Medicine

Jonathan Strefford

BSc., PCCC, PhD

Primary position:
Reader in Cancer Molecular Genetics

Background

The University of Southampton

Dr Jonathan Strefford is a Reader (Associate Professor) in Cancer Molecular Genetics in the Faculty of Medicine at the University of Southampton. After two years NHS training in clinical cytogenetics, Jon started his postgraduate education with a Ph.D. in Cancer Genetics and Genetic Toxicology at the University of Wales, Swansea. In 2000, he started his postdoctoral careers, working with Professor Bryan Young at the Cancer Research UK Medical Oncology Laboratory at St Bartholomew’s Hospital, London. In 2002, he received a Lectureship from Queen Mary and Westfield College, University of London, prior to a move to the University of Southampton. In 2008, after four years working with Professor Christine Harrison, Jon was awarded a Personal Fellowship from Leukaemia and Lymphoma Research and a Senior Lectureship within the Faculty of Medicine. Jon currently has a research group working on the genetic analysis of chronic lymphocytic leukaemia (CLL) and splenic marginal zone lymphoma (SMZL).

To facilitate these research objectives, Dr Strefford has attracted more than £4.5 million in grant funding from national and international research agencies and charities. He has spoken at international meetings such as the American Society of Hematology and the American Association for Cancer Research. Dr Strefford acts as an editorial board member for a number of international scientific journals, and on a number of national and international scientific committees.



Dr Strefford has extensive experience as a consultant for commercial companies, facilitating the development of FISH and microarray products. He also has strong links with the pharmaceutical industry and acts as a consultant and contract research provider for a series of large international clinical trials.

Dr Strefford’s Linkedin and ResearchGate profiles.

Cancer Genomics Group Facebook Page

Qualifications

BSc., Biological Sciences, University of Plymouth (1994)
PCCC, Association of Clinical Cytogenetics (1996)
Ph.D. Genetic Toxicology, University of Wales Swansea (2000)

Appointments held

Reader and Group Leader, Cancer Sciences Academic Unit, Faculty of Medicine, University of Southampton (2012- to date)
Honorary Clinical Scientist, Wessex Regional Genetics Laboratory, Salisbury District Hospital (2006- to date)
Honorary Senior Biomedical Scientist, Department of Pathology, Royal Bournemouth Hospital (2012- to date)

Previous appointments held

Senior Lecturer and Group Leader, Cancer Sciences Academic Unit, Faculty of Medicine, University of Southampton (2008-2012)
Senior Research Fellow, Cancer Sciences Academic Unit, Faculty of Medicine, University of Southampton (2004-2008)


Lecturer in Molecular Oncology, CRUK Department of Medical Oncology, Institute of Cancer, Queen Mary and Westfield College (2002-2004)


Research Fellow, CRUK Medical Oncology Unit, St Bartholomew’s Hospital, London (1999-2002)



Dr Jonathan Strefford's photo

Publications

The University of Southampton's electronic library (e-prints)

