Medicine

Richard Oreffo

DPhil, CBiol FSB

Primary position:
Professor of Musculoskeletal Science

Background

The University of Southampton

Professor Richard Oreffo holds the chair of Musculoskeletal Science and is Co-founder of the Centre for Human Development, Stem Cells and Regeneration. He is also Associate Dean Enterprise for the Faculty of Medicine.

Richard leads the Bone and Joint Research Group based at the Institute of Developmental Sciences, Southampton General Hospital. He has extensive expertise in skeletal biology and the mechanisms involved in skeletal stem cell differentiation.

The Group is focused on understanding bone development and developing strategies to regenerate bone and cartilage using stem cell technology and innovative scaffolds for orthopaedic application. Much of the work is undertaken in multidisciplinary programmes and Richard has published more than 150 peer-reviewed papers, 20 reviews andbook chapters, and holds 5 patents.

In 2001 Richard was recognised with the Maxime Hanns award for collaborative research in Bone Tissue Engineering, appointed to a Senior Lectureship in 2002 and to a Readership and Personal chair in 2004.

In 2008 he was appointed to the Research Council for Health of the Academy of Finland and is a member of the BBSRC Healthy Organism Panel (Deputy Chair), ARC Research Committee and UK National Stem Cell Network Advisory Committee.

Richard serves on the editorial boards of the journals Biomaterials, Tissue Engineering, European Cells and Materials, Regenerative Medicine, Journal of Tissue Engineering and Journal of Tissue Engineering and Regenerative Medicine.  He is on the board of Southampton Asset Management and has consulted for several biotechnology / Pharma companies.

Qualifications

B.Sc. Honours, Biology, University of Liverpool, 1983
D.Phil. Vitamin A and Bone, University of Oxford, 1986

Appointments held

Demonstrator, Oxford Brookes University, Oxford. 1985-1986

Research Fellow, Department of Medicine, Division of Endocrinology,
University of Texas, San Antonio, Texas, USA. 1987-1989

Postdoctoral Research Fellow, Zeneca Pharmaceuticals, Cheshire, UK.1990-1991

Principal Research Scientist, Zeneca Pharmaceuticals, Cheshire, UK.1991-1993

MRC Research Fellow, University of Oxford, Oxford, UK. 1993-1999

Non-Clinical lecturer, University of Southampton, Southampton, UK.1999-2002

Senior lecturer, University of Southampton, Southampton, UK.2002-2004

Reader, University of Southampton, Southampton, UK. 2004

Personal Chair in Musculoskeletal Science. 2004-present

Director of Enterprise – Faculty of Medicine, Health and Life Sciences. 2005-present

Associate Dean (Innovation and Enterprise), Faculty of Medicine, Health and Life Sciences. 2008-2010

Associate Dean Enterprise, Faculty of Medicine. 2010-2011

Associate Dean, International and Enterprise, 2011 - present

Adjunct Professor, King Saud University, Saudi Arabia. 2010-present

Publications

The University of Southampton's electronic library (e-prints)

