Postgraduate research project

Using soil ancient DNA to assess changing biodiversity in Iceland

Funding
Competition funded View fees and funding
Type of degree
Doctor of Philosophy
Entry requirements
2:1 honours degree View full entry requirements
Faculty graduate school
Faculty of Environmental and Life Sciences
Closing date

About the project

Environmental genomics provides a means to explore the biodiversity of present and past ecosystems. This project will use field work in Iceland and laboratory analyses in UK and Sweden to develop innovative methods for examining long-term floral and faunal changes in Iceland using ancient DNA (aDNA) preserved in soil. 

Soil is an enormous repository of genetic material, and the analysis of soil aDNA has huge potential for addressing important ecological questions that are impracticable to study by other means, e.g., the spatial range of vegetation and soil fauna changes in Iceland pre- and post-colonisation. However, a poor understanding of the fate of DNA entering the soil column hampers efforts to use soil aDNA in palaeo-environmental reconstructions.

This project aims to establish a solid scientific basis for the use of ancient DNA (aDNA) in soil for the reconstruction of terrestrial biodiversity, using a combination of experiments and field sampling in Iceland. To address this knowledge gap, the project will establish field experiments to test the persistence and mobility of DNA in the soil environment; (2) study the century-scale response of vegetation and soil fauna to environmental changes (including human settlement) in Iceland using aDNA preserved in the soil; and formulate protocols that will enable stakeholders to infer long-term changes in terrestrial biodiversity. The successful applicant will conduct field sampling in Iceland and laboratory analyses in the ultra-clean ancient DNA lab at the University of Southampton and the a/eDNA lab at Umeå University, Sweden. With soil aDNA, we’ll build a vast of long-term datasets of soil fauna for various ecological questions. The contribution of this project to science and our society is to improve knowledge to safeguard flora and fauna under changes and provide methods that benefit scientists and stakeholders who monitor terrestrial biodiversity.

Supervisory team

The supervisory team includes supervisors from several organisations. Please contact the Lead Supervisor for more information about the team.

Training

The INSPIRE DTP programme provides comprehensive personal and professional development training alongside extensive opportunities for students to expand their multi-disciplinary outlook through interactions with a wide network of academic, research and industrial/policy partners. The student will be registered at the University of Southampton and hosted at the School of Geography and Environmental Science. Specific training will include:

  • Field experiment and soil sampling in Iceland (two field seasons)
  • Applications of tephrochronology
  • Soil geochemical analysis
  • Molecular analysis in ultra-clean ancient DNA labs, including DNA extraction, DNA metabarcoding, probe-capturing, library preparation and DNA sequencing
  • Bioinformatics and statistical techniques for community analysis; coding development for processing large datasets
  • Paper/thesis writing and presentation skills