Article

Rose-Zerilli, Matthew J., Forster, Jade, Parker, Helen, parker, Anton, Gardiner, Anne, Collins, A., Stankovic, Tanja, Oscier, D.G. and Strefford, Jonathan C. (2014) ATM mutation rather than BIRC3 deletion and/or mutation predicts reduced survival in 11q-deleted chronic lymphocytic leukemia: data from the UK LRF CLL4 trial. Haematologica, 99, (4), 736-742. (doi:10.3324/haematol.2013.098574). (PMID:24584352).
Parry, Marina, Rose-Zerilli, Matthew J. J., Gibson, Jane, Ennis, Sarah, Walewska, Renata, Forster, Jade, Parker, Helen, Davis, Zadie, Gardiner, Anne, Collins, Andrew, Oscier, David G. and Strefford, Jonathan C. (2013) Whole exome sequencing identifies novel recurrently mutated genes in patients with splenic marginal zone lymphoma. PLoS ONE, 8, (12), e83244. (doi:10.1371/journal.pone.0083244). (PMID:24349473).
Zhang, L., Pickard, K., Bullock, M.D., Jenei, V., Strefford, J., Mitter, R., Kelly, G., Primrose, J.N., Thomas, G., Packham, G.K. and Mirnezami, A.H. (2013) miR-153 supports colorectal cancer progression via pleiotropic effects that enahance invasion and chemotherapeutic resistance. Cancer Research, 73, 6435-6447. (doi:10.1158/0008-5472.CAN-12-3308). (PMID:23950211).
Chigrinova, Ekaterina, Rinaldi, Andrea, Kwee, Ivo, Rossi, Davide, Rancoita, Paola M.V., Strefford, Jonathan C., Oscier, David, Stamatopoulos, Kostas, Papadaki, Theodora, Berger, Francoise, Young, Ken H., Murray, Fiona, Rosenquist, Richard, Greiner, Timothy C., Chan, Wing C., Orlandi, Ester M., Lucioni, Marco, Marasca, Roberto, Inghirami, Giorgio, Ladetto, Marco, Forconi, Francesco, Cogliatti, Sergio, Votavova, Hana, Swerdlow, Steven H., Stilgenbauer, Stephan, Piris, Miguel A., Matolcsy, Andras, Spagnolo, Dominic, Nikitin, Eugene, Zamò, Alberto, Gattei, Valter, Bhagat, Govind, Ott, German, Zucca, Emanuele, Gaidano, Gianluca and Bertoni, Francesco (2013) Two main genetic pathways lead to the transformation of chronic lymphocytic leukemia to Richter Syndrome. Blood (doi:10.1182/blood-2013-03-489518). (PMID:24004666).
Strefford, J.C., Sutton, L-A., Baliakas, P., Agathangelidis, A., Malčíková, J., Plevova, K., Scarfó, L., Davis, Z., Stalika, E., Cortese, D., Cahill, N., Pedersen, L.B., di Celle, P.F., Tzenou, T., Geisler, C., Panagiotidis, P., Langerak, A.W., Chiorazzi, N., Pospisilova, S., Oscier, D.G., Davi, F., Belessi, C., Mansouri, L., Ghia, P., Stamatopoulos, K. and Rosenquist, R. (2013) Distinct patterns of novel gene mutations in poor-prognostic stereotyped subsets of chronic lymphocytic leukemia: the case of SF3B1 and subset #2. Leukemia (doi:10.1038/leu.2013.98 ). (PMID:23558524).
Oscier, D.G., Rose-Zerilli, M.J.J., Winkelmann, N., de Castro, D.G., Gomez, B., Forster, J., Parker, Helen, Parker, A., Gardiner, A., Collins, Andrew, Else, M., Cross, N.C.P., Catovsky, D. and Strefford, J.C. (2013) The clinical significance of NOTCH1 and SF3B1 mutations in the UK LRF CLL4 trial. Blood, 121, (3), 468-475. (doi:10.1182/blood-2012-05-429282). (PMID:23086750).
Gardiner, Anne, Parker, Helen, Glide, Sharron, Mould, Sarah, Robinson, Hazel M., Tracy, Ian, Stankovic, Tanja, Oscier, David G. and Strefford, Jon C. (2012) A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia. Leukemia Research, 36, (3), 307-310. (doi:10.1016/j.leukres.2011.08.002). (PMID:21955805).
Sinclair, Paul B., Parker, Helen, An, Qian, Rand, Vikki, Ensor, Hannah, Harrison, Christine J. and Strefford, Jonathan C. (2011) Analysis of a breakpoint cluster reveals insight into the mechanism of intrachromosomal amplification in a lymphoid malignancy. Human Molecular Genetics, 20, (13), 2591-2602. (doi:10.1093/hmg/ddr159). (PMID:21487021).
Rand, Vikki, Parker, Helen, Russell, Lisa J, Schwab, Claire, Ensor, Hannah, Irving, Julie, Jones, Lisa, Masic, Dino, Minto, Lynne, Morrison, Heather, Ryan, Sarra, Robinson, Hazel, Sinclair, Paul, Moorman, Anthony V, Strefford, Jonathan C. and Harrison, Christine J. (2011) Genomic characterization implicates iAMP21 as a likely primary genetic event in childhood B-cell precursor acute lymphoblastic leukemia. Blood, 117, (25), 6848-55. (doi:10.1182/blood-2011-01-329961). (PMID:21527530).
Parker, H., Rose-Zerilli, M.J.J., Parker, A., Chaplin, T., Wade, R., Gardiner, A., Griffiths, M., Collins, A., Young, B.D., Oscier, D.G. and Strefford, J.C. (2011) 13q deletion anatomy and disease progression in patients with chronic lymphocytic leukemia. Leukemia, 25, (3), 489-497. (doi:10.1038/leu.2010.288). (PMID:21151023).