Article

Lanham, S.A., Bertram, C., Cooper, C. and Oreffo, R.O. (2011) Animal models of maternal nutrition and altered offspring bone structure. European Cells and Materials, 22, 321-332. (PMID:22116650).
McMurray, Rebecca J., Gadegaard, Nikolaj, Tsimbouri, P. Monica, Burgess, Karl V., McNamara, Laura E., Tare, Rahul, Murawski, Kate, Kingham, Emmajayne, Oreffo, Richard O.C. and Dalby, Matthew J. (2011) Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. Nature Materials, 10, (8), 637-644. (doi:10.1038/nmat3058). (PMID:21765399).
El-Serafi, A.T., Wilson, D.I., Roach, H.I. and Oreffo, R.O.C. (2011) Developmental plasticity of human foetal femur-derived cells in pellet culture: self assembly of an osteoid shell around a cartilaginous core. European Cells & Materials, 21, 558-567. (PMID:21710446).
Lanham, Stuart., Fowden, Abby L., Roberts, Carol, Cooper, Cyrus, Oreffo, Richard O.C. and Forhead, Alison J. (2011) Effects of hypothyroidism on the structure and mechanical properties of bone in the ovine fetus. Journal of Endocrinology (doi:10.1530/JOE-11-0138). (PMID:21642376).
de Andres, Maria C., Imagawa, Kei, Hashimoto , Ko, Gonzalez, Antonio, Goldring, Mary B., Roach, Helmtrud I. and Oreffo, Richard O.C. (2011) Suppressors of cytokine signalling (SOCS) are reduced in osteoarthritis. Biochemical and Biophysical Research Communications, 1, (407), 54-59. (PMID:21352802).
Imagawa, Kei, de Andres, M. C., Hashimoto, Ko, Pitt, Dominic, Itobi, Eiji, Goldring, Mary B., Roach, Helmtrud I. and Oreffo, Richard O.C. (2011) The epigenetic effect of glucosamine and a nuclear factor-kappa B (NF-kB)inhibitor on primary human chondrocytes-implications for osteoarthritis. Biochemical and Biophysical Research Communications, 405, (3), 362-367. (doi:10.1016/j.bbrc.2011.01.007). (PMID:21219853). (Submitted).
El-Serafi, Ahmed T., Oreffo, Richard O. and Roach, Helmtrud I. (2011) Epigenetic modifiers influence lineage commitment of human bone marrow stromal cells: Differential effects of 5-aza-deoxycytidine and trichostatin A. Differentiation, 81, (1), 35-41. (doi:10.1111/(ISSN)1432-0436). (PMID:20970916).
Gothard, David, Tare, Rahul S., Mitchell, Peter D., Dawson, Jonathan I. and Oreffo, Richard O.C. (2011) In search of the skeletal stem cell: isolation and separation strategies at the macro/micro scale for skeletal regeneration. Lab on a Chip, 11, (7), 1206-1220. (doi:10.1039/C0LC00575D). (PMID:21350777).
Tare, R.S., Kanczler, J., Aarvold, A., Jones, A.M.H., Dunlop, D.G. and Oreffo, R.O.C. (2010) Skeletal stem cells and bone regeneration: translational strategies from bench to clinic. Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, 224, (12), 1455-1470. (doi:10.1243/09544119JEIM750). (PMID:21287831).
Kanczler, Janos M., Sura, Harpul S., Magnay, Julia, Green, David, Oreffo, Richard O. C., Dobson, Jon P. and El Haj, Alicia J. (2010) Controlled differentiation of human bone marrow stromal cells using magnetic nanoparticle technology. Tissue engineering. Part A, 16, (10), 3241-3250. (doi:10.1089/ten.tea.2009.0638). (PMID:20504072).
Hails, Lauren A., Babister, Jodie C., Inglis, Stefanie, Davis, Sean A., Oreffo, Richard O. and Mann, Stephan (2010) Inhibition of hydroxyapatite nanoparticle-induced osteogenic activity in skeletal cells by adsorption of serum proteins. Small, 6, (18), 1986-1991. (doi:10.1002/smll.201000939). (PMID:20721953).
Kantawong, Fahsai, Burgess, Karl E.V., Jayawardena, Kamburapola, Hart, Andrew, Riehle, Mathias O., Oreffo, Richard O., Dalby, Matthew J. and Burchmore, Richard (2010) Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells. Acta Biomaterialia, 6, (9), 3694-3703. (doi:10.1016/j.actbio.2010.02.038). (PMID:20302977). (Submitted).
McNamara, Laura E., McMurray, Rebecca J., Biggs, Manus J., Kantawong, Fahsai, Oreffo, Richard O. and Dalby, Matthew J. (2010) Nanotopographical control of stem cell differentiation. Journal of Tissue Engineering, 2010, (120623), 120623-[13pp]. (doi:10.4061/2010/120623). (PMID:21350640).
Thomas, Rupert S.W., Mitchell, Peter D., Oreffo, Richard O. and Morgan, Hywel (2010) Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device. [in special issue: Dielectrophoresis] Biomicrofluidics, 4, (2), 022806. (doi:10.1063/1.3406951). (PMID:20697594). (Submitted).
Khan, F, Tare, Rahul S., Kanczler, J.M., Oreffo, Richard O.C. and Bradley, Mark (2010) Strategies for cell manipulation and skeletal tissue engineering using high-throughput polymer blend formulation and microarray techniques. Biomaterials, 31, (8), 2216-2228. (doi:10.1016/j.biomaterials.2009.11.101). (PMID:20056271).
Kanczler, Janos, Ginty, P., White, L., Clarke, N., Howdle, S., Shakesheff, K. and Oreffo, R. (2010) The effect of the delivery of vascular endothelial growth factor and bone morphogenic protein-2 to osteoprogenitor cell populations on bone formation. Biomaterials, 31, (6), 1241-1250. (doi:10.1016/j.biomaterials.2009.10.059).
Forristal, Catherine E., Wright, Kate L., Hanley, Neil A., Oreffo, Richard O.C. and Houghton, Franchesca D. (2010) Hypoxia inducible factors regulate pluripotency and proliferation in human proliferation in human embryonic stem cells cultured at reduced oxygen tensions. Reproduction, 139, (1), 85-97. (doi:10.1530/REP-09-0300).
Lanham, S.A., Roberts, C., Hollingworth, T., Sreekumar, R., Elahi, M.M., Cagampang, F.R., Hanson, M.A. and Oreffo, R.O.C. (2009) Maternal high-fat diet: effects on offspring bone structure. Osteoporosis International, 1-12. (doi:10.1007/s00198-009-1118-4).
Berry, C.C., Harianawalw, H., Loebus, J., Oreffo, Richard O.C. and de la Fuente, J. (2009) Enhancement of human bone marrow cell uptake of quantum dots using tat peptide. Current Nanoscience, 5, (4), 390-395. (doi:10.2174/157341309789378122).
Hashimoto, Ko, Oreffo, Richard O.C., Gibson, Marc B., Goldring, Mary B. and Roach, Helmstrud I. (2009) DNA demethylation at specific CpG sites in the IL1B promoter in response to inflammatory cytokines in human articular chondrocytes. Arthritis and Rheumatism, 60, (11), 3303-3313. (doi:10.1002/art.24882). (PMID:19877066).
Biggs, Manus J., Richards, R. Geoff, Gadegaard, Nikolaj, Wilkinson, Chris D.W., Oreffo, Richard O. and Dalby, Matthew J. (2009) The use of nanoscale topography to modulate the dynamics of adhesion formation in primary osteoblasts and ERK/MAPK signalling in STRO-1+enriched skeletal stem cells. Biomaterials, 30, (28), 5094-5103. (doi:10.1016/j.biomaterials.2009.05.049). (PMID:19539986).
Kantawong, Fahsai, Burgess, Karl E.V., Jayawardena, Kamburapola, Hart, Andrew, Burchmore, Richard J., Gadegaard, Nikolaj, Oreffo, Richard O.C. and Dalby, Matthew J. (2009) Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling. Biomaterials, 30, (27), 4723-4732. (doi:10.1016/j.biomaterials.2009.05.040). (PMID:19560200).
Mirmalek-Sani, S.H., Stokes, P.J., Tare, R.S., Ralph, E.J., Inglis, S., Hanley, N.A., Houghton, F.D. and Oreffo, R.O. (2009) Derivation of a novel undifferentiated human fetal phenotype in serum-free cultures with BMP-2. Journal of Cellular and Molecular Medicine, 13, (9B), 3541-3555. (doi:10.1111/j.1582-4934.2009.00742.x).
Kanczler, Janos M., Mirmalek-Sani, Sayed-Hadi, Hanley, Neil A., Ivanov, Alexander L., Barry, John J.A., Upton, Claire, Shakesheff, Kevin M., Howdle, Steven M., Antonov, Eugeuni N., Bagratashvili, Victor N., Popov, Vladimir K. and Oreffo, Richard O.C. (2009) Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds. Acta Biomaterialia, 5, (6), 2063-2071. (doi:10.1016/j.actbio.2009.03.010).
