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The NMR Spectroscopy FacilityCapabilities



Sucrose ("table sugar") is a disaccharide derived from glucose and fructose. The interesting feature of the molecule from an NMR spectroscopy viewpoint is that the two monomer units are completely separate spin systems and this can be visualised in the TOCSY spectrum.

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Data Collection

AVIIIHD500 FT-NMR spectrometer

5 mm SMART probe

HH COSY. The HH COSY shows the coupling network within the molecule.
TOCSY. The HH TOCSY spectrum shows correlations that belong together in contiguous spin systems: in the sucrose example, this means that the protons in the respective glucose and fructose units can be assigned.
HSQC. In the HSQC spectrum the one-bond direct HC couplings can be viewed as cross-peaks between the proton and carbon projections.
NUS HSQC. In the HSQC spectrum acquired using non-uniform sampling data collection, the cross-peaks showing the one-bond direct HC couplings can be visualised with much greater resolution in the carbon dimension.
In the HMBC spectrum the two- and three- bond couplings between protons and carbons can be seen as cross-peaks that correctly align with both projections; the cross-peaks that align correctly only with the carbon projection and are split around the proton signal are one-bond direct HC couplings.
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