Publications

Recent publications from the Environmental Healthcare Unit.

• Warnes, Sarah L., Summersgill, Emma N. and Keevil, C William (2015) Inactivation of murine norovirus on a range of copper alloy surfaces is accompanied by loss of capsid integrity. Applied and Environmental Microbiology, 81, (3), 1085-1091. (doi:10.1128/AEM.03280-14). (PMID:25452290).
• Phippen, Curtis William, Mikolajek, Halina, Schlaefli, Henry George, Keevil, Charles William, Webb, Jeremy Stephen and Tews, Ivo (2014) Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator. FEBS Letters (doi:10.1016/j.febslet.2014.11.002). (In Press).
• Meier, Alexandra, Tsaloglou, Nefeli-Maria, Mowlem, Matthew C., Keevil, C. William and Connelly, Douglas P. (2013) Hyperbaric biofilms on engineering surfaces formed in the deep sea. Biofouling, 29, (9), 1029-1042. (doi:10.1080/08927014.2013.824967).
• Warnes, Sarah L. and Keevil, C. William (2013) Inactivation of norovirus on dry copper alloy surfaces. PLoS ONE, 8, (9), 1-9. (doi:10.1371/journal.pone.0075017). (PMID:24040380).
Cerqueira, Laura, Fernandes, Ricardo M., Ferreira, Rui M., Oleastro, Mónica, Carneiro, Fátima, Brandao, Catarina, Pimentel-Nunes, Pedro, Dinis-Ribeiro, Mário, Figueiredo, Céu, Keevil, Charles W., Vieira, Maria J. and Azevedo, Nuno F. (2013) Validation of a PNA-FISH method for the detection of Helicobacter pylori clarithromycin resistance in gastric biopsies. Journal of Clinical Microbiology (doi:10.1128/JCM.00302-13). (PMID:23596234).
• Almeida, C., Azevedo, N. F., Bento, J. C., Cerca, N., Ramos, H., Vieira, M. J. and Keevil, C. W. (2013) Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH. European Journal of Clinical Microbiology & Infectious Diseases (doi:10.1007/s10096-012-1808-2).
• Warnes, S. L., Highmore, C. J. and Keevil, C. W. (2012) Horizontal transfer of antibiotic resistance genes on abiotic touch surfaces: implications for public health. mBio, 3, (6), e00489-[12 pages]. (doi:10.1128/mBio.00489-12).
• Gião, M.S. and Keevil, C.W. (2012) Hydrodynamic shear stress to remove Listeria monocytogenesbiofilms from stainless steel and polytetrafluoroethylene surfaces. Journal of Applied Microbiology, 114, (1), 256-265. (doi:10.1111/jam.12032).
• Azevedo, Nuno F., Bragança, Sofia M., Simões, Lúcia C., Cerqueira, Laura, Almeida, Carina, Keevil, Charles W. and Vieira, Maria J. (2012) Proposal for a method to estimate nutrient shock effects in bacteria. BMC Research Notes, 5, (1), 422. (doi:10.1186/1756-0500-5-422).
• Warnes, S.L., Caves, V. and Keevil, C.W. (2012) Mechanism of copper surface toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarization followed by slower rate of DNA destruction which differs from that observed for Gram-positive bacteria. Environmental Microbiology, 14, (7), 1730-1743. (doi:10.1111/j.1462-2920.2011.02677.x). (PMID:22176893).
• Warnes, S.L., Caves, V. and Keevil, C.W. (2012) Mechanism of copper surface toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarization followed by slower rate of DNA destruction which differs from that observed for Gram-positive bacteria. Environmental Microbiology, 14, (7), 1730-1743. (doi:10.1111/j.1462-2920.2011.02677.x). (PMID:22176893).
• Secker, T.J., Hervé, R., Zhao, Q., Borisenko, K.B., Abel, E.W. and Keevil, C.W. (2012) Doped diamond-like carbon coatings for surgical instruments reduce protein and prion-amyloid biofouling and improve subsequent cleaning. Biofouling, 28, (6), 563-569. (doi:10.1080/08927014.2012.698387). (PMID:22694725).
• Secker, T.J., Hervé, R. and Keevil, C.W. (2012) Letter to the editor. Response to the letter from M. Feckie. Journal of Hospital Infection, 80, (2), 181-182. (doi:10.1016/j.jhin.2011.11.006).
• Hervé, R. and Keevil, C.W. (2012) Current limitations about the cleaning of luminal endoscopes. Journal of Hospital Infection, 83, (1), 22-29. (doi:10.1016/j.jhin.2012.08.008). (PMID:23098682).