Sengers, B.G., Please, C.P., Taylor, M. and Oreffo, R.O.C. (2009) Experimental–computational evaluation of human bone marrow stromal cell spreading on trabecular bone structures. Annals of Biomedical Engineering, 37, (6), 1165-1176. (doi:10.1007/s10439-009-9676-3).
Sjostrom, Terje, Dalby, Matthew J., Hart, Andrew, Tare, Rahul S., Oreffo, Richard O.C. and Su, Bo (2009) Fabrication of pillar-like titania nanostructures on titanium and their interactions with human skeletal stem cells. Acta Biomaterials, 5, (5), 1433-1441. (doi:10.1016/j.actbio.2009.01.007).
Babister, Jodie C., Hails, Lauren A, Oreffo, Richard O.C., Davis, Sean and Mann, Stephen (2009) The effect of pre-coating human bone marrow stromal cells with hydroxyapatite/amino acid nanoconjugates on osteogenesis. Biomaterials, 30, (18), 3174-3182. (doi:10.1016/j.biomaterials.2009.02.024 ).
Green, David W., Bolland, Benjamin J.R., Kanczler, Janos M., Lanham, Stuart A., Walsh, Dominic, Mann, Stephen and Oreffo, Richard O.C. (2009) Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites. Biomaterials, 30, (10), 1918-1927. (doi:10.1016/j.biomaterials.2008.12.052 ).
Morgan, Suzanne M., Ainsworth, Ben J., Kanczler, Janos M. , Babister, Jodie C., Chaudhuri, Julian B. and Oreffo, Richard O. C. (2009) Formation of a human-derived fat tissue layer in PDLLGA hollow fibre scaffolds for adipocyte tissue engineering. Biomaterials, 30, (10), 1910-1917. (doi:10.1016/j.biomaterials.2008.12.033).
Tare, Rahul S., Khan, Ferdous, Tourniaire, Guilhem, Morgan, Suzanne M., Bradley, Mark and Oreffo, Richard O.C. (2009) A microarray approach to the identification of polyurethanes for the isolation of human skeletal progenitor cells and augmentation of skeletal cell growth. Biomaterials, 30, (6), 1045-1055. (doi:10.1016/j.biomaterials.2008.10.038).
Khan, Ferdous, Tare, Rahul S., Oreffo, Richard O.C. and Bradley, Mark (2009) Versatile biocompatible polymer hydrogels: scaffolds for cell growth. Angewandte Chemie International Edition, 48, (5), 978-982. (doi:10.1002/anie.200804096). (PMID:19115339).
Collins, Andrew M., Skaer, Nick J.V., Gheysens, Tom, Knight, David, Bertram, Caroline, Roach, Helmtrud I., Oreffo, Richard O.C., Von-Aulock, Sonja, Baris, Teodora, Skinner, John and Mann, Stephen (2009) Bone like resorbable silk based scaffolds for load bearing osteoregenerative applications. Advanced Materials, 20, (1), 75-78. (doi:10.1002/adma.200802239).
Hails, Lauren A., Babister, Jodie C., Oreffo, Richard O.C., Davis, Sean A. and Mann, Stephen (2009) Cell surface coatings of hydroxyapatite/amino acid nanoconjugates stimulate osteogenesis of human bone marrow stromal cells both in vitro and in vivo. Advanced Functional Materials (Submitted).
Bolland, Benjamin J.R.F., New, Andrew M.R., Madabhushi, Gopal, Oreffo, Richard O.C. and Dunlop, Douglas G. (2008) The role of vibration and drainage in femoral impaction bone grafting. Journal of Arthroplasty, 23, (8), 1-8. (doi:10.1016/j.arth.2007.10.011). (PMID:18534472).
Biggs, M.J.P., Richards, R.G., McFarlane, S., Wilkinson, C.D.W., Oreffo, R.O.C. and Dalby, M.J. (2008) Adhesion formation of primary human osteoblasts and the functional response of mesenchymal stem cells to 330nm deep microgrooves. Journal of the Royal Society Interface, 5, (27), 1231-1242. (doi:10.1098/rsif.2008.0035).
Biggs, Manus J., Richards, R. Geoff, Gadegaard, Nikolaj, McMurray, Rebecca J., Affrossman, Stanley, Wilkinson, Chris D.W., Oreffo, Richard O.C. and Dalby, Mathew J. (2008) Interaction with nanoscale topography: Adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. Journal of Biomedical Materials Research Part A, 91A, (1), 195-208. (doi:10.1002/jbm.a.32196).
Dalby, Matthew J., Andar, Abhay, Nag, Abhijit, Affrossman, Stanley, Tare, Rahul, McFarlane, Sara and Oreffo, Richard O.C. (2008) Genomic expression of mesenchymal stem cells to altered nanoscale topographies. Journal of the Royal Society Interface, 5, (26), 1055-1065. (doi:10.1098/rsif.2008.0016).
Bolland, B.J., Kanczler, J.M., Ginty, P.J., Howdle, S.M., Shakesheff, K.M., Dunlop, D.G. and Oreffo, R.O. (2008) The application of human bone marrow stromal cells and poly(dl-lactic acid) as a biological bone graft extender in impaction bone grafting. Biomaterials, 29, (22), 3221-3227. (doi:10.1016/j.biomaterials.2008.04.017).
Dawson, Jonathan I., Wahl, Denys A., Lanham, Stuart A., Kanczler, Janos M., Czernuszka, Jan T. and Oreffo, Richard O.C. (2008) Development of specific collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells. Biomaterials, 29, (21), 3105-3116. (doi:10.1016/j.biomaterials.2008.03.040).
Pollintine, P., Cooper, I., Anderson, H.L., Green, L.R., Cooper, C., Lanham, S.A., Oreffo, R.O.C. and Dolan, P. (2008) The influence of early nutritional compromise on bone structure and strength. Journal of Bone and Joint Surgery, 90-B, (Supp II), p.392.
Bolland, B.J., Kanczler, J.M., Dunlop, D.G. and Oreffo, R.O. (2008) Development of in vivo muCT evaluation of neovascularisation in tissue engineered bone constructs. Bone, 43, (1), 195-202. (doi:10.1016/j.bone.2008.02.013).
Pollintine, P., Barrett, G.W., Millais, I.E., Green, L.R., Cooper, C., Lanham, S.A., Oreffo, R.O.C. and Dolan, P. (2008) Early nutritional compromise affects the mechanical properties of sheep spines. Journal of Bone and Joint Surgery, 90B, (Supplement 2), 373-374.
Tare, Rahul S., Babister, Jodie C., Kanczler, Janos and Oreffo, Richard O.C. (2008) Skeletal stem cells: phenotype, biology and environmental niches informing tissue regeneration. [in special issue: Stem Cells and Progenitors in Organ Maintenance, Regeneration and Replacement: the Role of Hormones and Growth Factors in Health and Disease] Molecular and Cellular Endocrinology, 288, (1-2), 11-21. (doi:10.1016/j.mce.2008.02.017). (PMID:18395331).
Bagratashvili, Victor, Antonov, E.N., Howdle, S.M., Kanczler, J.M., Mirmalek-Sani, S., Popov, V.K., Oreffo, R.O. and Upton, C., Katashev, Alexei, Dekhtyar, Yuri and Spigulis, Janis (eds.) (2008) Osteogenesis on surface selective laser sintered bioresorbable scaffolds. 14th nordic-baltic conference on biomedical engineering and medical physics, 20, (2), 12-15. (doi:10.1007/978-3-540-69367-3_4).
Dawson, Jonathan I. and Oreffo, Richard O.C. (2008) Bridging the regeneration gap: Stem cells, biomaterials and clinical translation in bone tissue engineering. Archives of Biochemistry and Biophysics, 473, (2), 124-31. (doi:10.1016/j.abb.2008.03.024).
Kanczler, J.M. and Oreffo, R.O.C. (2008) Osteogenesis and angiogenesis: the potential for engineering bone. European Cells & Materials, 15, 100-114.
Kanczler, J.M., Ginty, P.J., Barry, J.J., Clarke, N.M., Howdle, S.M., Shakesheff, K.M. and Oreffo, R.O. (2008) The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation. Biomaterials, 29, (12), 1892-1900. (doi:10.1016/j.biomaterials.2007.12.031).
Tare, Rahul S., Townsend, Paul A., Packham, Graham, Inglis, Stefanie and Oreffo, Richard O.C. (2008) BCL-2 associated athanogene-1 (BAG-1): A transcriptional regulator mediating chondrocyte survival and differentiation during endochondral ossification. Bone, 42, (1), 113-128. (doi:10.1016/j.bone.2007.08.032). (PMID:17950682).
Babister, Jodie C., Tare, Rahul S., Green, David W., Inglis, Stefanie, Mann, Stephen and Oreffo, RRichard O.C. (2008) Genetic manipulation of human mesenchymal progenitors to promote chondrogenesis using “bead-in-bead” polysaccharide capsules. Biomaterials, 29, (1), 58-65. (doi:10.1016/j.biomaterials.2007.09.006). (PMID:17897711).
