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The University of Southampton
Imaging and Microscopy Centre

Fluorescence, Brightfield and DIC microscopy

Zeiss Axioplan

Equipment: Zeiss Axioplan 2

Location: 85/3105 Highfield campus

Type: Upright, motorised fluorescence/brightfield microscope

Live imaging chamber: No

Objectives: 5x, 10x, 20x, 40x air, 40x oil, 63x oil, 100x oil.

DIC: Yes

Stage: Manual

Camera: CCD 2750 x 2200 pixels

Filtersets: DAPI, FITC, TRITC, CY5

Nikon Ti

Equipment:  Nikon Ti

Location: 85/3105 Highfield campus

Type: Inverted, motorised fluorescence/brightfield microscope

Live imaging chamber: Yes

Objectives: 4x, 10x, 20x, 40x air, 40x oil, 60x oil, 100x oil.

DIC: No

Stage: Motorised

Camera: CCD 1344 x 1024 pixels

Filtersets: DAPI, FITC, TRITC, CY5

Nikon E800

Equipment: Nikon E800

Location: 85/3105 Highfield campus

Type: Upright, manual fluorescence/brightfield microscope

Live imaging chamber: No

Objectives: 4x, 10x, 20x, 40x air, 40x oil, 60x oil, 100x oil.

DIC: Yes

Stage: Manual

Camera: sCMOS 1920 x 1080 pixels

Filtersets: DAPI, FITC, TRITC

Deltavision Elite

Equipment: Deltavision Elite Live Imaging system . Hardware autofocus and deconvolution

Location: 85/3105 Highfield campus

Type: Inverted, motorised fluorescence/brightfield microscope

Live imaging chamber: Yes

Objectives: 10x, 20x, 40x oil 60x oil.

DIC: Yes

Stage: Motorised

Camera: sCMOS 2200 x 2200 pixels

Filtersets: (LED) DAPI, CFP, FITC, YFP, TRITC, mCherry, CY5, high-speed FITC/TRITC

Leica DM4B

Equipment: Leica DM4B

Location: Level D Laboratory & Pathology Block, University Hospital Southampton

Type: Upright, motorised fluorescence/brightfield/Histology microscope

Live imaging chamber: No

Objectives: 10x, 20x, 40x, 63x

DIC: No

Stage: Manual

Camera: CCD Colour and monochrome

Filtersets: DAPI, FITC, TRITC

Leica DM5000B

Equipment: Leica DM5000B

Location: Level D Laboratory & Pathology Block, University Hospital Southampton

Type: Upright, motorised fluorescence/brightfield/Histology microscope

Live imaging chamber: No

Objectives: 10x, 20x, 40x, 63x

DIC: No

Stage: Manual

Camera: CCD Colour and monochrome

Filtersets: DAPI, FITC, TRITC

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