Allan, J.M., Bailey, J.R., Pettitt, A.R., Harris, R.J., Pepper, C., Fegan, C., Strefford, J.C., Oscier, D.G., Pratt, G. and Allsup, D.J. (2010) Letter. Variant IRF4|[sol]|MUM1 associates with CD38 status and treatment-free survival in chronic lymphocytic leukaemia. Leukemia (doi:10.1038/leu.2009.298).
Allan, J.M., Bailey, J.R., Pettitt, A.R., Harris, R.J., Pepper, C, Fegan, C, Houlston, R.S., Sunter, N.J., Hall, A.G., Butt, J.C., Mainou-Fowler, T, Jackson, G, Summerfield, G, Evans, P, Strefford, J.C., Oscier, D.G., Pratt, G and Allsup, D.J. (2010) A common low penetrance risk allele for chronic lymphocytic leukaemia in the 3’ UTR of IRF4/MUM1 predicts prognosis independent of 1gVH status. Leukemia (doi:10.1038/leu.2009.298).
An, Q., Burke, G.A.A., Harrison, C.J., Kempski, H., Konn, Z.J., Strefford, J.C. and Martineau, M. (2009) Haploinsufficiency of the MLL and TOB2 genes in lymphoid malignancy. Leukemia (doi:10.1038/leu.2009.238).
Russell, Lisa J., Capasso, Melania, Vater, Inga, Bernard, Olivier A., Chandrasekaran, Thiruppavaii, Griffith, Mike, Irving, Julie, Kearney, Lyndal, Moorman, Anthony V., Rand, Vikki, Strefford, Jonathan C., Dyer, Martin J. S., Siebert, Reiner, Harrison, Christine J., Takashi, Akasaka, Calasanz, Maria Jose, Chapiro, Elise, Gesk, Stephan, Guttery, David S., Haferlach, Claudia, Harder, Lana, Heidenreich, Olaf, Nguyen-Khac, Florence, Machado, Lee, Minto, Lynne, Majid, Aneela, Morrison, Heather, Schwab, Claire and Tönnies, Holger (2009) Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. Blood, 114, (13), 2688-2698. (doi:10.1182/blood-2009-03-208397).
An, Qian, Wright, Sarah L., Moorman, Anthony V., Parker, Helen, Griffiths, Mike, Ross, Fiona M., Davies, Teresa, Harrison, Christine J. and Strefford, Jon C. (2009) Heterogeneous breakpoints in patients with acute lymphoblastic leukaemia and dic(9;20)(p11~13;q11) show recurrent gene involvement at 20q11.21. Haematologica, 94, (8), 1164-1169. (doi:10.3324/haematol.2008.002808).
Strefford, Jonathan C., An, Qian and Harrison, Christine J. (2009) Modeling the molecular consequences of unbalanced translocation in cancer; lessons from leukemia. Cell Cycle, 8, (14), 2175-2184.
Paulsson, Kajsa, An, Qian, Moorman, Anthony V., Parker, Helen, Molloy, Gael, Davies, Teresa, Griffiths, Mike, Ross, Fiona M., Irving, Julie, Harrison, Christine J., Young, Bryan D. and Strefford, Jon C. (2009) Methylation of tumour suppressor gene promoters in the presence and absence of transcriptional silencing in high hyperdiploid acute lymphoblastic leukaemia. British Journal of Haematology, 144, (6), 838-847. (doi:10.1111/j.1365-2141.2008.07523.x). (Submitted).
Sulong, Sarina, Moorman, Anthony V., Irving, Julie A.E., Strefford, Jonathan C., Konn, Zoe J., Case, Marian C., Minto, Lynne, Barber, Kerry E., Parker, Helen, Wright, Sarah L., Stewart, Adam R.M., Bailey, Simon, Bown, Nick P., Hall, Andrew and Harrison, Christine J. (2009) A comprehensive analysis of the CDKN2A gene in childhood acute lymphoblastic leukaemia reveals genomic deletion, copy number neutral loss of heterozygosity and association with specific cytogenetic subgroups. Blood, 113, (1), 100-107. (doi:10.1182/blood-2008-07-166801).
An, Qian, Wright, Sarah L., Moorman, Anthony V., Parker, Helen, Griffiths, Mike, Ross, Fiona M., Davies, Teresa, Harrison, Christine J. and Strefford, Jon C. (2009) Heterogeneous breakpoints in patients with acute lymphoblastic leukaemia and dic(9;20)(p11~13;q11) show recurrent involvement of genes at 20q11.21. Haematologica The Hematology Journal, 94, (8), 1164-1169. (doi:10.3324/haematol.2008.002808).
An, Qian, Wright, Sarah L., Konn, Zoë J., Matheson, Elizabeth, Minto, Lynne, Moorman, Anthony V., Parker, Helen, Griffiths, Mike, Ross, Fiona M., Davies, Teresa, Hall, Andy G., Harrison, Christine J., Irving, Julie A. and Strefford, Jon C. (2008) Variable breakpoints target PAX5 in patients with dicentric chromosomes: a model for the basis of unbalanced translocations in cancer. Proceedings of the National Academy of Sciences of the Unites States of America, 105, (44), 17050-17054. (doi:10.1073/pnas.0803494105). (PMID:18957548).
Moorman, A.V., Sulong, S., Irving, J.A.E., Strefford, J.C., Case, M.C., Minto, L., Harrison, C.J. and Hall, A.G. (2008) Inactivation of CDKN2A in childhood acute lymphoblastic leukaemia (ALL) occurs principally by deletion and is strongly correlated with cytogenetic subgroups. British Journal of Haematology, 141, (s1), p.106. (doi:10.1111/j.1365-2141.2008.07061.x).
