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The University of Southampton
Institute for Life Sciences

IfLS Imaging Seminar: Multi-modality, high-throughput optical nanoscopy using integrated photonic circuits Event

Image: Dr Balpreet Singh Ahluwalia
12:00 - 13:00
20 November 2018
Room 2207, Life Sciences Building (85), Highfield Campus

Event details

Associate Professor Balpreet Singh Ahluwalia, UiT – The Arctic University of Norway

All welcome

Seminar Title: Multi-modality, high-throughput optical nanoscopy using integrated photonic circuits

Abstract: Super-resolution optical microscopy commonly referred to as optical nanoscopy has provided a glimpse of its future impact on biology, life science and medical diagnostics [1, 2]. Although optical nanoscopy offers unprecedented opportunities, the vision to replace present day standard microscopes with optical nanoscopes is still far from reality due to present limitations of nanoscopy methodologies. The widespread adoption of nanoscopy is currently hindered by system complexity, cost, lack of multi-modality, imaging speed and the need for skilful and highly trained operators.

Photonic integrated circuits (PIC) reduce the footprint, cost and complexity of optical systems. PIC technology enables on-chip integration of several optical functions. The compatibility with standard optical fibre components enables high-speed light coupling into PICs. Here, I will provide an overview of several optical nanoscopy methodologies developed that we have developed using an integrated photonic-chip. We have developed photonic-chip based single molecule localization optical microscopy (SML-OM), structured illumination microscopy (SIM), light intensity fluctuation based optical nanoscopy (e.g. ESI, SRRF, MUSICAL) and correlative light-electron microscopy. Using the principle of single molecule localization, we demonstrate a resolution of 50 nm using chip-based nanoscopy [3]. Furthermore, we demonstrate the capability of chip-based nanoscopy to acquire super-resolved images over millimetre field-of-view scale; a 100-fold increase in imaging area as compared to other nanoscopy solutions, thus opening the opportunities of high-throughput optical nanoscopy [3].

In this talk, I will also provide a short overview of different biological applications that focus on performing high-speed, live cell 3D nanoscopy of sub-cellular organelles such as mitochondria, endosomes, vesicle and nano-pores present in cell membrane. 

1. Lothar S,, “A guide to super-resolution fluorescence microscopy”, J. Cell Biol, 190, 165, 2010.
2. S. W. Hell, “Far-Field Optical Nanoscopy”, Science 316: 1153-1158 (2007).
3. R. Diekmann, O. I. Helle, C. I. Oie, P. McCourt, T. R. Huser, M. Schuttpelz, and B. S. Ahluwalia, “Chip-based wide field-of-view nanoscopy,” Nat Photonics 11, 322 (2017).

Biography:  Ahluwalia has PhD in Electrical Engineering (major in photonics) in 2007 from Nanyang Technological University, Singapore. He is working as an Associate Professor at the Department of Physics and Technology, UiT The Arctic University of Norway. He leads a cross-disciplinary optical nanoscopy research group that focus towards the development of multi-modality and high-throughput optical nanoscopy and its biological application in several physiologically and clinically relevant bio-applications such as molecular cancer research, vascular biology, perinatology and pathology. Currently, he is on a sabbatical working at the Zepler Institute, University of Southampton till Aug 2019.

Ahluwalia, is Group Leader of Ultrasound, Microwave and Optics Group at the department. During last few years, the UMO group has attracted 4 ERC projects (3 ERC St. Grant, 1 ERC PoC), several other EU projects (MSCA-ITN, MSA-IF) and Norwegian Research Council funded projects (FRIPRO, FORNY, BioTek2021, Nano2021, etc)

Speaker information

Associate Professor Balpreet Singh Ahluwalia,UiT – The Arctic University of Norway,Group Leader (Ultrasound, Microwave & Optics), Department of Physics and Technology

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