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The University of Southampton

BIOL6097 Skills in Biological Optical Imaging

Module Overview

Aims and Objectives

Learning Outcomes

Learning Outcomes

Having successfully completed this module you will be able to:

  • Understand the theoretical concepts, capabilities and limitations of core biological imaging modalities and their associated techniques and applications.
  • Theoretical knowledge and practical proficiency in the fundamental chemistry and handling required to prepare specimens for transmitted light, fluorescence and live imaging applications.
  • Identify and interpret canonical cell biology markers as viewed through a microscope.
  • Gain competency in a range of core biological imaging modalities.
  • Derive quantitative data from biological images.
  • Learn the analytical options available to them to derive quantitative data for statistical analysis of biological images. They will be able to make basic measurements such as area, length and density as well as use colocalisation and image segmentation algorithms.
  • Review and critically analyse current techniques in biological optical imaging that are of relevance to their own research interests and experience.


The course consists of both practical and theoretical components although the principal focus will be on practical components. The theoretical component will consist of a series of lectures and e-learning resources, which will precede their associated practicals. The practical component will comprise of hands-on laboratory practicals in which students will work in groups and workshops in which students will work independently.

Learning and Teaching

Teaching and learning methods

Lectures, workshops, and practicals

Independent Study99
Practical classes and workshops45
Total study time150



MethodPercentage contribution
Coursework 15%
Coursework 15%
Coursework 35%
Individual Oral Presentation 35%

Repeat Information

Repeat type: Internal

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