Strefford, J.C., An, Q., Wright, S.L., Konn, Z.J., Matheson, E., Minto, L., Parker, H., Moorman, A.V., Hall, A., Irving, J. and Harrison, C.J. (2008) Heterogeneous breakpoints target PAX5 in acute lymphoblastic leukaemia patients with dicentric chromosomes. British Journal of Haematology, 141, (s1), p.24. (doi:10.1111/j.1365-2141.2008.07061.x).
Parker, Helen, Cheung, Kan L., Robinson, Hazel M., Harrison, Christine J. and Strefford, Jon C. (2008) Cytogenetic and genomic characterization of cell line ARH77. Cancer Genetics and Cytogenetics, 181, (1), 40-45. (doi:10.1016/j.cancergencyto.2007.10.013).
Russell, Lisa J., Akasaka, Takashi, Majid, Aneela, Sugimoto, Kei-ji, Karran, E. Loraine, Nagel, Inga, Harder, Lana, Claviez, Alexander, Gesk, Stefan, Moorman, Anthony V., Ross, Fiona, Mazzullo, Helen, Strefford, Jonathan C., Siebert, Reiner, Dyer, Martin J.S. and Harrison, Christine J. (2008) t(6;14)(p22;q32): a new recurrent IGH@ translocation involving ID4 in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Blood, 111, (1), 387-391. (doi:10.1182/blood-2007-07-092015). (PMID:17940204).
Russell, Lisa J., Akasaka, Takashi, Majid, Aneela, Sugimoto, Kei-ji, Karran, E. Loraine, Nagel, Inga, Harder, Lana, Claviez, Alexander, Gesk, Stefan, Moorman, Anthony V., Ross, Fiona, Mazzullo, Helen, Strefford, Jonathan C., Siebert, Reiner, Dyer, Martin J.S. and Harrison, Christine J. (2008) t(6;14)(p22;q32): a new recurrent IGH@ translocation involving ID4 in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Blood, 111, (1), 387-391. (doi:10.1182/blood-2007-07-092015).
Jalali, G.R., An, Q., Konn, Z.J., Worley, H., Wright, S.L., Harrison, C.J., Strefford, J.C. and Martineau, M. (2008) Disruption of ETV6 in intron 2 results in upregulatory and insertional events in childhood acute lymphoblastic leukaemia. Leukemia, 22, (1), 114-123. (doi:10.1038/sj.leu.2404994). (PMID:17972957).
Russell, L.J., Akasaka, T., Majid, A., Sugimoto, K.J., Karran, E.L., Nagel, I., Harder, L., Claviez, A., Gesk, S., Moorman, A.V., Ross, F., Mazzullo, H., Strefford, J.C., Siebert, R., Dyer, M.J.S. and Harrison, C.J. (2008) t(6;14)(p22;q32): a new recurrent IGH@ translocation involving ID4 in B-cell precursor acute lymphoblastic leukemia (BCP-ALL)2. Blood, 111, (1), 387-391. (doi:10.1182/blood-2007-07-092015).
Parker, Helen, An, Qian, Barber, Kerry, Case, Marian, Davies, Teresa, Konn, Zoë, Stewart, Adam, Wright, Sarah, Griffiths, Mike, Ross, Fiona M., Moorman, Anthony V., Hall, Andy G., Irving, Julie A., Harrison, Christine J. and Strefford, Jon C. (2008) The complex genomic profile of ETV6-RUNX1 positive acute lymphoblastic leukemia highlights a recurrent deletion of TBLIXR1. Genes, Chromosomes and Cancer, 47, (12), 1118-1125. (doi:10.1002/gcc.20613).
Harrison, C.J., Barber, K., Broadfield, Z., Stewart, A., Wright, S., Martineau, M., Strefford, J C. and Moorman, A.V. (2007) Cytogenetic classification of T lineage acute lymphoblastic leukaemia. British Journal of Haematology, 137, (s1), 18-19. (doi:10.1111/j.1365-2141.2007.06557.x).
Strefford, J.C., Worley, H., Barber, K., Stewart, A.R.M., An, Q., Robinson, H.M., Moorman, A.V. and Harrison, C.J. (2007) Genome complexity in patients with B-lineage acute lymphoblastic leukaemia revealed by array-based comparative genomic hybridization. British Journal of Haematology, 137, (s1), p.57. (doi:10.1111/j.1365-2141.2007.06557.x).
Barber, Kerry E., Harrison, Christine J., Broadfield, Zoe J., Stewart, Adam R.M., Wright, Sarah L., Martineau, Mary, Strefford, Jon C. and Moorman, Anthony V. (2007) Molecular cytogenetic characterization of TCF3 (E2A)/19p13.3 rearrangements in B-cell precursor acute lymphoblastic leukemia. Genes, Chromosomes and Cancer, 46, (5), 478-486. (doi:10.1002/gcc.20431).
Robinson, Hazel M., Harrison, Christine J., Moorman, Anthony V., Chudoba, Ilse and Strefford, Jonathan C. (2007) Intrachromosomal amplification of chromosome 21 (iAMP21) may arise from a breakage-fusion-bridge cycle. Genes, Chromosomes and Cancer, 46, (4), 318-326. (doi:10.1002/gcc.20412).
