Chemistry

G John Langley

BSc PhD CChem CSci FRSC

Primary position:
Principal Research Fellow in Mass Spectrometry
Other positions:
Head of Characterisation and Analytics

Background

The University of Southampton

John Langley's Publications and Citation Metrics

John Langley has nearly 30 years’ experience in mass spectrometry and chromatography-mass spectrometry. He received a mass spectrometry PhD from the University of London where he started his working career.

John is a Chartered Chemist, a Fellow of the Royal Society of Chemistry (1999) and a Chartered Scientist (2004); he heads the Characterisation and Analytics section in Chemistry at Southampton, leads SCAS (Southampton Chemistry Analytical Solutions (http://www.southampton.ac.uk/scas/), is Chair of the Separation Science Group of the Royal Society of Chemistry (http://www.sep-sci.org.uk/) and is Secretary of the International Mass Spectrometry Foundation (http://www.imss.nl/). Formerly Chair, Vice-Chair, General Secretary and Meetings Secretary of the British Mass Spectrometry Society, a member of the Organisation Committee for the International Mass Spectrometry Conference for Edinburgh 2003 and Kyoto 2012, and BMSS Annual Conferences 1999, 2001, 2002, 2004, 2005 and 2007 and is the European Member of IUPAC Task Group for Standard Definitions of Terms Relating to Mass Spectrometry. He is a member of the EPSRC Peer Review College, a referee for Research Council and Charity research grant proposals, a member of the Editorial Board for Rapid Communications in Mass Spectrometry and a reviewer for all other leading mass spectrometry, chromatography and chemistry journals; has also been called as an expert witness; MS consultant; is a freelance Team Development trainer and involved in the organisation and implementation of the School of Chemistry key skills programmes.

To date, John has 80+ refereed publications; with an ‘h-index’ of 28 and over the years has been involved in the analysis of organometallics, oligonucleotides, bio-organic chemistry, natural products, proteins, supramolecular chemistry, target synthesis, combinatorial chemistry pharmaceutical and oilfield chemistries. External academic collaborations include early studies of fullerene MS with the Sussex group. This range of chemistries has led to publications in over 30 different journals.

During his time at Southampton, he has developed his own research group (http://www.southampton.ac.uk/~gjl/) and worked on MS problems that span the spectrum of chemical sciences, with other research groups both within and outside Southampton. External academic collaborators include AstraZeneca, BP, GSK, LGC, Pfizer, Waters and early studies of fullerenes with the Sussex group.

Dr G John Langley's photo

Web links

Publications

The University of Southampton's electronic library (e-prints)

Key Publications

Riley, Jo-Anne, Brown, Tom, Gale, Nittaya, Herniman, Julie and Langley, G. John (2012) Self reporting RNA probes as an alternative to cleavable small molecule mass tags. The Analyst, 137, (24), 5817-5822. (doi:10.1039/C2AN36086A ). (PMID:23096125).
Gale, Philip A, Felix, Vitor, Pérez Tomás, Ricardo, Soto Cerrato, Vanessa, Haynes, Cally, Moore, Stephen, González, Jorge, Light, Mark E., Sutton, Jennifer, Marques, Igor, Herniman, Julie and Langley, John (2013) Chloride, carboxylate and carbonate transport by ortho-phenylenediamine-based bisureas. Chemical Science, 4, (1), 103-117. (doi:10.1039/C2SC21112B).
Cazenave-Gassiot, Amaury, Boughtflower, Robert, Caldwell, Jeffrey, Hitzel, Laure, Holyoak, Claire, Lane, Stephen, Oakley, Paul, Pullen, Frank, Richardson, Stefan and Langley, G. John (2009) Effect of increasing concentration of ammonium acetate as an additive in supercritical fluid chromatography using CO2–methanol mobile phase. Journal of Chromatography A, 1216, (36), 6441-6450. (doi:10.1016/j.chroma.2009.07.022).
Hopley, Chris, Bristow, Tony, Lubben, Anneke, Simpson, Alec, Bull, Elaine, Klagkou, Katerina, Herniman, Julie and Langley, John (2008) Towards a universal product ion mass spectral library – reproducibility of product ion spectra across eleven different mass spectrometers. Rapid Communications in Mass Spectrometry, 22, (12), 1779-1786. (doi:10.1002/rcm.3545).

