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The University of Southampton
Institute for Life Sciences

Bioengineering PGR seminar  Event

15:00 - 16:00
22 November 2017

For more information regarding this event, please email Dr Rahul S Tare at .

Event details

Improving fracture outcome using nanoparticle-mediated drug delivery Bioengineering Seminar with speaker Alethia Hailes.

Abstract: Pharmaceutical treatments to aid bone fracture repair are not yet available in clinic, despite their potential advantage as a replacement or adjunct to invasive and costly surgery. Potential therapies may target signaling pathways involved in fracture repair, but are limited by off-target effects and poor bioavailability at the injury site. Polymeric nanoparticles (polymersomes; PMs) may provide a solution by enabling controlled spatio-temporal drug delivery. In this study we tested the hypothesis that PMs passively accumulate at bone fracture sites in a mouse model when injected at specific timepoints post-injury. In a pilot experiment, 1mm drill-hole defects were created in the femurs of female MF1 mice under general anesthesia. The mice were separated into two groups, and administered with a 200μl IV injection of PMs loaded with fluorescent dye, DiR, either 1 hour or 7 days post-surgery. Controls at both time points received no injection. 48hrs post-injection the whole body and extracted organs were imaged using the in vivo imaging system (IVIS, PerkinElmer) and fluorescence intensity was quantified. PM-payload fluorescence was observed at the site of injury in both treatment groups, with a 4-fold higher intensity when injected 1 hour compared to 7 days post-surgery. A strong signal observed in the liver decreased by a factor of 1.3 between the two groups. Further experiments are necessary to determine statistical significance. Our results show passive accumulation of PMs at fracture sites during different phases of healing; identifying that PMs may be a suitable method of spatio-temporally controlling drug delivery during fracture healing.

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