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The University of Southampton
Biological SciencesPostgraduate study

Mr Brandon Coke MSci

Postgraduate research student

Mr Brandon Coke's photo

Brandon Coke is a postgraduate research student within the School of Biological Sciences, University of Southampton.

Brandon is a PhD student which is part of the SoCobio doctoral training partnership and joined in 2020. Prior to joining the programme, he studied at the university of Birmingham as an integrated masters student degree in Biochemistry. During his masters he worked extensively with organoid 3D tissue cultures and conventional 2D-cultures for qPCR, flow cytometry and comet assays. This built upon his skills he developed as an intern for a molecular diagnostic company- Advanced Molecular Diagnostics. Throughout his undergraduate studies and as an intern he developed his programming skills in both R and Python for data analysis and presentation and this has continued in his pHD wherein he is applying his skills in to analyse proteomic and transcriptomic data to uncover novel β-catenin interactions in leukaemia and identify new druggable targets.

2016-2020 MSci Biochemistry, University of Birmingham.

Research interests

My main interest is to apply transcriptomics and proteomics to gain a better insight into cellular process and identify novel responses. During my masters, I used qPCR extensively to model the changes in the transcriptome between 2D and 3D organoid cultures to assess whether the improved cell-cell interactions in the organoid cultures improve their ability to model the effects of genotoxicants. This was achieved by comparing the expression levels of metabolising enzymes and xenobiotic response element binding transcription factors amongst the different cultures. During my masters; I also used flow cytometry to assess if 3D organoid  were more sensitive than 2D cultures based on an amount of phosphorylated biomarker (γ-H2AX) after genotoxicant exposure.  Currently I am analysing proteomic data from two Wnt-responsive leukemic cancer cell lines (leukemic cells that increase the stability of β-catenin when exposed to canonical Wnt ligands) to identify novel β-catenin interactions. This will eventually lead to wet-lab experiments to elucidate the role of the novel β-catenin binding proteins.

I also have keen interest in using R and python for data analysis and presentation. I have used both programmes extensively to model enzyme michelas menton kinectics, create drug responsive curves and statstical analysis of data. Currently, I am using these tools to analyse both proteomic and transcriptomic data to identify novel β-catenin interactions.

Finally I have keen interest in developing and using 3D-cell organoid cultures to enable cells to more closely mimic in vivo responses due to the improvements to cell-cell  interactions, mechanotransdution and paracrine signalling.

Research project: Interrogating the β-catenin interactome for novel modulators of Wnt signalling

Supervisor: Dr Rob M Ewing

Funding Agency: BBRC

Research group

Computational and Systems Biology

Affiliate research groups

Molecular and Cellular Biosciences, Institute for Life Sciences, Morgan Lab - University of Sussex

Mr Brandon Coke
School of Biological Sciences
Faculty of Environmental and Life Sciences
Life Sciences Building 85
University of Southampton
Highfield Campus
Southampton
SO17 1BJ
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