Lanham, S.A., Roberts, C., Cooper, C. and Oreffo, R.O. (2008) Intrauterine programming of bone. Part 1: Alteration of the osteogenic environment. Osteoporosis International, 19, (2), 147-156. (doi:10.1007/s00198-007-0443-8).
Kantawong, Fahsai, Burchmore, Richard, Gadegaard, Nikolaj, Oreffo, Richard O.C. and Dalby, Matthew J. (2008) Proteomic analysis of human osteoprogenitor response to disordered nanotopography. Journal of the Royal Society Interface, 6, (40), 1075-1086. (doi:10.1098/​rsif.2008.0447).
Kantawong, Fahsai, Burchmore, Richard, Wilkinson, Chris D.W., Oreffo, Richard O.C. and Dalby, Matthew J. (2008) Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance. Acta Biomaterialia, 5, (4), 1137-1146. (doi:10.1016/j.actbio.2008.11.001).
Gribbon, Christopher, Channon, Kevin J., Zhang, Wenjian, Banwell, Elenor F., Bromley, Elizabeth H.C., Chaudhuri, Julian B., Oreffo, Richard O.C. and Woolfson, Derek N. (2008) Magic-wand: a single, designed peptide that assembles to stable, ordered helical fibres. Biochemistry, 47, (39), 10365-10371. (doi:10.1021/bi801072s).
MacArthur, Ben D., Tare, Rahul S., Murawski, Kate and Oreffo, Richard O.C. (2008) Identification of candidate regulators of multipotency in human skeletal progenitor cells. Biochemical and Biophysical Research Communications, 377, (1), 68-72. (doi:10.1016/j.bbrc.2008.09.084).
Lanham, S.A., Roberts, C., Perry, M.J., Cooper, C. and Oreffo, R.O. (2008) Intrauterine programming of bone. Part 2: Alteration of skeletal structure. Osteoporosis International, 19, (2), 157-167. (doi:10.1007/s00198-007-0448-3).
MacArthur, Ben D., Please, Colin P. and Oreffo, Richard O.C. (2008) Stochasticity and the molecular mechanisms of induced pluripotency. PLoS ONE, 3, (8), e3086. (doi:10.1371/journal.pone.0003086).
Sengers, Bram G., Please, Colin P. and Oreffo, Richard O.C. (2007) Experimental characterization and computational modelling of two-dimensional cell spreading for skeletal regeneration. Journal of the Royal Society Interface, 4, (17), 1107-1117. (doi:10.1098/rsif.2007.0233).
Morgan, S.M., Tilley, S., Perera, S., Ellis, M.J., Kanczler, J., Chaudhuri, J.B. and Oreffo, R.O. (2007) Expansion of human bone marrow stromal cells on poly-(DL-lactide-co-glycolide) (PDL LGA) hollow fibres designed for use in skeletal tissue engineering. Biomaterials, 28, (35), 5332-5343. (doi:10.1016/j.biomaterials.2007.08.029).
Heyde, Mieke, Partridge, Kris A., Howdle, Steven M., Oreffo, Richard O.C., Garnett, Martin C. and Shakesheff, Kevin M. (2007) Development of a slow non-viral DNA release system from PDLLA scaffolds fabricated using a supercritical CO2 technique. Biotechnology & Bioengineering, 98, (3), 679-693. (doi:10.1002/bit.21446).
Dalby, Matthew J., Gadegaard, Nikolaj, Tare, Rahul, Andar, Abhay, Riehle, Mathis O., Herzyk, Pawel, Wilkinson, Chris D.W. and Oreffo, Richard O.C. (2007) The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nature Materials, 6, 7pp. (doi:10.1038/nmat2013).
Berry, C.C., Curtis, A.S.G., Oreffo, R.O.C., Agheli, H.D.S. and Sutherland, D.S. (2007) Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates. IEEE Transactions on Nanobioscience, 6, (3), 201-209. (doi:10.1109/TNB.2007.903457).
Gonzalez-McQuire, R., Green, D.W., Partridge, K.A., Oreffo, R.O.C., Mann, S. and Davis, S.A. (2007) Coating of human mesenchymal cells in 3D culture with bioinorganic nanoparticles promotes osteoblastic differentiation and gene transfection. Advanced Materials, 19, (17), 2236-2240. (doi:10.1002/adma.200602770).
Hollingworth, T., Lanham, S.A., Roberts, C., Hanson, M., Elahi, M., Cagampang, F., Cooper, C. and Oreffo, R.O.C. (2007) Effects of maternal-dietary cholesterol on offspring bone development. Early Human Development, 83, (Supplement 1), S155-S156. (doi:10.1016/S0378-3782(07)70428-0).
Xu, Yunhe, Mirmalek-Sani, Sayed-Hadi, Lin, Feng, Zhang, Junlong and Oreffo, Richard O.C. (2007) Adipocyte differentiation induced using nonspecific siRNA controls in cultured human mesenchymal stem cells. RNA, 13, (8), 1179-1183. (doi:10.1261/rna.527207).
Tare, R.S., Townsend, P.A., Packham, G.K., Inglis, S. and Oreffo, R.O.C. (2007) BCL-2-associated athanogene-1: A transcriptional regulator mediating chondrocyte survival and differentiation during endochondral ossification. Journal of Bone and Mineral Research, 22, (7), p.1129.
Tare, R., Khan, F., Tourniaire, G., Morgan, S. M., Bradley, M. and Oreffo, R. O. C. (2007) Selection of human mesenchymal osteoprogenitors from bone marrow stromal cell populations using polymer microarrays. Tissue Engineering, 13, (7), p.1754.
Pound, Jodie C., Green, David W., Roach, Helmtrud I., Mann, Stephen and Oreffo, Richard O.C. (2007) An ex vivo model for chondrogenesis and osteogenesis. Biomaterials, 28, (18), 2839-2849. (doi:10.1016/j.biomaterials.2007.02.029).
Bolland, B.J.R.F, New, A.M.R., Madabhushi, S.P.G., Oreffo, R.O.C. and Dunlop, D.G. (2007) Vibration-assisted bone-graft compaction in impaction bone grafting of the femur. Journal of Bone and Joint Surgery: British Volume, 89-B, (5), 686-692. (doi:10.1302/0301-620X.89B5.18695).
Bolland, B.J.R.F, Tilley, S., New, A.M.R., Dunlop, D.G. and Oreffo, R.O.C. (2007) Adult mesenchymal stem cells and impaction grafting – a new clinical paradigm shift. Expert Review of Medical Devices, 4, (3), 393-404. (doi: 10.1586/17434440.4.3.393).
Sengers, Bram G., Taylor, Mark, Please, Colin P. and Oreffo, Richard O.C. (2007) Computational modelling of cell spreading and tissue regeneration in porous scaffolds. Biomaterials, 28, (10), 1926-1940. (doi:10.1016/j.biomaterials.2006.12.008).
Heyde, Mieke, Partridge, Kris A., Oreffo, Richard O.C., Howdle, Steven M., Shakesheff, Kevin M. and Garnett, Martin C. (2007) Gene therapy used for tissue engineering applications. Journal of Pharmacy and Pharmacology, 59, (3), 329-350.
Kanczler, J.M., Barry, J., Ginty, P., Howdle, S.M., Shakesheff, K.M. and Oreffo, R.O. (2007) Supercritical carbon dioxide generated vascular endothelial growth factor encapsulated poly(DL-lactic acid) scaffolds induce angiogenesis in vitro. Biochemical and biophysical research communications, 352, (1), 135-141.
Bolland, B.J., Tilley, S., New, A.M., Dunlop, D.G. and Oreffo, R.O. (2007) Adult mesenchymal stem cells and impaction grafting: a new clinical paradigm shift. Expert Review of Medical Devices, 4, (3), 393-404.
Lanham, S.A., Roberts, C., Perry, M.J., Oreffo, R.O.C. and Cooper, C. (2007) Intrauterine programming of bone-deleterious effects of a low protein diet in late adulthood on the osteogenic environment. Journal of Bone and Mineral Research, 22, (7)
Bolland, B.J., Kanczler, J., Ginty, P., Shakesheff, K.M., Dunlop, D.G. and Oreffo, R.O.C. (2007) Poly lactic acid scaffold augmented with human bone marrow stromal cells as a bone graft extender in impaction brone grafting. Tissue Engineering, 13, (7), p.1742.
Dalby, M.J., Gadegaard, N., Curtis, A.S. and Oreffo, R.O. (2007) Nanotopographical control of human osteoprogenitor differentiation. Current Stem Cell Research & Therapy, 2, (2), 129-138. (doi:10.2174/157488807780599220).
Tare, R.S., Forsey, R.W., Chaudhuri, J.B. and Oreffo, R.O.C. (2007) Engineering carilaginous explants using human stro -1+ pregenitors, alginate scaffolds and perfusion bioreactors. Tissue Engineering, 13, (7)
Hart, Andrew, Gadegaard, Nikolaj, Wilkinson, Chris D., Oreffo, Richard O. and Dalby, Matthew J. (2007) Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography. Journal of Materials Science: Materials in Medicine, 18, (6), 1211-1218. (doi:10.1007/s10856-007-0157-7).