Akasaka, Takashi, Balasas, Theodore, Russell, Lisa J., Sugimoto, Kei-ji, Majid, Aneela, Walewska, Renata, Karran, E. Loraine, Brown, David G., Cain, Kelvin, Harder, Lana, Gesk, Stefan, Martin-Subero, Jose Ignacio, Atherton, Mark G., Brüggemann, Monika, Calasanz, María José, Davies, Teresa, Haas, Oskar A., Hagemeijer, Anne, Kempski, Helena, Lessard, Michel, Lillington, Debra M., Moore, Sarah, Nguyen-Khac, Florence, Radford-Weiss, Isabelle, Schoch, Claudia, Struski, Stéphanie, Talley, Polly, Welham, Melanie J., Worley, Helen, Strefford, Jon C., Harrison, Christine J., Siebert, Reiner and Dyer, Martin J.S. (2007) Five members of the CEBP transcription factor family are targeted by recurrent IGH-translocations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Blood, 109, (8), 3451-3461. (doi:10.1182/blood-2006-08-041012).
Moorman, Anthony V., Richards, Susan M., Robinson, Hazel M., Strefford, Jon C., Gibson, Brenda E.S., Kinsey, Sally E., Eden, Tim O.B., Vora, Ajay J., Mitchell, Christopher D. and Harrison, Christine J. On behalf of the UK Medical Research Council (MRC) / National Cancer Research Institute (NCRI) Childhood Leukaemia Working Party (CLWP) (2007) Prognosis of children with acute lymphoblastic leukaemia (ALL) and intrachromosomal amplification of chromosome 21 (iAMP21). Blood, 109, (6), 2327-2330. (doi:10.1182/blood-2006-08-040436).
An, Q., Wright, S.L., Konn, Z.J., Moorman, A.V., Harrison, C.J. and Strefford, J.C. (2007) Disruption of PAX5 in patients with acute lymphoblastic leukaemia and dicentric chromosomes. Journal of Medical Genetics, 44, pp.S32.
Strefford, J.C., Worley, H., Barber, K., Wright, S., Stewart, A.R.M., Robinson, H.M., Bettney, G., van Delft, F.W., Atherton, M.G., Davies, T., Griffiths, M., Hing, S., Ross, F.M., Talley, P., Saha, V., Moorman, A.V. and Harrison, C.J. (2007) Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization. Oncogene, 26, (29), 4306-4318. (doi:10.1038/sj.onc.1210190).
Akasaka, T., Balasas, T., Russell, L.J., Sugimoto, K., Majid, A., Walewska, R., Karran, E.L., Brown, D.G., Cain, K., Harder, L., Gesk, S., Martin-Subero, J.I., Atherton, M.G., Bruggemann, M., Calasanz, M.J., Davies, T., Haas, O.A., Hagemeijer, A., Kempski, H., Lessard, M., Lillington, D.M., Moore, S., Nguyen-Khac, F., Radford-Weiss, I., Schoch, C., Struski, S., Talley, P., Welham, M.J., Worley, H., Strefford, J. C., Harrison, C. J., Siebert, R. and Dyer, M.J.S. (2007) Five members of the CEBP transcription factor family are targeted by recurrent IGH translocations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Blood, 109, (8), 3451-3461. (doi:10.1182/blood-2006-08-041012).
Moorman, A.V., Richards, S.M., Robinson, H.M., Strefford, J.C., Gibson, B.E.S., Kinsey, S.E., Eden, T.O.B., Voras, A.J., Mitchell, C.D. and Harrison, C.J. (2007) Prognosis of children with acute lymphoblastic leukemia (ALL) and intrachromosomal amplification of chromosome 21 (iAMP21)30. Blood, 109, (6), 2327-2330. (doi:10.1182/blood-2006-08-040436).
Strefford, J., Worley, H., Barber, K J., Wright, S.L., Moorman, A.V., Case, M., Irving, J., Hall, A. and Harrison, C.J. (2007) Genomic profiling reveals multiple genetic targets in ETV6-RUNX1 positive acute lymphoblastic leukaemia (ALL). Journal of Medical Genetics, 44, pp.S33.
Strefford, J., Worley, H., Van Delft, F., Robinson, H., Barber, K., Saha, V., Moorman, A. and Harrison, C. (2007) Genomic and expression profiling defines the molecular features of intrachromosomal amplification of chromosome 21 in acute lymphoblastic leukemia. Cellular Oncology, 29, (2), 160-161.
Harrison, Christine J., Barber, Kerry, Broadfield, Zoë, Stewart, Adam, Wright, Sarah, Martineau, Mary, Strefford, Jon C. and Moorman, Anthony V. (2006) Cytogenetic classification of T lineage acute lymphoblastic leukaemia: multiple partners of BCL11B and other novel rearrangements. Blood, 108, (11), p.584A.
Strefford, Jon C., Worley, Helen, Barber, Kerry, Moorman, Anthony V. and Harrison, Christine J. (2006) Genome complexity in patients with acute lymphoblastic leukaemia revealed by array-based comparative genomic hybridization. Blood, 108, (11), 584A-584A.
Strefford, J.C., Lane, T.M., Hill, A., LeRoux, L., Foot, N.J., Shipley, J., Oliver, R.T., Lu, Y.J. and Young, B.D. (2006) Molecular characterisation of the t(1;15)(p22;q22) translocation in the prostate cancer cell line LNCaP. Cytogenetic and Genome Research, 112, (1-2), 45-52. (doi:10.1159/000087512).
Hanks, Sandra, Coleman, Kim, Summersgill, Brenda, Messahel, Boo, Williamson, Dan, Pritchard-Jones, Kathryn, Strefford, Jon, Swansbury, John, Plaja, Alberto, Shipley, Janet and Rahman, Nazneen (2006) Comparative genomic hybridization and BUB1B mutation analyses in childhood cancers associated with mosaic variegated aneuploidy syndrome. Cancer Letters, 239, (2), 234-238. (doi:10.1016/j.canlet.2005.08.006).