Article

Riley, Jo-Anne, Brown, Tom, Gale, Nittaya, Herniman, Julie and Langley, G. John (2014) Self-reporting hybridisation assay for miRNA analysis. The Analyst, 139, (5), 1088-1092. (doi:10.1039/C3AN01825C). (PMID:24404560).
Busschaert, Nathalie, Jaramillo-Garcia, Javier, Light, Mark E., Herniman, Julie, Langley, G. John and Gale, Philip A. (2014) An anion-binding fluorinated alcohol isophthalamide isostere. RSC Advances , 4, (11), 5389-5393. (doi:10.1039/C3RA45956J).
Al-Harrasi, Ahmed, Ali, Liaqat, Rehman, Najeeb Ur, Hussain, Hidayat, Hussain, Javid, Al-Rawahi, Ahmed, Langley, G. John, Wells, Neil J. and Abbas, Ghulam (2013) Nine triterpenes from Boswellia sacra Flückiger and their chemotaxonomic importance. Biochemical Systematics and Ecology, 51, 113-116. (doi:10.1016/j.bse.2013.08.026).
Murray, Kermit K., Boyd, Robert K., Eberlin, Marcos N., Langley, G. John, Li, Liang and Naito, Yasuhide (2013) Definitions of terms relating to mass spectrometry (IUPAC Recommendations 2013). Pure and Applied Chemistry (doi:10.1351/PAC-REC-06-04-06).
Galezowska, Angelika, Harrison, Mark W., Herniman, Julie M., Skylaris, Chris-Kriton and Langley, G. John (2013) A predictive science approach to aid understanding of electrospray ionisation tandem mass spectrometric fragmentation pathways of small molecules using density functional calculations. Rapid Communications in Mass Spectrometry, 27, (9), 964-970. (doi:10.1002/rcm.6536).
Gale, Philip A., Busschaert, Nathalie, Bradberry, Samuel J., Wenzel, Marco, Haynes, Cally, Hiscock, Jennifer R., Kirby, Isabelle, Karagiannidis, Louise E., Moore, Stephen J., Wells, Neil, Herniman, Julie, Langley, John, Horton, Peter, Light, Mark E., Marques, Igor, Costa, Paulo Jorge, Felix, Vitor and Frey, J.G. (2013) Towards predictable transmembrane transport: QSAR analysis of anion binding and anion transport. Chemical Science, 4, (8), 3036-3045. (doi:10.1039/C3SC51023A).
Moore, Stephen J., Haynes, Cally J. E., González, Jorge, Sutton, Jennifer L., Brooks, Simon J., Light, Mark E., Herniman, Julie, Langley, G. John, Soto-Cerrato, Vanessa, Pérez-Tomás, Ricardo, Marques, Igor, Costa, Paulo J., Félix, Vítor and Gale, Philip A. (2013) Chloride, carboxylate and carbonate transport by ortho-phenylenediamine-based bisureas. Chemical Science, 4, (1), 103-117. (doi:10.1039/c2sc21112b).
Tovey, Frank I., Capanoglu, Doga, Langley, G. John, Herniman, Julie M., Bor, Serhat, Ozutemiz, Omer, Hobsley, Michael, Bardhan, Karna Dev and Linclau, Bruno (2011) Dietary phytosterols protective against peptic ulceration. Gastroenterology Research, 4, (4), 149-156. (doi:10.4021/gr328w).
Barker, J., Langley, G. and Richards, P. (2010) Insights into deposit formation in high pressure diesel fuel injection equipment. SAE Technical Papers, 2010-01-2243 (doi:10.4271/2010-01-2243).
Pinto, R.M., Olariu, R.I., Lameiras, J., Martins, F.T., Dias, A.A., Langley, G.J., Rodrigues, P., Maycock, C.D., Santos, J.P., Duarte, M.F., Fernandez, M.T. and Costa, M.L. (2010) Study of selected benzyl azides by UV photoelectron spectroscopy and mass spectrometry. Journal of Molecular Structure, 980, (1-3), 163-171. (doi:10.1016/j.molstruc.2010.07.009).
Ball, Rachel J., Green, Philip S., Gale, Nittaya, Langley, G. John and Brown, Tom (2010) Peptide nucleic acid probes with charged photocleavable mass markers: towards PNA-based MALDI-TOF MS genetic analysis. Artificial DNA: PNA & XNA, 1, (1), 27-35. (doi:10.4161/adna.1.1.12199).
Abdel Ghani, Sherif B., Chapman, James M., Figadère, Bruno, Herniman, Julie M., Langley, G. John, Niemann, Scott and Brown, Richard C.D. (2009) Total synthesis and stereochemical assignment of cis-uvariamicin I and cis-reticulatacin. The Journal of Organic Chemistry, 74, (18), 6924-6928. (doi:10.1021/jo9012578).
Thite, Mohini A., Boughtflower, Robert, Caldwell, Jeff, Hitzel, Laure, Holyoak, Clare, Lane, Stephen J., Oakley, Paul, Pullen, Frank S., Richardson, Stefan and Langley, G. John (2008) Ionisation in the absence of high voltage using supercritical fluid chromatography: a possible route to increased signal. Rapid Communications in Mass Spectrometry, 22, (22), 3673-3682. (doi:10.1002/rcm.3784).
Cazenave-Gassiot, Amaury, Boughtflower, Robert, Caldwell, Jeffrey, Coxhead, Richard, Hitzel, Laure, Lane, Stephen, Oakley, Paul, Holyoak, Clare, Pullen, Frank and Langley, G. John (2008) Prediction of retention for sulfonamides in supercritical fluid chromatography. [in special issue: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques] Journal of Chromatography A, 1189, (1-2), 254-267. (doi:10.1016/j.chroma.2007.10.020).
