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The University of Southampton
Biomedical Imaging Unit

Introduction to 2D Image Processing and Analysis Course

Introduction to 2D Image Processing and Analysis with Fiji / ImageJ
Introduction to 2D Image Processing and Analysis with Fiji / ImageJ

This 1 day, hands on course is intended to train university staff, postgraduate students and biomedical scientists in the understanding and application of basic principles of 2D image processing and analysis for the biomedical sciences using the freeware image analysis program Fiji / ImageJ.

The course is divided into three parts:


Aims and Objectives

Over the course of 1 day, participants will gain a basic understanding of concepts relating to:

Through multiple examples, the course will cover some basic image processing (filtering, thresholding, image subtraction,…) and analysis (manual measurements, ROI manager, semi-automated measurement,…) procedures as well as the use of macro scripts to batch process multiple images.

Practical Exercises

Part 1: Basic operations

Part 2: Basic processing

Part 3: Basic analysis

Part 5: Macro programming

Course Dates and Booking

The course runs on demand once sufficent participants to fill a course have registered (8). To register for a place on the next available course please contact Anton Page (, SGH x4815).

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