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The University of Southampton
Histochemistry Research Unit

Immunohistochemistry

We have over 20 years experience in the use of immunohistochemical techniques. The HRU has a range of validated protocols suitable for frozen, paraffin and GMA resin sections.

We stock a broad range of primary antibodies including those to identify cells, adhesion molecules, epithelial components, assess cell proliferation status and cytokines optimised for the study of inflammatory processes in a variety of tissues.

The HRU also stocks a range secondary antibodies, detection kits and chromagens.

We have optimised double staining using either two primary antibodies or an antibody in combination with a tinctorial stain.

GMA section of nasal polyp double stained for beta tubulin (pink) and CBE-1 (brown) [Yoshisue H et al]

Double staining

GMA section of nasal polyp double stained for beta tubulin (pink) and CBE-1 (brown) [Yoshisue H et al]

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