Shepherd, Jon, Langley, G. John, Herniman, Julie M. and Kilburn, Jeremy D. (2007) Rapid sequencing of split-and-mix peptide receptor libraries: Identification of binding partners for Val-Val-Ile-Ala in aqueous solution. European Journal of Organic Chemistry, 2007, (8), 1345-1356. (doi:10.1002/ejoc.200601004).
Langley, G.J., Herniman, J.M. and Townell, M.S. (2007) 2B or not 2B, that is the question: further investigations into the use of pencil as a matrix for matrix-assisted laser desorption/ionisation. Rapid Communications in Mass Spectrometry, 21, (2), 180-190. (doi:10.1002/rcm.2827).
Hammond, N., Koumi, P., Langley, G.J., Lowe, A. and Brown, T. (2007) Rapid mass spectrometric identification of human genomic polymorphisms using multiplexed photocleavable mass-tagged probes and solid phase capture. Organic & Biomolecular Chemistry, 5, (12), 1878-1885. (doi:10.1039/b704587e).
Valappil, S.P., Peiris, D., Langley, G.J., Hemiman, J.M., Boccaccini, A.R., Bucke, C. and Roy, I. (2007) Polyhydroxyalkanoate (PHA) biosynthesis from structurally unrelated carbon sources by a newly characterized Bacillus spp. Journal of Biotechnology, 127, (3), 475-487. (doi:10.1016/j.jbiotec.2006.07.015).
Brondz, I., Ekeberg, D., Bell, D.S., Annino, A.R., Hustad, J.A., Svendsen, R., Vlachos, V., Oakley, P., Langley, G.J., Thite, M., Cazenave-Gassiot, A. and Mikhalitsyn, F. (2007) Nature of the main contaminant in the drug primaquine diphosphate: SFC and SFC-MS methods of analysis. Journal of Pharmaceutical and Biomedical Analysis, 43, (3), 937-944. (doi:10.1016/j.jpba.2006.09.017).
Black, Claudie, Poile, Chris, Langley, John and Herniman, Julie (2006) The use of pencil lead as a matrix and calibrant for matrix-assisted laser desorption/ionisation. Rapid Communications in Mass Spectrometry, 20, (7), 1053-1060. (doi:10.1002/rcm.2408).
Herniman, Julie M., Langley, G. John, Bristow, Tony W.T and O'Connor, Gavin (2005) The validation of exact mass measurements for small molecules using FT-ICRMS for improved confidence in the selection of elemental formulas. Journal of the American Society for Mass Spectrometry, 16, (7), 1100-1108. (doi: 10.1016/j.jasms.2005.02.027).
Darwish, Adam D., Birkett, Paul R., Langley, Graham and Taylor, Roger (2005) Fragmentation of the 60 fullerene cage during bromination. Chemical Physics Letters, 408, (4-6), 279-283. (doi:10.1016/j.cplett.2005.04.065).
Martins, Filipa, Duarte, M. Filomena, Fernandez, M. Tereza, Langley, G. John, Rodrigues, Paula, Barros, M. Teresa and Costa, M. Lourdes (2004) Electron ionization mass spectrometry in the characterization of azidonitriles. Rapid Communications in Mass Spectrometry, 18, (3), 363-366. (doi: 10.1002/rcm.1331).
Walker, Valerie, Mills, Graham A., Mellor, John M., Langley, G. John and Farrant, R. Duncan (2003) A novel pyrroline-5-carboxylic acid and acetoacetic acid adduct in hyperprolinaemia type II. Clinica Chimica Acta, 331, (1-2), 7-17. (doi:10.1016/S0009-8981(03)00077-9).
Klagkou, Katerina, Pullen, Frank, Harrison, Mark, Organ, Andy, Firth, Alistair and Langley, G. John (2003) Approaches towards the automated interpretation and prediction of electrospray tandem mass spectra of non-peptidic combinatorial compounds. Rapid Communications in Mass Spectrometry, 17, (11), 1163-1168. (doi: 10.1002/rcm.987).
Duarte, M. Filomena, Martins, Filipa, Fernandez, M. Tereza, Langley, G. John, Rodrigues, Paula, Barros, M. Teresa and Costa, M. Lourdes (2003) Mass spectrometric study of aliphatic α-carbonyl azides. Rapid Communications in Mass Spectrometry, 17, (9), 957-962. (doi: 10.1002/rcm.1005).
Klagkou, Katerina, Pullen, Frank, Harrison, Mark, Organ, Andy, Firth, Alistair and Langley, G. John (2003) Fragmentation pathways of sulphonamides under electrospray tandem mass spectrometric conditions. Rapid Communications in Mass Spectrometry, 17, (21), 2373-2379. (doi:10.1002/rcm.1201).
Bugg, Timothy D.H., Fleming, Sarah M., Robertson, Thomas A. and Langley, G. John (2002) 2-hydroxy-6-keto-nona-2,4-diene 1,9-dioic acid 5,6-hydrolase: evidence from O-18 isotope exchange for gem-diol intermediate. Methods in Enzymology, 354, 106-118. (doi:10.1016/S0076-6879(02)54008-8).
Farrant, R.D., Walker, V., Mills, G.A., Mellor, J.M. and Langley, G.J. (2001) Pyridoxal phosphate de-activation by pyrroline-5-carboxylic acid: increased risk of vitamin B6 deficiency and seizures in hyperprolinemia type II. Journal of Biological Chemistry, 276, (18), 15107-15116. (doi:10.1074/jbc.M010860200).
Swali, Vinay, Wells, Neil J., Langley, G. John and Bradley, Mark (1997) Solid-phase dendrimer synthesis and the generation of super-high-loading resin beads for combinatorial chemistry. The Journal of Organic Chemistry, 62, (15), 4902-4903. (doi:10.1021/jo9708654).
 

