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Computationally Intensive Imaging

Dr Andrew Shevchuk PhD

Senior Research Fellow, Institute for Life Sciences and Research Career Track Lecturer, Optoelectronics Research Centre

Dr Andrew Shevchuk's photo

Dr Andrew Shevchuk is part of the Institute for Life Sciences at the University of Southampton.

Multifunctional scanning probe microscopy for bio applications.

2000 - M.Sc. in Computer Science. Dept. of specialized computer systems, Faculty of Applied Mathematics, Kiev Polytechnic Institute, National Technical University of Ukraine

2003 - Ph.D. in Biophysics, Medical Physics and Bioengineering. Faculty of Medicine, Imperial College London

2003 - 2012 Post-doctoral Research Fellow, Imperial College London

Research interests

My research is primarily focused on the design, development and application of Ion Conductance Microscopy in combination with other scanning probe and live imaging techniques to a wide range of biological studies. I introduce latest technological advances to ion conductance microscopy and push its performance to the limits aiming to create powerful and versatile technique that can answer questions currently unaddressable by other methods. Recent improvements in resolving power and imaging rate have played a pivotal role and made it possible to directly image endocytosis in living cells. This now opens a possibility for detailed studies of such important process as receptor cycling, drug delivery and infection.

Self-funded students (full-time PhD only) are welcome if they are interested in either Scanning Probe Microscopy (SICM, SECM), programming of control systems, electrochemistry, biophysics, live cell imaging, cell membrane transport, cell and molecular biology.

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1.   Shevchuk, A.I., P.Novak, M.Taylor, I.A.Diakonov, A.Ziyadeh-Isleem, M.Bitoun, P.Guicheney, M.J.Lab, J.Gorelik, C.J.Merrifield, D.Klenerman, and Y.E.Korchev. 2012. An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy. Journal of Cell Biology 197:499-508.

2.   Miragoli, M., A.Moshkov, P.Novak, A.Shevchuk, V.O.Nikolaev, I.El Hamamsy, C.M.F.Potter, P.Wright, S.H.S.A.Kadir, A.R.Lyon, J.A.Mitchell, A.H.Chester, D.Klenerman, M.J.Lab, Y.E.Korchev, S.E.Harding, and J.Gorelik. 2011. Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells. Journal of the Royal Society Interface 8:913-925.

3.   Shevchuk, A.I., P.Novak, Y.Takahashi, R.Clarke, M.Miragoli, B.Babakinejad, J.Gorelik, Y.E.Korchev, and D.Klenerman. 2011. Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe. Nanomedicine 6:565-575.

4.   Takahashi, Y., A.I.Shevchuk, P.Novak, Y.J.Zhang, N.Ebejer, J.V.Macpherson, P.R.Unwin, A.J.Pollard, D.Roy, C.A.Clifford, H.Shiku, T.Matsue, D.Klenerman, and Y.E.Korchev. 2011. Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces. Angewandte Chemie-International Edition 50:9638-9642.

5.   Adler, J., A.I.Shevchuk, P.Novak, Y.E.Korchev, and I.Parmryd. 2010. Plasma membrane topography and interpretation of single-particle tracks. Nature Methods 7:170-171.

6.   Takahashi, Y., A.I.Shevchuk, P.Novak, Y.Murakami, H.Shiku, Y.E.Korchev, and T.Matsue. 2010. Simultaneous Noncontact Topography and Electrochemical Imaging by SECM/SICM Featuring Ion Current Feedback Regulation. Journal of the American Chemical Society 132:10118-10126.

7.   Novak, P., C.Li, A.I.Shevchuk, R.Stepanyan, M.Caldwell, S.Hughes, T.G.Smart, J.Gorelik, V.P.Ostanin, M.J.Lab, G.W.J.Moss, G.I.Frolenkov, D.Klenerman, and Y.E.Korchev. 2009. Nanoscale live-cell imaging using hopping probe ion conductance microscopy. Nature Methods 6:279-281.

8.   Dutta, A.K., Y.E.Korchev, A.I.Shevchuk, S.Hayashi, Y.Okada, and R.Z.Sabirovf. 2008. Spatial distribution of maxi-anion channel on cardiomyocytes detected by smart-patch technique. Biophysical Journal 94:1646-1655.

9.   Li, C., N.Johnson, V.Ostanin, A.Shevchuk, L.M.Ying, Y.Korchev, and D.Klenerman. 2008. High resolution imaging using scanning ion conductance microscopy with improved distance feedback control. Progress in Natural Science-Materials International 18:671-677.

10.   Shevchuk, A.I., P.Hobson, M.J.Lab, D.Klenerman, N.Krauzewicz, and Y.E.Korchev. 2008. Endocytic pathways: combined scanning ion conductance and surface confocal microscopy study. Pflugers Archiv-European Journal of Physiology 456:227-235.

11.   Shevchuk, A.I., P.Hobson, M.J.Lab, D.Klenerman, N.Krauzewicz, and Y.E.Korchev. 2008. Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy. Biophysical Journal 94:4089-4094.

12.   Korchev, Y., D.Sanchez, P.Anand, J.Gorelik, Y.J.Zhang, A.Shevchuk, I.Vodyanoy, M.Lab, and D.Klenerman. 2007. "Non-contact" mechanical characterization and stimulation of cells using scanning ion conductance microscopy. Biophysical Journal 33A.