Pound, Jodie C., Green, David W., Chaudhuri, Julian B., Mann, Stephen, Roach, Helmtrud I. and Oreffo, Richard O.C. (2006) Strategies to promote chondrogenesis and osteogenesis from human bone marrow cells and articular chondrocytes encapsulated in polysaccharide templates. Tissue Engineering, 12, (10), 2789-2799. (doi:10.1089/ten.2006.12.2789).
Tilley, Simon, Bolland, Benjamin J.R.F., Partridge, Kris, New, Andrew M.R., Latham, Jeremy M., Dunlop, Douglas G. and Oreffo, Richard O.C . (2006) Taking tissue-engineering principles into theater: augmentation of impacted allograft with human bone marrow stromal cells. Regenerative Medicine, 1, (5), 685-692. (doi:10.2217/17460751.1.5.685).
Bolland, Benjamin J.R.F., Partridge, Kris, Tilley, Simon, New, Andrew M.R., Dunlop, Douglas G. and Oreffo, Richard O.C. (2006) Biological and mechanical enhancement of impacted allograft seeded with human bone marrow stromal cells: potential clinical role in impaction bone grafting. Regenerative Medicine, 1, (4), 457-467. (doi:10.2217/17460751.1.4.457).
Tare, R.S., Townsend, P.A., Packham, G. and Oreffo, R.O.C. (2006) Insight into the function of Bcl-2-associated athanogene-1 anti-apoptotic protein during chondrocyte development and differentiation. Journal of Bone and Mineral Research, 21, (7), p.1150.
Xu, Y., Mirmalek-Sani, S.-H., Yang, X., Zhang, J. and Oreffo, R.O.C. (2006) The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells. Experimental Cell Research, 312, (10), 1856-1864. (doi:10.1016/j.yexcr.2006.02.016).
Ginty, Patrick J., Howard, Daniel, Rose, Felicity R.A.J., Whitaker, Martin J., Barry, John J.A., Tighe, Patrick, Mutch, Stacey R., Serhatkulu, Gulay, Oreffo, Richard O.C., Howdle, Steven M. and Shakesheff, Kevin M. (2006) Mammalian cell survival and processing in supercritical CO(2). Proceedings of the National Academy of Sciences, 103, (19), 7426-7431. (doi:10.1073/pnas.0508895103).
Oreffo, R.O. (2006) Bone tissue engineering - from cells, mice to man - bridging the gap. Bio materialien, 7, (Supplement 1)
Yang, Xuebin B., Webb, Derek, Blaker, Jonny, Boccaccini, Aldo R., Maquet, Veronique, Cooper, Cyrus and Oreffo, Richard O.C. (2006) Evaluation of human bone marrow stromal cell growth on biodegradable polymer/Bioglass composites. Biochemical and Biophysical Research Communications, 342, (4), 1098-1107. (doi:10.1016/j.bbrc.2006.02.021).
Yang, X.B., Green, D.W., Roach, H. I., Anderson, H.C., Howdle, S.M., Shakesheff, K.M. and Oreffo, R.O.C. (2006) The effect of an admix of bone morphogenetic proteins on human osteoprogenitor activity in vitro and in vivo. Tissue Engineering, 12, (4), 1002-1003. (doi:10.1089/ten.2006.12.983).
MacArthur, Ben D., Tare, Rahul S., Please, Colin P., Prescott, Philip and Oreffo, Richard O.C. (2006) A non-invasive method for in situ quantification of subpopulation behaviour in mixed cell culture. Journal of the Royal Society Interface, 3, (6), 63-69. (doi:10.1098/rsif.2005.0080).
Dalby, M.J., McCloy, D., Robertson, M., Agheli, H.D., Affrossman, S. and Oreffo, R.O. (2006) Osteoprogenitor response to semi-ordered and random nanotopographies. Biomaterials, 27, (15), 2980-2987. (doi:10.1016/j.biomaterials.2006.01.010).
Berry, C.C., Dalby, M.J., Oreffo, R.O., McCloy, D. and Affrosman, S. (2006) The interaction of human bone marrow cells with nanotopographical features in three dimensional constructs. Journal of Biomedical Materials Research Part A, 79, (2), 431-439.
Yamada, N., Cheung, K.S.C., Tilley, S., Clarke, N.M.P., Oreffo, R.O.C., Kokobun, S., Bronner, F. and Roach, H.I. (2006) Does epigenetics play a role in the pathology of osteoarthritis? Journal of Bone and Joint Surgery, 88-B, (Supplement III), p.403.
Kanczler, J., Barry, J., Ginty, P., Sebald, W., Howdle, S., Shakesheff, K. and Oreffo, R.O. (2006) New scaffolds for skeletal regeneration stimulating angiogenesis and osteogenesis. Journal of Bone and Mineral Research, 21, (7), p.1153.
MacArthur, B.D., Tare, R.S., Please, C.P., Prescott, P. and Oreffo, R.C. (2006) A Non-invasive method for in situ Quantification of Subpopulation behaviour in Mixed Cell culture. Journal of the Royal Society Interface, 3, (6), 63-69. (doi:10.1098/rsif.2005.0080).
Silva, M.M., Cyster, L.A., Barry, J.J., Yang, X.B., Oreffo, R.O., Grant, D.M., Scotchford, C.A., Howdle, S.M., Shakesheff, K.M. and Rose, F.R. (2006) The effect of anisotropic architecture on cell and tissue infiltration into tissue engineering scaffolds. Biomaterials, 27, (35), 5909-5917. (doi:10.1016/j.biomaterials.2006.08.010).
Tilley, Simon, Bolland, Benjamin J.R.F., Partridge, Kris, New, Andrew M.R., Latham, Jeremy M., Dunlop, Douglas G. and Oreffo, Richard O.C. (2006) Taking tissue engineering principles into theatre: augmentation of impacted allograft with human bone marrow stromal cells. Regenerative Medicine, 1, (5), 685-692. (doi:10.2217/17460751.1.5.685).
Partridge, K., Heyde, M., Garnett, M., Howdle, S. M., Shakesheff, K. and Oreffo, R. (2006) Cloning and expression of human BMP-2 potential for use in skeletal tissue engineering. Journal of Bone and Mineral Research, 21, (7)
Ginty, Patrick J., Howard, Daniel, Rose, Felicity R.A.J., Whitaker, Martin J., Barry, John J.A., Tighe, Patrick, Mutch, Stacey R., Serhatkulu, Gulay, Oreffo, Richard O.C., Howdle, Steven M. and Shakesheff, Kevin M. (2006) Mammalian cell survival and processing in supercritical CO2. Proceedings of the National Academy of Sciences of the USA, 103, (19), 7426-7431. (doi:10.1073/pnas.0508895103).
Roach, H.I., Inglis, S., Partridge, K., Oreffo, R. and Clarke, N.M.P. (2006) Can epigenetic changes in DNA metylations explain the altered gene expression in osteoarthritis? Journal of Bone and Joint Surgery, 88-B, (Supplement III), 385-386.
Roach, Helmtrud I., Yamada, Norikazu, Cheung, Kelvin S.C., Tilley, Simon, Clarke, Nicholas M.P., Oreffo, Richard O.C., Kokubun, Shoichi and Bronner, Felix (2005) Association between the abnormal expression of matrix-degrading enzymes by human osteoarthritic chondrocytes and demethylation of specific CpG sites in the promoter regions. Arthritis & Rheumatism, 52, (10), 3110-3124. (doi:10.1002/art.21300).
Tare, Rahul S., Howard, Daniel, Pound, Jodie C., Roach, Helmtrud I. and Oreffo, Richard O.C. (2005) Tissue engineering strategies for cartilage generation - micromass and three dimensional cultures using human chondrocytes and a continuous cell line. Biochemical and Biophysical Research Communications, 333, (2), 609-621. (doi:10.1016/j.bbrc.2005.05.117).
Mirmalek-Sani, S.H., Roach, H.I., Hanley, N.A., Wilson, D.I. and Oreffo, R.O.C. (2005) Characterization and plasticity of human fetal-derived cartilage cells: implications for skeletal tissue regeneration. International Journal of Experimental Pathology, 86, (3), A18-A19. (doi:10.1111/j.0959-9673.2005.00426.x).
Oreffo, Richard O.C., Cooper, Cyrus, Mason, Christopher and Clements, Mark (2005) Mesenchymal stem cells: lineage, plasticity and skeletal therapeutic potential. Stem Cell Reviews and Reports, 1, (2), 169-178. (doi:10.1385/SCR:1:2:169).
Mirmalek-Sani, Sayed-Hadi, Tare, Rahul S., Morgan, Suzanne M., Roach, Helmtrud I., Wilson, David I., Hanley, Neil A. and Oreffo, Richard O.C. (2005) Characterization and multipotentiality of human fetal femur-derived cells: implications for skeletal tissue regeneration. Stem Cells, 24, (4), 1042-1053. (doi:10.1634/stemcells.2005-0368).
