Embryo environment and developmental potential and health
We are interested in how the environment of the oocyte and preimplantation embryo can influence its development and future long-term potential. We investigate how maternal diet (in vivo), maternal sickness (in vivo) or IVF-related culture conditions (in vitro) can affect blastocyst development including gene expression patterns, cell proliferation and cellular phenotype. We derive embryonic stem cell lines to further characterise environmental effects on developmental potential.
We also examine long-term consequences of rodent preimplantation environment on subsequent fetal growth and gene expression, maternal nutrient provision, postnatal growth, cardiovascular and metabolic physiology, behaviour, immune reactivity.
Our data indicate that programming of postnatal phenotype can derive from early embryonic environment which has important implications for embryo potential and fetal/postnatal health. Our studies comprise a range of molecular, epigenetic, cellular and physiological technologies on extra-embryonic and embryonic cell lineages and postnatal tissues.
Mechanisms of early mammalian development
We are also interested in the basic mechanisms regulating early mammalian development. Following fertilization, the egg undergoes a series of cell divisions to form a blastocyst which implants into the uterus wall. The blastocyst initially contains two types of cells, one an outer epithelium (trophectoderm) which gives rise to most of the placental tissues of the conceptus, the other, (inner cell mass), which gives rise to the entire foetus after implantation. Blastocyst formation is therefore an essential first step in our development. We use the mouse embryo to tackle the fundamental questions: How do different cell types expressing different genes and proteins emerge during development? What role is played by cell-cell interactions in this process? How do cells mature and differentiate into an epithelium, the commonest tissue in our bodies?
We utilize mainly animal models for our research (principally the mouse) but also include human embryos where appropriate and under HFEA license.
We have strong links with the School of Medicine at Southampton, particularly staff within DOHaD (Developmental Origins of Health and Disease) and the early human development and stem cell consortium within the university.
Affiliate research group
Joanne Gould (Co SV, Medicine)
Oliver Hutton (Co SV, Medicine)
Pooja Khurana (Main SV, Marie-Curie ESR, EU)
Yi-Lung Chang (Co SV, Overseas)
Anan Aljahdali (Main SV, Overseas)
Completed since 2008:
Ili Raja Khalif (Main SV, Overseas)
Rose Panton (MRC, Main supervisor)
Charlotte Williams (BBSRC, Main SV)
Franchesca Lock (Co SV, Medicine)
Sarah Finn (Co SV, Medicine)
Andy Cox (Co SV, BBSRC)
Congshun Sun (Main SV, University + Private)
Ayat Bakheet (Co SV, Overseas)
Affiliate research group(s)
Institute for Life Sciences (IfLS),
Maternal nutrient restriction exclusive to the preimplantation period has a pronounced influence on fetal and postnatal growth and organ development, as well as postnatal physiology.
Discovering the maternal mechanisms induced by diet which act through embryo developmental plasticity to alter later health.
An analysis of effects of maternal high fat diet on embryo developmental potential.
Mechanisms by which assisted conception treatments may affect embryo development and health into adulthood.
Mechanisms by which maternal diet affects embryo development and health into adulthood.
Effects of assisted conception treatments on embryo development and health into adulthood.
Effect of mouse maternal diet on development and characteristics of neural stem cells.
Professor Tom P. Fleming
Professor of Developmental Biology
University of Southampton
Mailpoint 840, Level D Lab & Path Block
Southampton General Hospital
Telephone:(023) 8120 4145