Research

Research Interests

Dr. G. John Langley Research Group

www.soton.ac.uk/~gjl

Research keywords

Accurate mass, biofuels, electrospray, FT-MS, FT-ICR MS, GC-MS, high throughput, HPLC-MS, MALDI, mass spectrometry, MS/MS, oligonucleotides, open access, petrochemicals, SFC-MS.

Research funding

12 - 16 Innospec, A Study of Fuels by Separation Science & High Resolution Mass Spectrometry. G. J. Langley, £40,000.

11 - 16 Wellcome Trust, Characterisation of lipid oxidation by high accuracy mass spectrometry. A. Postle, H. Clark, G. J. Langley, V. H. Perry, G. Clough, A. Pringle, R. Djukanovic, A. Smith, £398,152.

09 – 12 BBSRC/Pfizer CASE, Development and implementation of Mass-Tagged Oligonucelotides as Bioanalytical Probes. G. J. Langley, £73,368.

08 - 11 EPSRC/BP CASE, Development and Application of MS and Chromatography Techniques for the Analysis of Bio Derived Components in Fuels and Lubricants. G.J.Langley, £63,789.

08 - 11 AstraZeneca, Mass spectrometry applications in pharmaceutical chemistry. G. J. Langley, £54,000.

06 - 09 BBSRC/Pfizer CASE. Facilitating rapid metabolite identification by using MS to determine fragmentation patterns that may be characteristic of the site of metabolism. G. J. Langley, £47,558

 

Primary research group:  Characterisation and Analytics

Affiliate research group:  Molecular Assembly, Function and Structure

Research project

Facilities

Responsibilities

PhD supervision

A. Nasif (Y1), E. Elia (Y1), K. Patel (Y2) W. Ratsameepakai (Y3)

Teaching Responsibilities

John is involved in organisation and implementation of the Chemistry's key skills programmes.

 

Contact

Dr G John Langley
Chemistry
University of Southampton
Highfield
Southampton
SO17 1BJ

Room Number: 30/1067

Telephone: (023) 8059 2182
Email: G.J.Langley@soton.ac.uk