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The University of Southampton
EMBRN/COST International Mast Cell and Basophil Meeting

Programme

Programme

Wednesday, 23rd November 2011

17.00 – 21.00 Registration desk open

Poster set up


Thursday, 24th November 2011

8.00 – 9.00 Registration

Tea and coffee with pastries

Poster set up

9.00 – 9.30 Welcome

Andrew F Walls, Southampton, President of Southampton Meeting

Marcus Maurer, Berlin, Chair of COST Action

Stephan C Bischoff, Stuttgart, President of EMBRN

Session A: Highlights in mast cell and basophil research I

Chairs: Gunnar Pejler, Uppsala and Marcus Maurer, Berlin

9.30 – 10.00 Rick L Stevens, Boston: Importance of mast cell-restricted proteases in innate immunity, inflammation, and blood coagulation (O1)

10.00 - 10.30 Jean S. Marshall, Halifax: Models of viral infection and cancer reveal novel roles for mast cells in effector cell recruitment (O2)

10.30 – 10.50 Gunnar Nilsson, Stockholm: Increased vascular permeability by mast cells mediated by heparin-initiated bradykinin formation (O3)

10.50 – 11.20 Tea and coffee with biscuits

Session B: Work Group 1 Symposium: Physiological and pathophysiological importance of mast cells and basophils in health and disease.

Insights into mast cell and basophil signal transduction pathways

Chairs: Francesca Levi-Schaffer, Jerusalem and Nicolas Charles, Paris

11.20 – 11.40 Petr Draber, Prague: STIM1-cytoskeleton cross-talk in mast cell movement and degranulation (O4)

11.40 – 12.00 Didier Ebo, Antwerp: Flow-assisted analysis of in vitro activated basophils (O5)

12.00 – 12.20 Araceli Tobio Ageitos, Santiago de Compostela: C-kit mutations and PKC crosstalk: PKC translocates to nucleus only in HMC-1560,816 cells (O6)

12.20 – 12.30 Thomas A Brown, Southampton: Mast Cell Carboxypeptidase: A Marker for the Diagnosis of Drug-Induced Allergic Reactions and for Predicting which Patients are at Risk (O7)

12.30 – 12.40 Jolien Suurmond, Leiden: Toll-Like Receptor triggering can synergize with IgE-mediated activation of human mast cells in autoimmunity (O8)

12.40 – 12.50 Matthias Mack, Regensburg: Basophils control T cell responses and experimental colitis in mice (O9)

12.50 – 13.50 Buffet lunch


Session C: Highlights in mast cell and basophil research II

Chairs: Peter Bradding, Leicester and Per Stahl Skov, Copenhagen
13.50 – 14.20 Hirohisa Saito, Tokyo: The mast cell and basophil transcriptome (O10)

Session D: Work Group 2 Symposium: Methodological approaches for the investigation of mast cell and basophil biology

Chairs: Franco H Falcone, Nottingham and Karin Hartmann, Cologne

14.20 – 14.40 Axel Roers, Dresden: Novel mouse models for the investigation of mast cell in vivo functions (O11)

14.40 – 15.00 Franco H. Falcone, Nottingham: Purification of peripheral blood basophils from human blood (O12)

15.00 – 15.20 Vadim Sumbayev, Chatham Maritime: Transfection of Hematopoietic Cells of Myeloid Lineage: Current Achievements and Future Perspectives (O13)

15.20 – 15.30 Ahmed Fawzy, Southampton/Cairo: Development and Characterisation of BB5, a New Basophil-Specific Monoclonal Antibody that Binds to Basogranulin (O14)

15.30 – 15.40 Lucie Potuckova, Prague: Functionalized gold nanoparticle-based immuno-PCR, a new method for sensitive detection of cytokines produced or utilized by mast cells (O15)

15.40 – 15.50 Hans-Jürgen Hoffmann, Aarhus: Basophil Sensitivity Decreases in Parallel with Clinical Sensitivity in Allergen Challenge during Subcutaneous Immunotherapy (SCIT) in Subjects Allergic to Grass Pollen (O16)

15.50 – 16.20 Tea and coffee with cake

15.50 – 17.10 Poster Viewing: All poster presenters present
Posters listed on page 11

Work Group 1 : Poster walk by Frank Redegeld, Utrecht and Ronit Sagi-Eisenberg, Tel Aviv

Work Group 2: Poster walk by Franco Falcone, Nottingham

Work Group 3: Poster walk by Bernhard Gibbs, Chatham Maritime and Jenny Hallgren, Uppsala

