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Research project

Saturation transposon mutagenesis of Chlamydia trachomatis

Project overview

The aim of this project was to develop the first, tightly controlled saturation mutagenesis methodology for the Chlamydiae using a methodology termed TraDIS (transposon directed insertion site sequencing). Saturation mutagenesis can be used to differentiate essential from nonessential genes, which can help in the discovery of new drug or vaccine targets. Through optimisation of gene expression, site-directed mutation of the transposase gene and utilisation of long-read nanopore sequencing to identify transposon insertions, we achieved proof of principle for this important new genetic tool.

Staff

Lead researcher

Professor Ian Clarke

Professor of Virology

Research interests

  • Transposon mutagenesis of Chlamydia species
  • Cell culture systems and replicons for human noroviruses
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Other researchers

Dr Colette O'neill

Lecturer in Pharmacology
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Research outputs

Colette E O'Neill, Rachel J. Skilton, Jade Forster, David W. Cleary, Sarah A. Pearson, David J. Lampe, Nicholas R. Thomson & Ian N. Clarke, 2021, Wellcome Open Research , 6
Type: article