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The University of Southampton
Biomedical Imaging Unit

Advanced Light Microscopy Course

riple labelling experiment performed and imaged during ALMC course
Triple labelling experiment performed and imaged during ALMC course

This 5 day course is intended to train university staff, postgraduate students and biomedical scientists in the understanding and application of advanced techniques in light microscopy (LM) in the biomedical sciences.

Participants will be given theoretical and practical training in a variety of LM imaging techniques (brightfield, polarising, DIC, darkfield, slide scanning, time lapse, fluorescence, confocal) and then be expected to acquire a portfolio of images from a range of microscopes using both pre-prepared specimens and ones that they prepare themselves. In addition, course participants will acquire skills in analysing, processing and presenting still and video LM data, and be aware of the role, importance and relevance of light microscopy in biological research.

The course is accredited by the Institute of Biomedical Sciences (36 points) and limited to 9 students per course to ensure significant hands on time on a variety of instruments.


Aims and Objectives

By the end of the course participants should:


Total: 11 hrs


Total: 24.5 hrs

For more information on course content and timetable, please use the download links below.

Course Dates and Booking

The course runs approximately once every 6 months and is often over subscribed. To register for a place on the next available course please contact Patricia Goggin (, SGH x4815).


Due to the power and flexibility of our confocal systems, we do not expect course participants to be able to set them up for themselves after this course, rather to understand general principles and experimental considerations. Further 1 to 1 training is then available in the form of 1 day confocal masterclasses.

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