Work Group 4: Poster walk by Tilo Biedermann, Tübingen and Jane Warner, Southampton

17.10 – 17.40 Separate Meetings for COST Work Groups 1, 2, 3 and 4 (Main Meeting Room)

17.40 – 18.40 Joint business meeting of COST working groups
COST Management Committee Meeting

19.00 Social Event – Buses leave for HMS Warrior

Friday, 25th November 2011

7.30 – 8.30 Board of Directors of EMBRN and COST Management Committee Meeting (Allertons Room, situated off the Main Restaurant, Grand Harbour Hotel)

8.30 – 9.00 Tea and coffee with pastries

Session E: Highlights in mast cell and basophil research III
Chairs: Carlo Pucillo, Udine and Bernhard Gibbs, Chatham Maritime

9.00 – 9.30 Hajime Karasuyama, Tokyo. Emerging roles of basophils in protective and pathological immune responses (O17)

Session F: Work Group 3 Symposium: Identification of biological and pharmaceutical mast cell and basophil related targets

Chairs: Bernhard Gibbs, Chatham Maritime and Jenny Hallgren, Uppsala

9.30 – 9.50 Nicolas Charles, Paris: Basophils and Autoimmunity: Making sense of IgE in lupus (O18)

9.50 – 10.10 Peter Peachell, Sheffield: Human lung mast cell releasability: role of syk (O19)

10.10 – 10.30 Silvia Bulfone-Paus, Borstel: The role of coronins in the control of mast cell activation (O20)

10.30 – 10.40 Gianni García-Faroldi, Uppsala: ADAMTS: novel proteases expressed by activated mast cells (O21)

10.40 – 10.50 Axel Lorentz, Stuttgart: Complexin II is involved in enhanced exocytosis of human intestinal mast cells following IL-4 priming (O22)

10.50 – 11.00 Ian Ashmole, Leicester: CRACM/Orai ion channel expression and function in human lung mast cells (O23)

11.00 – 11.30 Tea and coffee with biscuits

Session G: Mast cells as Drug Targets

Chairs: Martin Church, Southampton/Berlin and Frank Siebenhaar, Berlin

11.30 – 11.50 Alan Edwards, Isle of Wight: Landmarks in the development of drugs that target mast cells (O24)

11.50 - 12.00 Anja Rabenhorst, Cologne: Mast cells play a pro-tumorigenic role in primary cutaneous lymphoma (O25)

12.00 - 12.10 Luis Escribano, Toledo: Impact of KIT mutation in the evolution of serum basal tryptase levels in indolent systemic mastocytosis. (O26)

12.10 – 12.20 Elizabeth Doyle, Berlin: Tryptase therapy counteracts lethal effects of snake venom: a new treatment for snakebite (O27)

12.20 – 12.30 Giorgia Gri, Udine: AhR stimulation modulates mast cell degranulation and regulatory cytokine response (O28)

12.30 – 12.40 Paul Forsythe, Hamilton: A Pseudomonas aeruginosa derived acyl homoserine lactone promotes and inhibits mast cell responses to stimuli (O29)

12.40 – 12.50 Joakim S Dahlin, Uppsala: CD11c+ cells are required for mast cell progenitor recruitment to lung in a mouse model of allergic airway inflammation (O30)

12.50 – 14.00 Buffet lunch

Session H: Work Group 4 Symposium: Therapeutic potential of mast cell and basophil targeting strategies

Chairs: Stephan C Bischoff, Stuttgart and Frank Redegeld, Utrecht

14.00 – 14.20 Sven-Erik Dahlen, Stockholm: New insight into antagonism of mast cell-dependent responses (O31)

14.20 – 14.40 Stephen T Holgate, Southampton: Stratified approaches to mast cell related therapeutics for allergic diseases (O32)

14.40 – 15.00 Clemens A Dahinden, Bern: Mast cell and basophil genechip analyses and consequences for the understanding and management of disease (O33)

15.00 – 15.10 Lars Hellman, Uppsala: Vaccination against IL-33 effectively reduces dermatitis scores on mite allergen induced dermatitis in NC/Nga mice (O34)

15.10 – 15.20 Mattias Enoksson, Stockholm: Intraperitoneal influx of neutrophils in response to IL-33 is mast cell dependent (O35)

15.20 – 15.30 Xiaoying Zhou, Southampton: Anaphylaxis is Associated with Increased Serum Levels of Mast Cell Chymase (O36)