MacArthur, Ben D. and Oreffo, Richard O. C. (2005) Bridging the gap. Nature, 433, (7021), 19. (doi:10.1038/433019a).
Gonzalez-McQuire, Rosanna, Green, David, Walsh, Dominic, Hall, Simon, Chane-Ching, Jean-Yves, Oreffo, Richard O.C. and Mann, Stephen (2005) Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating. Biomaterials, 26, (33), 6652-6656. (doi:10.1016/j.biomaterials.2005.04.037).
Dalby, Matthew John, McCloy, David, Robertson, Mary, Wilkinson, Christopher D.W. and Oreffo, Richard O.C. (2005) Osteoprogenitor response to defined topographies with nanoscale depths. Biomaterials, 27, (8), 1306-1315. (doi:10.1016/j.biomaterials.2005.08.028).
Green, D.W., Leveque, I., Walsh, D., Howard, D., Yang, X., Partridge, K., Mann, S. and Oreffo, R.O.C. (2005) Biomineralized polysaccharide capsules for encapsulation, organization, and delivery of human cell types and growth factors. Advanced Functional Materials, 15, (6), 917-923. (doi:10.1002/adfm.200400322).
MacArthur, B.D., Please, C.P., Taylor, M. and Oreffo, R.O.C. (2004) Mathematical modelling of skeletal repair. Biochemical and Biophysical Research Communications, 313, (4), 825-833. (doi:10.1016/j.bbrc.2003.11.171).
Partridge, Kris A. and Oreffo, Richard O. C. (2004) Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies. Tissue Engineering, 10, (1-2), 295-307.
Rose, F.R.A.J., Hou, Q. and Oreffo, R.O.C. (2004) Delivery systems for bone growth factors - the new players in skeletal regeneration. Journal of Pharmacy and Pharmacology, 56, (4), 415-427. (doi:10.1211/0022357).
Yang, X.B., Bhatnagar, R.S., Li, S. and Oreffo, R.O.C. (2004) Biomimetic collagen scaffolds for human bone cell growth and differentiation. Tissue Engineering, 10, (7), 1148-1159. (doi:10.1089/ten.2004.10.1148).
Green, D., Walsh, D., Yang, X. B., Mann, S. and Oreffo, R. O. C. (2004) Stimulation of human bone marrow stromal cells using growth factor encapsulated calcium carbonate porous microspheres. Journal of Materials Chemistry, 14, (14), 2206-2212. (doi:10.1039/b400486h).
Oreffo, R.O.C. (2004) Growth factors for skeletal reconstruction and fracture repair. Current Opinion in Investigational Drugs, 5, (4), 419-423.
Yang, Xuebin B., Whitaker, Martin J., Sebald, Walter, Clarke, Nicholas, Howdle, Steven M., Shakesheff, Kevin M. and Oreffo, Richard O.C. (2004) Human osteoprogenitor bone formation using encapsulated bone morphogenetic protein 2 in porous polymer scaffolds. Tissue Engineering, 10, (7), 1037-1045. (doi:10.1089/ten.2004.10.1037).
Smith, James O., Oreffo, Richard O.C., Clarke, Nicholas M.P. and Roach, Helmtrud I. (2003) Changes in the antiangiogenic properties of articular cartilage in osteoarthritis. Journal of Orthopaedic Science, 8, (6), 849-857. (doi:10.1007/s00776-003-0717-8).
Oreffo, Richard O.C., Lashbrooke, Benjamin, Roach, Helmtrud I., Clarke, Nicholas M. and Cooper, Cyrus (2003) Maternal protein deficiency affects mesenchymal stem cell activity in the developing offspring. Bone, 33, (1), 100-107. (doi:10.1016/S8756-3282(03)00166-2).
Roach, Helmtrud I., Mehta, Gautam, Oreffo, Richard O.C., Clarke, Nicholas M.P. and Cooper, Cyrus (2003) Temporal analysis of rat growth plates: cessation of growth with age despite presence of a physis. Journal of Histochemistry and Cytochemistry, 51, (3), 373-383.
Yang, Xuebin, Tare, Rahul S., Partridge, Kris A., Roach, Helmtrud, Clarke, Nicholas M.P., Howdle, Steven M., Shakesheff, Kevin M. and Oreffo, Richard O.C. (2003) Induction of human osteoprogenitor chemotaxis, proliferation, differentiation, and bone formation by osteoblast stimulating factor-1/pleiotrophin: osteoconductive biomimetic scaffolds for tissue engineering. Journal of Bone and Mineral Research, 18, (1), 47-57. (doi:10.1359/jbmr.2003.18.1.47).
Green, D.W., Howard, D., Yang, X., Kelly, M. and Oreffo, R.O.C. (2003) Natural marine sponge fiber skeleton: a biomimetic scaffold for human osteoprogenitor cell attachment, growth, and differentiation. Tissue Engineering, 9, (6), 1159-1166.
Hall, S. R., Walsh, D., Green, D., Oreffo, R. O. C. and Mann, S. (2003) A novel route to highly porous bioactive silica gels. Journal of Materials Chemistry, 13, (2), 186-190. (doi:10.1039/b209300f).
Salem, A. K., Rose, F. R. A. J., Oreffo, R. O. C., Yang, X. B., Davies, M. C., Mitchell, J. R., Roberts, C. J., Stolnik-Trenkic, S., Tendler, S. J. B., Williams, P. M. and Shakesheff, K. M. (2003) Porous polymer and cell composites that self-assemble in situ. Advanced Materials, 15, (3), 210-213. (doi:10.1002/adma.200390047).
Yang, X.B., Green, D.W., Roach, H.I., Clarke, N.M.P., Anderson, H.C., Howdle, S.M., Shakesheff, K.M. and Oreffo, R.O.C. (2003) Novel osteoinductive biomimetic scaffolds stimulate human osteoprogenitor activity: implications for skeletal repair. Connective Tissue Research, 44, (Supplement 1), 312-317. (doi:10.1080/713713599).
Mehta, G., Roach, H.I., Langley-Evans, S., Taylor, P., Reading, I., Oreffo, R.O.C., Aihie-Sayer, A., Clarke, N.M.P. and Cooper, C. (2002) Intrauterine exposure to a maternal low protein diet reduces adult bone mass and alters growth plate morphology in rats. Calcified Tissue International, 71, (6), 493-498. (doi:10.1007/s00223-001-2104-9).
Tare, Rahul S., Oreffo, Richard O.C., Sato, Kenzo, Rauvala, Heikki, Clarke, Nicholas M.P. and Roach, Helmtrud I. (2002) Effects of targeted overexpression of pleiotrophin on postnatal bone development. Biochemical and Biophysical Research Communications, 298, (3), 324-332. (doi:10.1016/S0006-291X(02)02456-7).
Tare, Rahul S., Oreffo, Richard O.C., Clarke, Nicholas M.P. and Roach, Helmtrud I. (2002) Pleiotrophin/Osteoblast-stimulating factor 1: dissecting its diverse functions in bone formation. Journal of Bone and Mineral Research, 17, (11), 2009-2020. (doi:10.1359/jbmr.2002.17.11.2009).
Howard, Daniel, Partridge, Kris, Yang, Xuebin, Clarke, Nicholas M.P., Okubo, Yasunori, Bessho, Kazuhisa, Howdle, Steven M., Shakesheff, Kevin M. and Oreffo, Richard O.C. (2002) Immunoselection and adenoviral genetic modulation of human osteoprogenitors: in vivo bone formation on PLA scaffold. Biochemical and Biophysical Research Communications, 299, (2), 208-215. (doi:10.1016/S0006-291X(02)02561-5).
Rose, Felicity R.A.J. and Oreffo, Richard O.C. (2002) Bone tissue engineering: hope vs hype. Biochemical and Biophysical Research Communications, 292, (1), 1-7. (doi:10.1006/bbrc.2002.6519).
Rose, Felicity R. A. J. and Oreffo, Richard O.C. (2002) Breakthrough and views, bone tissue engineering: hope vs hype. Biochemical and Biophysical Research Communications, 292, (1), 1-7. (doi:10.1006/bbrc.2002.6519).
Partridge, Kris, Yang, Xuebin, Clarke, Nicholas M.P., Okubo, Yasunori, Bessho, Kazuhisa, Sebald, Walter, Howdle, Steven M., Shakesheff, Kevin M. and Oreffo, Richard O.C. (2002) Adenoviral BMP-2 gene transfer in mesenchymal stem cells: in vitro and in vivo bone formation on biodegradable polymer scaffolds. Biochemical and Biophysical Research Communications, 292, (1), 144-152. (doi:10.1006/bbrc.2002.6623).
Yang, X.B., Roach, H.I., Clarke, N.M.P., Howdle, S.M., Quirk, R., Shakesheff, K.M. and Oreffo, R.O.C. (2001) Human osteoprogenitor growth and differentiation on synthetic biodegradable structures after surface modification. Bone, 29, (6), 523-531. (doi:10.1016/S8756-3282(01)00617-2).
Triffitt, J.T., Oreffo, R.O.C., Virdi, A.S. and Xia, Z. (2001) Osteogenic stem-cell characterization and development: potentials for cytotherapy. Cytotherapy, 3, (5), 413-416. (doi:10.1080/146532401753277760).