Strefford, Jon C., van Delft, Frederick W., Robinson, Hazel M., Worley, Helen, Yiannikouris, Olga, Selzer, Rebecca, Richmond, Todd, Hann, Ian, Bellotti, Tony, Raghavan, Manoj, Young, Bryan D., Saha, Vaskar and Harrison, Christine J. (2006) Complex genomic alterations and gene expression in patients with acute lymphoblastic leukemia and intrachromosomal amplification of chromosome 21. Proceedings of the National Academy of Sciences of the United States of America, 103, (21), 8167-8172. (doi:10.1073/pnas.0602360103).
Chapiro, Elise, Russell, Lisa, Radford-Weiss, Isabelle, Bastard, Christian, Lessard, Michel, Struski, Stephanie, Cave, Helene, Fert-Ferrer, Sandra, Barin, Carole, Maarek, Odile, Della-Valle, Veronique, Strefford, Jonathan C., Berger, Roland, Harrison, Christine J., Bernard, Olivier A. and Nguyen-Khac, Florence the Groupe Francophone de Cytogénétique Hématologique (2006) Overexpression of CEBPA resulting from the translocation t(14;19)(q32;q13) of human precursor B acute lymphoblastic leukemia. Blood, 108, (10), 3560-3563. (doi:10.1182/blood-2006-03-010835).
Dyer, Martin J.S., Akasaka, Takashi, Balasas, Theodore, Russell, Lisa, Sugimoto, Kei-Ji, Majid, Aneela, Brown, David G., Cain, Kelvin, Strefford, Jon C., Harrison, Christine J. and Siebert, Reiner (2005) Involvement of the CEBP gene family in four IGH@ chromosomal translocations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Blood, 106, (11), p.797A.
Robinson, Hazel M., Harrison, Christine J., Selzer, Rebecca, van Delft, Frederik and Strefford, Jon C. (2005) A breakage-fusion-bridge cycle generated by telomeric loss may underlie the dup(21q) of acute lymphoblastic leukemia. Blood, 106, (11), p.797A.
Robinson, H.M., Martineau, M., Harris, R.L., Barber, K.E., Jalali, G.R., Moorman, A.V., Strefford, J.C., Broadfield, Z.J., Cheung, K.L. and Harrison, C.J. (2005) Derivative chromosome 9 deletions are a significant feature of childhood Philadelphia chromosome positive acute lymphoblastic leukaemia. Leukemia, 19, (4), 564-571. (doi:10.1038/sj.leu.2403629).
Harrison, Christine J., Moorman, Anthony V., Barber, Kerry E., Broadfield, Zoë J., Cheung, Kan L., Harris, Rachel, Jalali, G. Reza, Robinson, Hazel M., Strefford, Jonathan C., Stewart, Adam, Wright, Sarah, Griffiths, Mike, Ross, Fiona M., Harewood, Louise and Martineau, Mary (2005) Interphase molecular cytogenetic screening for chromosomal abnormalities of prognostic significance in childhood acute lymphoblastic leukaemia: a UK Cancer Cytogenetics Group Study. British Journal of Haematology, 129, (4), 520-530. (doi:10.1111/j.1365-2141.2005.05497.x).
Strefford, Jon C., Stasevich, Irina, Lane, Tim M., Lu, Yong-Jie, Oliver, Tim and Young, Bryan D. (2005) A combination of molecular cytogenetic analyses reveals complex genetic alterations in conventional renal cell carcinoma. Cancer Genetics and Cytogenetics, 159, (1), 1-9. (doi:10.1016/j.cancergencyto.2004.09.020).
Wilson, Charlotte, Yang, Jinshu, Strefford, Jon C., Summersgill, Brenda, Young, Bryan D., Shipley, Janet, Oliver, Tim and Lu, Yong-Jie (2005) Overexpression of genes on 16q associated with cisplatin resistance of testicular germ cell tumor cell lines. Genes, Chromosomes and Cancer, 43, (2), 211-216. (doi:10.1002/gcc.20173).
Barber, K.E., Martineau, M., Harewood, L., Stewart, M., Cameron, E., Strefford, J.C., Rutherford, S., Allen, T.D., Broadfield, Z.J., Cheung, K.L., Harris, R.L., Jalali, G.R., Moorman, A.V., Robinson, H.M. and Harrison, C.J. (2004) Correspondence. Amplification of the ABL gene in T-cell acute lymphoblastic leukemia. Leukemia, 18, (6), 1153-1156. (doi:10.1038/sj.leu.2403357).
Strefford, J., Griffiths, M.J., Ross, F.M. and Harrision, C.J. (2004) Comparative genomic hybridisation onto array slides reveals specific DNA copy number changes in acute lymphoblastic leukaemia (ALL). Journal of Medical Genetics, 41, pp.S22.
Strefford, Jon C., Lillington, Debra M., Steggall, Martin, Lane, Tim M., Nouri, A.M.E, Young, Bryan D. and Oliver, R.T.D. (2002) Novel chromosome findings in bladder cancer cell lines detected with multiplex fluorescence in situ hybridization. Cancer Genetics and Cytogenetics, 135, (2), 139-146. (doi:10.1016/S0165-4608(01)00648-3).
Strefford, J.C., Foot, N.J., Chaplin, T., Neat, M.J., Oliver, R.T.D., Young, B.D. and Jones, L.K. (2001) The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH. Cytogenetics and Cell Genetics, 94, (1-2), 9-14. (doi:10.1159/000048774).

