:10.1186/1471-2180-11-57 ).
• Weaver, L. , Noyce, J.O. , Michels, H.T and Keevil, C.W. (2010) Potential action of copper surfaces on meticillin-resistant Staphylococcus aureus. Journal of Applied Microbiology , 109 , (6) , 2200-2205 . ( doi:10.1111/j.1365-2672.2010.04852.x ). (PMID:21040269).
• Hervé, R. , Secker, T.J. and Keevil, C.W. (2010) Current risk of iatrogenic Creutzfeld–Jakob disease in the UK: efficacy of available cleaning chemistries and reusability of neurosurgical instruments. Journal of Hospital Infection , 75 , (4) , 309-313 . ( doi:10.1016/j.jhin.2010.01.024 ). (PMID:20451298).
• Warnes, S.L. , Gree, S.M. , Michels, H.T. and Keevil, C.W. (2010) Biocidal efficacy of copper alloys against pathogenic enterococci involves degradation of genomic and plasmid DNAs. Applied and Environmental Microbiology , 76 , (16) , 5390-5401 . ( doi:10.1128/AEM.03050-09 ). (PMID:20581191).
• Almeida, C. , Azevedo, Nuno F. , Fernandes, R.M. , Keevil, Charles W. and Vieira, M.J. (2010) A fluorescence in situ hybridization method using a peptide nucleic acid probe for the identification of Salmonella spp. in a broad spectrum of samples. Applied and Environmental Microbiology ( doi:10.1128/AEM.01678-09 ).
• Howlin, R.P. , Khammo, N. , Secker, T. , McDonnell, G. and Keevil, C.W. (2010) Application of a fluorescent dual stain to assess decontamination of tissue protein and prion amyloid from surgical stainless steel during simulated washer-disinfector cycles. Journal of Hospital Infection , 75 , (1) , 66-71 . ( doi:10.1016/j.jhin.2009.12.023 ). (PMID:20303614).
• Giao, Maria S. , Azevedo, N.F. , Wilks, Sandra A. , Vieira, M.J. and Keevil, C.W. (2010) Effect of chlorine on incorporation of helicobacter pylori into drinking water biofilms. Applied and Environmental Microbiology , 76 , (5) , 1669-1673 . ( doi:10.1128/AEM.01378-09 ).
• Weaver, L. , Michels, H.T. and Keevil, C.W. (2010) Potential for preventing spread of fungi in air-conditioning systems constructed using copper instead of aluminium. Letters in Applied Microbiology , 50 , (1) , 18-23 . ( doi:10.1111/j.1472-765X.2009.02753.x ). (PMID:19943884 ).