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Adenosine monophosphate

Adenosine monophosphate (AMP), also known as 5'-adenylic acid, is a nucleotide that is used as a monomer in DNA and RNA. It is an ester of phosphoric acid and the nucleoside adenosine. AMP consists of a phosphate group, the sugar ribose, and the nucleobase adenine.

Data Collection

Bruker AVIIIHD500 FT-NMR spectrometer

5 mm SMART probe

1D proton NMR spectrum. 1D proton spectra are typically acquired within minutes. The signals are integrated to show the relative ratio of proton signals in each chemical environment and the peak multiplicities can show the coupling network within the molecule.

1D phosphorus-31 NMR spectrum. 1D 31P NMR spectra can be acquired both with and without 1H decoupling (illustrated example is with decoupling).

HH COSY. The HH COSY shows the coupling network within the molecule.

HSQC. In the HSQC spectrum the one-bond direct HC couplings can be viewed as cross-peaks between the proton and carbon projections; in the illustrated spectrum, DEPT editing has been applied.

In the HMBC spectrum the two- and three- bond couplings between protons and carbons can be seen as cross-peaks that correctly align with both projections; the cross-peaks that align correctly only with the carbon projection and are split around the proton signal are one-bond direct HC couplings.

HP HMBC. In the HP HMBC spectrum the two- and three- bond couplings between 1H and 31P nuclei can be seen as cross-peaks.

In the HN HMBC spectrum the two- and three- bond couplings between 1H and 15N nuclei can be seen as cross-peaks. The f1 projection can be extracted and provide 15N chemical shifts for nitrogens proximal to protons much more rapidly than possible by direct 15N observation.

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