Book Section

Tare, Rahul S., Mitchell, Peter D., Kanczler, Janos M. and Oreffo, Richard O.C. (2012) Isolation, differentiation and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo. In, Helfrich, Miep H. H. and Ralston, Stuart H. H. (eds.) Bone Research Protocols. New York, US, Springer , 83-99. (Methods in Molecular Biology, 816). (doi:10.1007/978-1-61779-415-5_7).
Sengers, B.G., Please, C.P., Taylor, M. and Oreffo, R.O.C. (2009) Experimental-computational evaluation of human bone marrow stromal cell migration on trabecular bone structures. In, Bartolo, P.J., Fernandes, P.R., Ruben, R.B., Folgado, J., Almeida, H., Alves, N. and Mendes, A. (eds.) Proceedings of ICTE2009 International Conference on Tissue Engineering. International Conference on Tissue Engineering (ICTE2009) Lisbon, Portugal, IST Press, 119-122. (ECCOMAS thematic conference).

Conference or Workshop Item

Bolland, B.J.R.F., New, A.M.R., Oreffo, R.O.C. and Dunlop, D.G. (2007) The role of vibration in impaction bone grafting. In, Engineers and Surgeons: Joined at the Hip, London, UK, 19 - 21 Apr 2007. UK,
Bolland, B.J.R.F., New, A.M.R., Oreffo, R.O.C. and Dunlop, D.G. (2007) Augmentation of biological and mechanical properties of allograft in impaction bone grafting. In, Engineers and Surgeons: Joined at the Hip, London, UK, 19 - 21 Apr 2007. UK,
Bolland, B.J.R.F., New, A.M.R., Oreffo, R.O.C. and Dunlop, D.G. (2007) The use of vibration-assisted graft compaction in impaction bone grafting: improvement in bone graft strength without increased risk of fracture. At 1st Joint Meeting of the Bone Research Society and the British Orthopaedic Research Society, Southampton, UK, 05 - 06 Jul 2006. UK,
Bolland, B.J.R.F., Partridge, K., New, A.M.R., Dunlop, D.G. and Oreffo, R.O.C. (2007) Augmentation of allograft with human bone marrow stromal cells: validation of cell survival, proliferation, osteogenic phenotype and mechanical strength. In, 1st Joint Meeting of the Bone Research Society and the British Orthopaedic Research Society, Southampton, UK, 05 - 06 Jul 2006. UK,
Bolland, B.J.R.F., New, A.M.R., Oreffo, R.O.C. and Dunlop, D.G. (2007) The role of vibration in impaction bone grafting: reducing the risk of fracture and improving bone graft strength. In, Meeting of the Orthopaedics Section of the Royal Society of Medicine, 01 May 2007. UK,
Bolland, B.J.R.F., New, A.M.R., Oreffo, R.O.C. and Dunlop, D.G. (2007) Improving both the biological and mechanical properties of allograft in impaction bone grafting: a role for human bone marrow stromal cells. In, 8th European Fedaration of National Associations of Orthopaedics and Traumatology Congress, Florence, Italy, 11 - 15 May 2007. Italy,
Bolland, B.J.R.F., New, A.M.R., Oreffo, R.O.C. and Dunlop, D.G. (2007) Vibration assisted femoral impaction bone grafting: reducing the risk of fracture whilst improving bone graft strength. In, British Hip Society: Annual Meeting, Leeds, UK, 28 Feb - 02 Mar 2007. UK,
Bolland, B.J.R.F., New, A.M.R., Oreffo, R.O.C. and Dunlop, D.G. (2007) The use of vibration-assisted graft compaction in impaction bone grafting. Reducing the risk of fracture whilst improving bone graft strength. At 8th European Federation of National Associations of Orthopaedics and Traumatology Congress, Florence, Italy, 11 - 15 May 2007. Italy,
Lanham, S., Roberts, C., Perry, M.J., Cooper, C. and Oreffo, R.O.C. (2006) Intrauterine programming of skeletal development: a longitudinal study. At Bone Research Society Annual Meeting 2006, Southampton, UK, 05 - 06 Jul 2006.
Roach, H.I., Yamanda, N., Hashimoto, K., Cheung, K.S., Oreffo, R.O., Kokubun, S. and Bronner, F. (2006) Epigenetics of osteoarthritis: is DNA Dmethylation involved in the pathogenesis of the disease? At Bone Research Society and British Orthopaedic Research Society 1st Joint Meeting, Southampton, UK, 05 - 06 Jul 2006.
 

