Biomedical research, applications and many clinical tools are underpinned by modern spectroscopic and imaging techniques. These serve as valuable analytical tools for routine monitoring, diagnosis and prognosis as well as aids to therapeutic intervention such as surgery, transplants, and regular treatments. This module will introduce the key physical principles of different techniques used for spectroscopic and imaging measurements. Based on these principles, the emphasis will be on the applications of relevant techniques to biomedical research and clinical practice, which interrogate various properties of materials and provide information ranging from the molecular to the structural level. Thus they also provide information at different length scales from nano (nm) to macro (m). In this context state-of-the-art developments and applications in spectroscopy, microscopy, super-resolution and large-scale (whole body) imaging will be discussed, including biomedical imaging modalities applied in daily clinical practice. The sessions (lectures, discussion groups and workshops) as well as the lab visits within this module will be offered at the University of Southampton and the Southampton General Hospital by basic and clinical researchers across different disciplines, within the Faculties of Natural and Environmental Sciences, Engineering and the Environment, Physical Sciences and Engineering, and Medicine.