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The University of Southampton
Computationally Intensive Imaging

Imaging Seminar Series Event

Origin: 
Airy light beam illuminating cells
Time:
16:00
Date:
2 April 2015
Venue:
Room 2207 Building 85 Highfield Campus

For more information regarding this event, please telephone Dr Sumeet Mahajan on 023 8059 3591 or email S.Mahajan@soton.ac.uk .

Event details

Accelerating progress in Biomedical Imaging and Manipulation

The last decade has seen immense advances in photonics based methods for imaging and manipulation. However in many photonics fields there is a recognition that using the direct form of a laser output – the Gaussian beam – is restrictive for a number of applications in biophotonics including manipulation, imaging and beyond [1]. Professor Dholakia will describe the use of shaped light fields including propagation invariant (‘non-diffracting’) light fields and complex beam shaping. Propagation invariant light fields, as the name suggests retain their transverse intensity profile upon propagation. Bessel light fields and Airy light fields are prime examples of such beams. They can used for cell transfection, optical coherence tomography and moving trapped particles.

In terms of imaging, single plane illumination (light sheet) microscopy (SPIM) offers a myriad of unique advantages. Orthogonal detection allows rapid imaging of large, three-dimensional, samples of living tissue. Illumination with a thin sheet of light ensures high contrast by minimizing the fluorescent background. Moreover, by restricting the sample exposure to a single plane, photo-bleaching and damage are minimized. This is crucial when imaging photo-sensitive samples over a larger period of time. Recent enhancements to the original technique attempt to overcome the inherent trade off between axial resolution and field-of-view of conventional light sheet microscopy. Until recently, this was only achieved by compromising on one or more of its key advantages: high contrast, time-resolution, or minimal sample exposure. Professor Dholakia will discuss the use of propagation invariant light fields for the enhancement within this imaging modality [2-4].

 

References

[1] K Dholakia & T. Čižmár, “Shaping the future of manipulation”. Nature Photonics 5, 335 (2011)

[2] T.A. Planchon et al. “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination” Nature Methods 8, 417 (2011)

[3] T. Vettenburg et al. “Light sheet microscopy using an Airy beam”, Nature Methods 11, 541 (2014)

[4] Zhengyi Yang, Martynas Prokopas, Jonathan Nylk, Clara Coll-Llado, Frank J. Gunn-Moore, David E.K.Ferrier, Tom Vettenburg and Kishan Dholakia: “A compact Airy beam light sheet microscope with a tilted cylindrical lens”, Biomedical Optics Express, 10, (5), 3435 (2014)

Speaker information

Professor Kishan Dholakia,School of Physics and Astronomy,University of St Andrews

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