Book Section

Strefford, Jon C. and Parker, Helen (2009) Array-based comparative genomic hybridization as a tool for analyzing the leukaemia genome. In, Wai, Chi and So, Eric (eds.) Leukemia - Methods and Protocols. New York, USA, Humana Press, 151-178. (Spinger Protocols - Methods in Molecular Biology 538). (doi:10.1007/978-1-59745-418-6_8).
Strefford, Jon (2003) Solving problems in multiplex FISH. In, Swansbury, John (ed.) Cancer Cytogenics: Methods and Protocols. Totowa, USA, Humana, 235-244. (Methods in Molecular Biology, 220). (doi:10.1226/1588290808).
 

Research

Research Interests

Dr Strefford’s research has focused on the identification and characterization of genomic defects in cancer cells.

Genomic abnormalities are the hallmark of human cancer and can aid in appropriate patient management. The presence of these genomic alterations can have utility as Biomarkers by providing guidance on the most appropriate treatment regime and identify those patients likely to develop progressive disease or those that might exhibit limited response to treatment.

The research projects coordinated by Dr Strefford include:

  1. Biomarker Discovery: Using whole genome analysis for copy number changes and sequence mutations to screen clinically-informative leukaemia and lymphoma cohorts.
  2. Biomarker Validation: Using target molecular and NGS technologies to determine the clinical impact of genomic lesions on the patient survival and response to treatment, particularly in the clinical trials context.
  3. Functional Analysis: Using in vitro and in vivo model systems we assess the role of novel genetic lesions in the pathophysiology of B-cell neoplams.

Funding

  1. Cragg M, Beers SA, Johnson P, Davies A, Strefford JC (2013) Optimizing antibody treatment for patients with lymphoma. Leukaemia and Lymphoma Research Specialist Programme Grant (£1.3Million)
  2. Strefford JC, Steele A, Cragg M, Vorechovsky I (2012) Importance of SF3B1 mutations in the diagnosis, prognosis and biology of chronic lymphocytic leukaemia. Leukaemia and Lymphoma Research Project Grant (£230K)
  3. Strefford JC (2012) The importance of novel genetic lesions in patients with chronic lymphocytic leukaemia. Bournemouth Leukaemia Fund PhD Studentship (£100K)
  4. Strefford JC, Davies A (2012) FC Receptor analysis in the ROCHE GOYA Trial. ROCHE Pharmaceuticals Project Grant (£130K)
  5. Strefford JC, Collins A, Oscier DG (2012) The identification and characterization of novel genetic lesions associated with clinical outcome in patients with Splenic Marginal Zone Lymphoma. Leukaemia and Lymphoma Research Project Grant (£170K)
  6. Cross NC, Sahota S, Tapper W, Strefford JC (2012) Development and application of a bioinformatic pipeline to identify somatic mutations by exome sequencing. Leukaemia and Lymphoma Research Project Grant (£230K)
  7. Strefford JC, Davies A (2012) FC Receptor analysis in the ROCHE Gallium Trial. ROCHE Pharmaceuticals Project Grant (£130K)
  8. Glennie MJ, Williams T, Johnson PJ, Roghanian A, Strefford JC, Beers S, Reading I (2011) Improving the predictive capacity of in vitro cytokine release assays to reduce animal use and drug attrition. NC3R/SRBI - CRACK-IT (£500K)
  9. Strefford JC, Oscier DG, Packham GP (2011) The genetics and functional characterization of novel genetic lesions in patients with chronic lymphocytic leukaemia. Leukaemia and Lymphoma Research Project Grant (£200K)
  10. Strefford JC, Collins A, Oscier DG, Packham G (2011) The development of next generation sequencing for the analysis of B cell tumours: Understanding disease progression in patients with chronic lymphocytic leukaemia. Wessex Medical Research PhD studentship (£80K)
  11. Pepper C, Baird D, Oscier DG, Strefford JC (2011) Telomere length as a biomarker of genomic instability and predictor of response to treatment. Cancer Research UK - The Biomarkers and Imaging Discovery and Development Committee (£200K)
  12. Strefford JC, Collins A, Oscier DG (2011) The genetic analysis of patients with progressive CLL using next generation sequencing. Kay Kendell Leukaemia Fund Project Grant (£190K)