• Collins, Samuel L., Hervé, Rodolphe, Keevil, C.W., Blaydes, Jeremy P. and Webb, Jeremy S. (2011) Down-regulation of DNA mismatch repair enhances initiation and growth of neuroblastoma and brain tumour multicellular spheroids. PLoS ONE, 6, (12), e28123. (doi:10.1371/journal.pone.0028123). (PMID:22145025).
• Azevedo, N.F., Jardim, T., Almeida, C., Cerqueira, L., Almeida, A.J., Rodrigues, F., Keevil, C W. and Vieira, M.J. (2011) Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples. Antonie van Leeuwenhoek, 100, (3), 463-470. (doi:10.1007/s10482-011-9595-9). (PMID:21638111).
• Warnes, S.L. and Keevil, C.W. (2011) Mechanism of copper surface toxicity in vancomycin-resistant enterococci following wet or dry surface contact. Applied and Environmental Microbiology, 77, (17), 6049-6059. (doi:10.1128/AEM.00597-11). (PMID:21742916).
• Secker, T.J., Hervé, R. and Keevil, C.W. (2011) Adsorption of prion and tissue proteins to surgical stainless steel surfaces and the efficacy of decontamination following dry and wet storage conditions. Journal of Hospital Infection, 78, (4), 251-255. (doi:10.1016/j.jhin.2011.03.021). (PMID:21658801).
• Cerqueira, Laura, Fernandes, Ricardo M.., Ferreira, Rui M., Carneiro, Fátima, Dinis-Ribeiro, Mário, Figueiredo, Céu, Keevil, Charles W., Azevedo, Nuno F. and Vieira, Maria J. (2011) PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori. BMC Microbiology, 11, (1), 101-[7pp]. (doi:10.1186/1471-2180-11-101).
• Almeida, Carina, Azevedo, Nuno F., Santos, Sílvio, Keevil, Charles W. and Vieira, Maria J. (2011) Discriminating multi-species populations in biofilms with peptide nucleic acid fluorescence in situ hybridization (PNA FISH). PLoS ONE, 6, (3), e14786-[13pp]. (doi:10.1371/journal.pone.0014786).
• Gião, Maria S., Azevedo, Nuno F., Wilks, Sandra A., Vieira, Maria J. and Keevil, Charles W. (2011) Interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms. BMC Microbiology, 11, (1), 57-[10pp]. (doi:10.1186/1471-2180-11-57).
• Weaver, L., Noyce, J.O., Michels, H.T and Keevil, C.W. (2010) Potential action of copper surfaces on meticillin-resistant Staphylococcus aureus. Journal of Applied Microbiology, 109, (6), 2200-2205. (doi:10.1111/j.1365-2672.2010.04852.x). (PMID:21040269).
• Hervé, R., Secker, T.J. and Keevil, C.W. (2010) Current risk of iatrogenic Creutzfeld–Jakob disease in the UK: efficacy of available cleaning chemistries and reusability of neurosurgical instruments. Journal of Hospital Infection, 75, (4), 309-313. (doi:10.1016/j.jhin.2010.01.024). (PMID:20451298).
• Warnes, S.L., Gree, S.M., Michels, H.T. and Keevil, C.W. (2010) Biocidal efficacy of copper alloys against pathogenic enterococci involves degradation of genomic and plasmid DNAs. Applied and Environmental Microbiology, 76, (16), 5390-5401. (doi:10.1128/AEM.03050-09). (PMID:20581191).
• Almeida, C., Azevedo, Nuno F., Fernandes, R.M., Keevil, Charles W. and Vieira, M.J. (2010) A fluorescence in situ hybridization method using a peptide nucleic acid probe for the identification of Salmonella spp. in a broad spectrum of samples. Applied and Environmental Microbiology (doi:10.1128/AEM.01678-09).
• Howlin, R.P., Khammo, N., Secker, T., McDonnell, G. and Keevil, C.W. (2010) Application of a fluorescent dual stain to assess decontamination of tissue protein and prion amyloid from surgical stainless steel during simulated washer-disinfector cycles. Journal of Hospital Infection, 75, (1), 66-71. (doi:10.1016/j.jhin.2009.12.023). (PMID:20303614).
• Giao, Maria S., Azevedo, N.F., Wilks, Sandra A., Vieira, M.J. and Keevil, C.W. (2010) Effect of chlorine on incorporation of helicobacter pylori into drinking water biofilms. Applied and Environmental Microbiology, 76, (5), 1669-1673. (doi:10.1128/AEM.01378-09).
• Weaver, L., Michels, H.T. and Keevil, C.W. (2010) Potential for preventing spread of fungi in air-conditioning systems constructed using copper instead of aluminium. Letters in Applied Microbiology, 50, (1), 18-23. (doi:10.1111/j.1472-765X.2009.02753.x). (PMID:19943884 ).