Research

Research Interests

The aim of the group is to understand the mechanisms of bone development, growth and regeneration, how these differ in skeletal abnormalities and diseases such as osteoporosis and osteoarthritis, and to what extent bone growth is programmed during fetal life.

Thus research in Professor Oreffo’s group is primarily centered on harnessing the potential of skeletal stem cells (whether derived from embryonic or fetal and adult sources) for the development of unique tissue engineering approaches for new cartilage and bone formation for orthopedic application. In parallel we are interested in the role of fetal programming as a consequence of maternal nutritional challenges on bone cell differentiation, activity, potential and bone function with age.

Skeletal Research - the potential of skeletal stem cells

The requirement for new bone to replace or restore the function of traumatised or degenerated bone, or for the replacement of lost mineralised tissue as a consequence of increasing age is a major clinical and socio-economic need. To date, bone formation stimulation regimes, although attractive, have yet to demonstrate clinical efficacy.

Research of the group has primarily centred on:

Understanding human skeletal stem cell biology and the development of unique tissue engineering approaches for cartilage and bone formation for orthopaedic application using human skeletal populations and, elucidating the role of fetal programming as a consequence of maternal nutritional challenge on mesenchymal progenitor cell differentiation, activity, potential and bone function with age.

This involves drawing together the elements of i) progenitor cell differentiation (specifically the control of mesenchymal stem/progenitor cell differentiation and plasticity), ii) generation of osteoconductive and inductive smart scaffold/materials (including the use of natural biomimetic environments and self -assembling scaffolds with appropriate extracellular matrix cues) and, iii) cell signal/growth factor biology to examine tissue regeneration.

Other areas of active research interest within the group include differentiation of pluripotent stem cells along the mesenchymal lineage as well as angiogenesis / revascularisation in tissue development.

The group is currently developing translational strategies for clinical application to couple cell technologies with biomimetic scaffolds in close collaboration with groups in the UK as well as international collaborations in Germany, Canada and the USA.

Thus current concepts, approaches and challenges from work in the group include:

i) The use of isolated and selected human osteoprogenitor cell populations with selected osteotropic agents in an attempt to modulate the phenotype of the skeletal stem cell to generate mineralised bone tissue,
ii) The role of epigenetics, maternal nutrition and intrauterine programming in skeletal development of related offspring
iii) Manipulation of the developmental potential of bone stem cells on modified biomimetic structures with select growth factors,
iv) Epidemiological Studies in Bone & Joint Research
v) Modelling of skeletal tissue formation – including gene network analysis as well as tissue growth modelling.
vi) Translation from bench to clinic

Although clinical efficacy has yet to be achieved, development of protocols, new tools and above all multidisciplinary approaches for de novo bone formation that utilise skeletal stem cells offer significant rewards for an increasing aged population both in terms of healthcare costs and, more importantly, improved quality of life.

Intrauterine Programming, Epigenetics, Skeletal Development and Bone Diseases

In collaboration with Prof. Cyrus Cooper (MRC Environmental Epidemiology Unit) members of HDH and a number of international collaborators we are investigating, using a variety of animal models, how maternal protein deficiency affects bone growth. Our current ex vivo analysis of bone populations shows an important role of maternal nutrition in subsequent fetal bone development with significant implications in understanding the subsequent development of bone chronic diseases in later life such as osteoarthritis.

Academic unit:  Human Development and Health

Research projects

Application of ultrasound standing wave fields for augmentation of cartilage bioengineering strategies

Targeting stem cells with nanoparticles

Promoting tissue regeneration by carrying drugs and molecules directly to stem cells.

The effects of substrate mechanics on keratinocytes and epidermal stem cell behaviour at wound sites

How does the stiffness of a wound affect how it heals?

The healing of a skin wound is a highly co-ordinated series of events involving both biochemical and biomechanical signalling. We are trying to understanding how the mechanical properties of a healing wound affects how it heals.

Skeletal stem cells

Growth, analysis and characterization (a-g)

Skeletal stem cells

Making Bone

Growth of skeletal stem cells (a) and their expression of a marker enzyme

Making Bone

Skeletal Stem Cells

Growing on allograft bone for clinical application

Skeletal Stem Cells

Bone Development

Slice of trabecular bone captured using Computer Tomography

Bone Development

Responsibilities

Postgraduate Supervision (higher research degrees)

2002 Rahul Tare PhD
2002 Xuebin Yang PhD
2003 Daniel Howard PhD
2006 Michael O’Connell PhD
2006 Sayed-Hadi Mirmalek-Sani PhD
2006 Jodie Pound PhD
2007 Jon Dawson PhD
2008 Simon Tiley MD
2008 Ben Bolland MD
2009 Ahmed El Serafi PhD
2010 Catherine Forristal PhD
2010 Ayshe Ismail PhD
2011 Peter Mitchell PhD
2011 Alex Aarvold MD

Current

14 PhD and MD students (Oct 2011)

PhD

Stephanie Harding ( Year 3)
Kelvin Cheung (Year 3)
Marco Peca ( Year 2)

Siwei Li ( Year 1 – co-supervisor)
Emma McMorrow
Patrick Stumpf (co-supervisor)
Joanna Greenhough (co-supervisor)
Nunzia Sposito (co-supervisor)
Tsiloon Li (co-supervisor)


MD

Andrew Jones
James Smith
Ed Tayton
Emma Williams
Nick Evans

Faculty of Medicine

Associate Dean International and Enterprise

University of Southampton

Southampton Asset Management
Various University Enterprise/Research Groups

National and International responsibilities

Editorial Board Biomaterials
Editorial Board Tissue Engineering
Editorial Board European Cells and Materials
Editorial Board Regenerative Medicine
Editorial Board J. Tissue Engineering
Editorial Board J, Tissue Engineering and Regenerative Medicine

Fellow Institute of Biology
Research Council for Health of the Academy of Finland
Member of BBSRC Healthy Organism Panel (Deputy Chair)
ARC Research Committee
UK National Stem Cell Network Committee

Adjunct Professor King Saud University

Consultancy

Serve and have served as a consultant to various SME and Pharma Companies in the area of musculoskeletal science and Bone Regeneration

Teaching Responsibilities

BM5 Year 4. Delivery of on-going lectures to groups of undergraduate students studying Orthopaedics and Rheumatology. Specific lectures given on metabolic bone disease and basic bone biology.

BM5 Year 1. Provides tutorial and lectures on calcium metabolism and physiology of bone

BM4 Year 1. Provides tutorial to year 1 BM4 students on Bone Physiology.

Integrated Stem Cell PhD Programme – Stem Cell PhD Course Director and  Lead - Also teach and run projects within the programme

Contact

Professor Richard Oreffo
Faculty of Medicine
University of Southampton
Institute of Developmental Sciences Building
Mailpoint 887
Southampton General Hospital
Tremona Road
Southampton SO16 6YD
Tel: +44 (0)23 8120 8502

Room Number: SGH/IDS/MP887