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The University of Southampton
µ-VIS: Multidisciplinary, Multiscale, Microtomographic Volume Imaging

3D X-ray Histology (XRH)

What is 3D X-ray Histology (XRH)

3D X-ray Histology

X-ray imaging by micro-computed tomography (µCT) is a key technique in the rapidly developing field of 3D microscopy. Until recently, 3D imaging of wax-embedded histological tissues was beyond the reach of μCT. We have shown that microstructural detail can be captured from standard (non-stained) tissue blocks. Being able to "see" the tissue structure in full 3D can overturn misconceptions of disease development.

3D X-ray histology (XRH) is a µCT -based workflow tailored to fit seamlessly into current histology workflows in biomedical and pre-clinical research, as well as clinical histopathology.

3D X-ray histology allows: 

✓ Non-destructive, distortion-free 3D (volume) imaging of conventionally prepared FFPE tissue specimens
✓ Imaging of conventionally prepared formalin-fixed paraffin-embedded tissue
✓ Spatial resolution down to 5 μm
✓ Correlative 2D histology and immunohistochemistry and 3D visualisation
✓ Whole block imaging
✓ A multitude of 2D and 3D visualisation modes similar to the ones used in clinical radiology, including:
  - multi-planar reconstruction (MPR)
  - maximum intensity projections (MIP)
  - interactive volume rendering
  - 'on-the-fly' arbitrary slicing


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