13.   Gorelik, J., N.N.Ali, A.I.Shevchuk, M.Lab, C.Williamson, S.E.Harding, and Y.E.Korchev. 2006. Functional characterization of embryonic stem cell-derived cardiomyocytes using scanning ion conductance microscopy. Tissue Engineering 12:657-664.

14.   Shevchuk, A.I., G.I.Frolenkov, D.Sanchez, P.S.James, N.Freedman, M.J.Lab, R.Jones, D.Klenerman, and Y.E.Korchev. 2006. Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy. Angewandte Chemie-International Edition 45:2212-2216.

15.   Gorelik, J., Y.J.Zhang, D.Sanchez, A.Shevchuk, G.Frolenkov, M.Lab, D.Klenerman, C.Edwards, and Y.Korchev. 2005. Aldosterone acts via an ATP autocrine/paracrine system: The Edelman ATP hypothesis revisited. Proceedings of the National Academy of Sciences of the United States of America 102:15000-15005.

16.   Zhang, Y.J., J.Gorelik, D.Sanchez, A.Shevchuk, M.Lab, I.Vodyanoy, D.Klenerman, C.Edwards, and Y.Korchev. 2005. Scanning ion conductance microscopy reveals how a functional renal epithelial monolayer maintains its integrity. Kidney International 68:1071-1077.

17.   Gorelik, J., Y.J.Zhang, A.I.Shevchuk, G.I.Frolenkov, D.Sanchez, M.J.Lab, I.Vodyanoy, C.R.W.Edwards, D.Klenerman, and Y.E.Korchev. 2004. The use of scanning ion conductance microscopy to image A6 cells. Molecular and Cellular Endocrinology 217:101-108.

18.   Gorelik, J., A.Shevchuk, M.de Swiet, M.Lab, Y.Korchev, and C.Williamson. 2004. Comparison of the arrhythmogenic effects of tauro- and glycoconjugates of cholic acid in an in vitro study of rat cardiomyocytes. Bjog-An International Journal of Obstetrics and Gynaecology 111:867-870.

19.   Gorelik, J., A.I.Shevchuk, G.I.Frolenkov, I.A.Diakonov, M.J.Lab, C.J.Kros, G.P.Richardson, I.Vodyanoy, C.R.W.Edwards, D.Klenerman, and Y.E.Korchev. 2003. Dynamic assembly of surface structures in living cells. Proceedings of the National Academy of Sciences of the United States of America 100:5819-5822.

20.   Gorelik, J., A.I.Shevchuk, I.Diakonov, M.de Swiet, M.Lab, Y.Korchev, and C.Williamson. 2003. Dexamethasone and ursodeoxycholic acid protect against the arrhythmogenic effect of taurocholate in an in vitro study of rat cardiomyocytes. Bjog-An International Journal of Obstetrics and Gynaecology 110:467-474.

21.   Gorelik, J., I.Vodyanoy, A.I.Shevchuk, I.A.Diakonov, M.J.Lab, and Y.E.Korchev. 2003. Esmolol is antiarrhythmic in doxorubicin-induced arrhythmia in cultured cardiomyocytes - determination by novel rapid cardiomyocyte assay. Febs Letters 548:74-78.

22.   Bruckbauer, A., L.M.Ying, A.M.Rothery, D.J.Zhou, A.I.Shevchuk, C.Abell, Y.E.Korchev, and D.Klenerman. 2002. Writing with DNA and protein using a nanopipet for controlled delivery. Journal of the American Chemical Society 124:8810-8811.

23.   Gorelik, J., S.E.Harding, A.I.Shevchuk, D.Koralage, M.Lab, M.de Swiet, Y.Korchev, and C.Williamson. 2002. Taurocholate induces changes in rat cardiomyocyte contraction and calcium dynamics. Clinical Science 103:191-200.

24.   Gorelik, J., A.Shevchuk, M.Ramalho, M.Elliott, C.Lei, C.F.Higgins, M.J.Lab, D.Klenerman, N.Krauzewicz, and Y.Korchev. 2002. Scanning surface confocal microscopy for simultaneous topographical and fluorescence imaging: Application to single virus-like particle entry into a cell. Proceedings of the National Academy of Sciences of the United States of America 99:16018-16023.

25.   Gorelik, J., Y.C.Gu, H.A.Spohr, A.I.Shevchuk, M.J.Lab, S.E.Harding, C.R.W.Edwards, M.Whitaker, G.W.J.Moss, D.C.H.Benton, D.Sanchez, A.Darszon, I.Vodyanoy, D.Klenerman, and Y.E.Korchev. 2002. Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system. Biophysical Journal 83:3296-3303.

26.   Gu, Y.C., J.Gorelik, H.A.Spohr, A.Shevchuk, M.J.Lab, S.E.Harding, I.Vodyanoy, D.Klenerman, and Y.E.Korchev. 2002. High-resolution scanning patch-clamp: new insights into cell function. Faseb Journal 16:748.

27.   Shevchuk, A.I., J.Gorelik, S.E.Harding, M.J.Lab, D.Klenerman, and Y.E.Korchev. 2001. Simultaneous measurement of Ca2+ and cellular dynamics: Combined scanning ion conductance and optical microscopy to study contracting cardiac myocytes. Biophysical Journal 81:1759-1764.

Dr Andrew Shevchuk
Institute for Life Sciences University of Southampton 3041, Life Sciences Building 85 Highfield Southampton SO17 1BJ (023) 8059 4288
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