Group Members (current)
Dr Jonathan Strefford – Reader and Group Leader
Dr Helen Parker – Senior Postdoctoral Research Fellow
(CLL Genomics)
Dr Matthew Rose-Zerilli – Senior Postdoctoral Research Fellow
(CLL Genomics)
Dr Marina Parry – Postdoctoral Research Fellow (SMZL Genomics)
Dr Marta Larrayoz – Postdoctoral Research Fellow (Functional analysis of SF3B1)
Dr Chantal Hargreaves – Postdoctoral Research Fellow (FCGR Genetics)
Dr Chisako Iriyama – Visiting Clinician Scientist (Japan)
Mr Anton Parker – Visiting Scientist (Royal Bournemouth Hospital)
Miss Jade Forster – Graduate Student (CLL Genomics)
Mr Stuart Blakemore – Graduate Student (Functional analysis of SF3B1)

Group Members (past)
Mr Miqdad Rajibali – Welcome Trust PhD Student

Mr Lee Brown – Visiting Masters Student (Cranfield University)

Mr Alistair McIntosh – Visiting Undergraduate Project Student (Birmingham University)
Dr Qian An – Postdoctoral Fellow (now at Portsmouth University)

Academic unit:  Cancer Sciences

Responsibilities

Postgraduate student supervision

2011 Miqdad Rajabaki, MPhil
2010 Victoria Hammond, PhD
2008 Helen Parker, PhD

2007 Lisa Russell, PhD

2005 Timothy Lane, MD

Current

Jade Forster, PhD (2011-2015)
Stuart Blakemore, PhD (2012-2016)

Faculty of Medicine

Member – Faculty Website Strategy Committee
Member – Faculty IT Strategy Group
Member - Postdoctoral Association Committee

Member - Postdoctoral Mentoring Working Group

Member - Divisional Laboratory Committee

National and International Responsibilities

Editorial Board Member – Cancer Genomics and Proteomics

Editorial Board Member – Journal of Hematology
Editorial Board Member – Journal of Carcinogenesis and Mutagenesis

Editorial Board Member – The Open Access Journal of Science
Editorial Board Member – Open Journal of Genomics
Editorial Board Member – Archives of Medical Research



Member – Association of Clinical Cytogenetics

Member – British Society of Human Genetics

Member – British Society of Haematology

Member – American Association for Cancer Research

Member – American Society of Hematology

Member – European Research Initiative on CLL (ERIC)

Co-Chairperson - UK CLL Scientific Workshop
Co-Chairperson – ERIC Novel mutations Project
Executive Member - UK CLL Forum
Member - UK CLL Biobank Research Governence Committee
Member - UK CLL Trials Committee
Member – UK Childhood Leukaemia Research Group



Manuscripts review including - Annals of Clinical Biochemistry, Anticancer Research, Archives of Medical Research, Blood, BioEssays, Bioinformatics, Biotechniques, BMC Gastroenterology, BMC Medical Genomics, BMC Research Notes, British Journal of Cancer, British Journal of Haematology, Cancer Genomics and Proteomics, Cancer Genetics and Cytogenetics, Cancer Therapy, Cancer Therapy and Molecular Biology, Critical Reviews in Oncogenesis, Cytogenetics and Genome Research, Genes Chromosome and Cancer, Heamatologica, Hematological Oncology, Human Molecular Genetics, Journal of Carcinogenesis and Mutagenesis, Journal of Medical Genetics, Journal of Pathology, Laboratory Medicine, Leukemia, Leukemia and Lymphoma, Leukemia Research, Molecular Cytogenetics, Molecular Genetics, Oncogene.

Grant review for - Leukaemia and Lymphoma Research, Medical Research Council (Project grants and Clinical Research Training Fellowship awards), the Kay Kendell Leukaemia Fund (Project grants and Clinical Research Training Fellowship awards), Cancer Research UK, the Association for International Cancer Research (AICR) and CHILDREN with LEUKAEMIA.

Teaching Responsibilities

Faculty of Medicine PhD Programme. Internal examiner, PhD supervisor, project supervisor and marker, lecturer.

Faculty of Medicine Intercalated BMedSci Programme. Project supervisor and marker

University of Southampton Inter-Professional Common Learning. Facilitator.

University of Southampton Bachelor of Medicine BM4. Facilitator.

Contact

Dr Jonathan Strefford
Somers Cancer Research Building
Southampton General Hospital
Mailpoint 824
Tremona Road
Southampton
SO16 6YD

Room Number: SGH//MP824

Telephone: (023) 8120 5246
Facsimile: (023) 8120 5152
Email: jcs@soton.ac.uk