15.30 - 16.15 EMBRN General Assembly and Closure of Meeting

15.30 Tea and coffee with cake available

Poster Presentations

Work Group 1

Andrea Dichlberger, Helsinki: Arachidonic acid-induced formation of human mast cell lipid bodies is mediated by long-chain acyl-CoA synthetases (P1)

Vadim V. Sumbayev, Chatham Maritime: The Role of Hypoxia-Inducible Factor 1 and Associated Pathways in Host Innate Immune Defence, Allergic Inflammation, and Leukaemia Progression (P2)

Bernhard F. Gibbs, Chatham Maritime: Regulation of Human Mast Cell and Basophil Responses by Hypoxia-Inducible Factor-1α. (P3)

Cecilia K Andersson, Lund: Alveolar mast cell expression of the high-affinity IgE receptor differs between allergic asthma and rhinitis (P4)

Yu Fang, Gothenburg: Enhancing the effectiveness of vaccine adjuvants by targeting mast cells (P5)

Thomas Hügle, Basel: Mast Cell Fibrogenesis: Direct Vesicle Transmission by Mast Cells to Fibroblasts and Lymphocytes in Systemic Sclerosis (P6)

Qingjun Pan, Zhanjiang: Number and phenotype of circulating basophils for monitoring disease activity in systemic lupus erythematosus (P7)

Helena Horáková, Prague: Regulatory role of the protein 4.1R in mast cell signaling and chemotaxis (P8)

Rohit Saluja, Stockholm: FcεR1-mediated mast cell reactivity is amplified through prolonged Toll-like receptor-ligand treatment (P9)

Walid H Beghdadi, Paris: Mast cells decrease renal fibrosis in unilateral ureteral obstruction by a murine mast-cell chymase (mMCP4)-dependent mechanism (P10)

Emily J. Swindle, Southampton: Suppression of Mast Cell Activation Following Co-culture With Differentiated Bronchial Epithelial Cells (P11)

Rosa Torres, Barcelona:House dust mite aeroallergens can induce airway pathology in C57BL/6 by means of a mast cell-independent mechanism (P12)

Ulrich Blank, Paris: Critical role of the neutrophil-associated high affinity receptor for IgE in the pathogenesis of experimental cerebral malaria (P13)

Julia A Trosien, Berlin: Anti-inflammatory effects of a polyglycerolsulfate on type I allergic responses (P14)

Ana Claudia Zenclussen, Magdeburg: Mast cells are critical for pregnancy establishment and maintenance (P15)

Federico Jensen, Magdeburg: Female sex hormones induce the migration of mast cells to the uterus and provoke their degranulation (P16)

Nashwan Asmail, Uppsala: Mast cells as regulators of tumor angiogenesis and progression (P17)

Ananya Roy, Uppsala: Serglycin limits enteropathy in Trichinella spiralis-infected mice (P18)

Laura Sánchez-Muñoz, Toledo: Serum carboxypeptidase is a predictive factor of symptoms in children with mastocytosis in the skin (P19)

Xiaoying Zhou, Southampton: Mast Cell Chymase Can Induce Disruption of the Bronchial Epithelium and Loss of Adhesion Molecule Expression (P20)

Osama Sawesi, Uppsala: Serglycin contributes to cytokine secretion during Toxoplasma gondii infection (P21)

Mogib El-Rahman Khedr, Southampton: Pro-Inflammatory Actions of Human Mast Cell Tryptase in Mice Deficient in Protease Activated Receptor 2 (PAR2) (P22)

Anna James, Stockholm Sweden: Corticosteroid treatment selectively decreases mast cells in the smooth muscle and epithelium of asthmatic bronchi (P23)

Hilary S Whitworth, Southampton: Dipeptidyl Peptidase I: A novel marker of Allergic Reactions to Food (P24)

Michael Thorpe, Uppsala: The extended cleavage specificity of the mast cell chymase of the crab eating macaque (Macaca fascicularis) - an interesting animal model for the analysis of the in vivo function of the human mast cell chymase (P25)

Ida Waern, Uppsala: Mast cell chymase is a negative regulator of allergic sensitization in house dust mite induced airway inflammation (P26)

Jani Lappalainen, Helsinki: Viral RNA elicits an interferon response in mast cells (P27)

Work Group 2

Nurit Pereg-Azouz, Tel Aviv University: Functional Genomics Analysis of Mast Cell Exocytosis Reveals Isoform Specific Regulation by Rab GTPases (P28)

Vladimir A. Gimenez-Rivera, Berlin: Mast cells control chronic type IV allergic skin inflammation in mice (P29)

