The microscopy core facility provides a specialist microscopy service to the Unit as well as providing training and advice to staff and students in microscopy techniques.
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All the resources necessary to carry out immunohistochemistry and immunofluorescence microscopy studies on frozen tissue or cell cultures as well as post-embedding processing of paraffin embedded tissue are provided.
Services provided
- Freezing tissue samples and cryosectioning.
- Immunolabelling of tissue sections or cells for bright field, fluorescence and confocal imaging.
- Image collection and analysis.
- Preparation of figures to publication standard.
- Training in microscopy techniques, advising on the planning of experiments and assisting with image collection and analysis.
- Providing information on the facilities available within the Faculty and helping with the planning of microscopy related projects.
- The facility enjoys a close working relationship with the Biomedical Imaging Unit (BIU) and Histochemistry Research Unit (HRU).
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Equipment provided
Microscope
Olympus CKX41 inverted microscope for bright-field, phase contrast and fluorescence imaging complete with digital camera and PC to enable straightforward image acquisition of cells in culture or cells/tissues labelled for light microscopy. The microscope is equipped with blue, red and green filters allowing the collection of UV (eg Dapi), green (eg FITC) and red (eg TRITC) fluorescence, respectively. Currently the microscope is equipped with x4, x10, x10 (phase contrast) and x40 objective lenses. A x20 fluorite lens is also available on request.
Microm H500 cryostat - for cutting frozen tissue sections
Cytocentrifuge - for preparation of cytospins
All other microscopy is carried out at the BIU (www.biu.soton.ac.uk)
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Costs
Individual groups may have to purchase specific primary antibodies or reagents specific for their particular study. All other general reagents, including commonly used antibodies, are supplied.
Fluorescence and confocal microscopes provided by BIU are charged at an hourly rate. (www.biu.soton.ac.uk)
Paraffin embedding and routine H&E staining of paraffin sections is carried out by HRU charged on a per block or per section basis. (http://www.hru.soton.ac.uk/)
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Contact:
Dr Sonya James (Mon-Thurs 9am-2pm)
Microscopy Support Officer
Tenovus Building (Office G9, Lab G7)
Ext: 4690
Email:sonya.james@soton.ac.uk
Recent Publications
James S, Tutt AL, Laversin SA, Tipton TR, Ashton-Key M, French RR, Hussain K, Vaughan AT, Dou L, Earley A, Dahal LN, Lu C, Dunscombe M, Chan HT, Penfold CA, Kim JH, Potter EA, Mockridge CI, Roghanian A, Oldham RJ, Cox KL, Lim SH, Teige I, Frendéus B, Glennie MJ, Beers SA, Cragg MS (2015) Development and Characterization of Monoclonal Antibodies Specific for Mouse and Human Fcγ Receptors. The Journal of Immunology 195(11):5503-16
Roghanian A, Teige I, Mårtensson L, Cox KL, Kovacek M, Ljungars A, Mattson J, Sundberg A, Vaughan AT, Shah V, Smyth NR, Sheth B, Chan HT, Li ZC, Williams EL, Manfredi G, Oldham RJ, Mockridge CI, James SA, Dahal LN, Hussain K, Nilsson B, Verbeek JS, Juliusson G, Hansson M, Jerkeman M, Johnson PW, Davies A, Beers SA, Glennie MJ, Frendéus B, Cragg MS (2015) Antagonistic human FcγRIIB (CD32B) antibodies have anti-tumor activity and overcome resistance to antibody therapy in vivo. Cancer Cell 27(4):473-88
Williams EL, Dunn SN, James S, Johnson PW, Cragg MS, Glennie MJ, Gray JC. (2013) Immunomodulatory monoclonal antibodies combined with peptide vaccination provide potent immunotherapy in an aggressive murine neuroblastoma model. Clin Cancer Res 19(13):3545-55
Buchan SL, Taraban VY, Slebioda TJ, James S, Cunningham AF, Al-Shamkhani A. (2012) Death receptor 3 is essential for generating optimal protective CD4⁺ T-cell immunity against Salmonella. Eur J Immunol 42(3):580-8
White AL, Tutt AL, James S, Wilkinson K, Castro FVV, Dixon SV, Hitchcok J, Khan M, Al-Shamkani A, Cunningham AF and Glennie MJ (2010) Ligation of CD11c during vaccination promotes germinal centre induction and robust humoral responses without adjuvant. Immunology 130(2):
Gray JC, French RR, James S, Al-Shamkhani A, Johnson PW, Glennie MJ (2008) Optimising anti-tumour CD8 T-cell responses using combinations of immunomodulatory antibodies. Eur. J. Immunol. 38: 2499-2511
Castro FVV, Tutt AL, White AL, Teeling JL, James S, French RR and Glennie MJ (2008) CD11c provides an effective immunotarget for the generation of both CD4 and CD8 T cell responses. Eur. J. Immunol. 38: 2263-2273
Beers SA, Chan CHT, James S, French RR, Attfield KE, Brennan CM, Ahuja A, Shlomchik MJ, Cragg MS, Glennie MJ (2008) Type II (tositumomab) anti-CD20 monoclonal antibody out performs type I (rituximab-like) reagents in B-cell depletion regardless of complement activation. Blood 112 (10): 4170-4177
Taraban VY, Martin S, Attfield KE, Glennie MJ, Elliott T, Elewaut D, Van Calenbergh S, Linclau B and Al-Shamkhani A (2008) Invariant NKT Cells Promote CD8-Cytotoxic T Cell Responses by Inducing CD70 Expression on Dendritic Cells. The Journal of Immunology 180: 4615-4620.