This 4 day course is intended to train university staff, postgraduate students and biomedical scientists in the understanding and application of transmission electron microscopy (TEM) in the biomedical sciences.
Participants are trained in methods of sample processing, embedding, cutting and staining (using their own samples if available), the principles and operation of the TEM and the presentation of images (including electronic presentation). In addition, participants will acquire the skills to analyse and present data, and be aware of the role, importance and relevance of TEM in biological research.
The course is accredited by the Institute of Biomedical Sciences (27 points) and is limited to 8 places per session to ensure individual attention and equipment access.
Lectures
- health and safety
- choice of fixatives
- cell structure and resolution
- TEM design
- cryosectioning and immunolabelling for TEM
- special techniques
- data presentation
Total time: 6.5 hrs
Practicals
- fixation
- processing and embedding
- glass knife making
- ultamicrotomy
- staining grids
- TEM operation/sample viewing and imaging
- EM image quiz
Total time: 21 hrs
Dates and how to book
This course runs every 6 months and is often over-subscribed. To register for a place on the next available course, please email Patricia Goggin.