Angel Vodenicharov, Stara Zagora: Mast cells in porcine kidney interlobar arteries – a TEM study (P30)

Ralph Rühl, Debrecen: Lipidomic approach for analysis of eicosanoids and docosanoids in serum and PBMCs of atopic dermatitis patients (P31)

Marcus Maurer, Berlin: Establishment of a Bradykinin Skin Test as a Tool for Monitoring Pharmacologic Effects (P32)

Doina Mihaila, Iasi: Is there a pattern of mastocytes distribution within inflammatory diseases and tumoral processes? Observations upon a few cases (P33)

Chatterjea, D, St. Paul: Mast cell degranulation mediates compound 48/80-induced plantar thermal hypernociception and the local inflammatory response in mice (P34)

Anke Petzold, Dresden: Local clonal expansion of bone marrow-derived mast cell progenitors recruited into inflamed skin (P35)

Work Group 3

Ambili Menon, Karlsruhe: PEST–domain enriched tyrosine phosphatase is a positive regulator of anaphylaxis in mice (P36)

Catherine Vial, Leicester: Characterisation of the human mast cell line HMC-1 ectonucleotidase activity (P37)

Anton de Groot, Leiden: An adenoviral knock-down screen in primary human mast cells reveals novel targets for therapy in allergic rhinitis (P38)

Barbara Frossi, Udine: OX40L-engagement interferes with IgE/Ag-driven mast cell activation by selectively acting on Fyn, PI3K and RhoA (P39)

Fabio R Melo, Uppsala: Serglycin regulates apoptotic versus necrotic cell death in mast cells (P40)

Ulrich Blank, Paris: Munc18-2 controls granule translocation in mast cells through dynamic interactions with the fusion machinery and the microtubule cytoskeleton (P41)

Sabine Altrichter, Berlin: IgE mediated autoallergy against thyroid peroxidase – a novel pathomechanism of chronic spontaneous urticaria? (P42)

Anja Förster, Cologne: Differential regulation of apoptosis induced by anti-Fas/CD95 and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in primary and neoplastic basophils: potential implications for treatment of basophilia associated with chronic myeloid leukemia and other hematologic malignancies (P43)

Erola Ainsua-Enrich, Barcelona: The adaptor 3BP2 is required for optimal high affinity IgE receptor signaling in human mast cells (P44)

Elin Rönnberg, Uppsala: Mast cells express tyrosine hydroxylase and store dopamine in a serglycin-dependent manner (P45)

Anders Lundequist, Uppsala: Differential regulation of nuclear receptor 4a subfamily following mast cell activation (P46)

Brice Korkmaz, Tours: Discriminating between the activities of human cathepsin G and chymase using fluorogenic substrates (P47)

Paulo De Sepulveda, Marseille: KIT-D816V oncogenic activity is maintained under control by the juxtamembrane docking site YY568-570 (P48)

Paulo De Sepulveda, Marseille: Mutant KIT receptor as a model to identify novel targets in mast cells (P49)

Work Group 4

Anca Chiriac, Iaşi: Transient disappearance of urticaria pigmentosa lesions during Enoxaparinum treatment: A case report (P50)

Daniele De Filippis, Naples: Palmitoylethanolamide ameliorates contact allergic dermatitis in mice: involvement of mast cells and endocannabinoids (P51)

Krisztina V. Vukman, Dublin: Tegumental antigens of Fasciola hepatica (FhTeg) suppress inflammatory immune response in mast cells (P52)

AB Penissi, Mendoza: Phenolic molecules from olive oil inhibit rat peritoneal mast cell activation (P53)

Laila Karra, Jerusalem: Dexamethasone influences CD300a inhibitory receptor function on human and murine mast cells (P54)

Jane Spirkoski, Uppsala: Mast cells are sensitive to apoptosis induced by the sigma 2 receptor agonist siramesine (P55)

Jane A Warner, Southampton: Mast cells in the proximal and distal airways: Effects of steroids (P56)

Laurie CK Lau, Southampton: Inhibition of mast cell mediator release in the presence of honey (P57)

Marc Freichel, Heidelberg: Regulation of mast cell activation by TRP protein-mediated Ca2+ entry (P58)

Tilo Biedermann, Tübingen: Co-factor dependent anaphylaxis triggered by innate immune signals is mediated by basophils (P59)

Ivana Hálová, Prague: Involvement of tetraspanin CD9 in mast cell signaling and chemotaxis (P60)

Rick Stevens
Hajime Karasuyama
Jean Marshall
Hirohisa Saito
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