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The University of Southampton
Biological Sciences

Research project: Mechanistic insight into the regulatory role of the key kinetochore protein Spc105/KNL1 in chromosome segregation

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A large and mostly unstructured protein Spc105/KNL1 is an essential element of the eukaryotic kinetochore. The project addresses a question of how this protein performs its function as a binding platform for multiple regulatory components to control the proper chromosome segregation during mitosis.

In different model organisms, Spc105/KNL1 protein has been shown to interact with many different kinetochore proteins. In human, depending on the cell origin, these interactions may be critically important for the activity of the spindle assembly checkpoint (SAC). In some animals, Spc105/KNL1 is also necessary for the formation of the so called KMN network, a supra-molecular protein complex that directly connects chromosomes to the microtubules of the mitotic spindle. Additionally, it regulates microtubule dynamicity by either direct binding to microtubules or indirectly, via other effectors. How does it carry out all these functions?

Using Drosophila cells as a model, we are trying to understand some aspects of the regulatory networks, in which Spc105/KNL1 plays a crucial, regulatory role. Here we use in vitro and in vivo binding assays, proteomics and microscopy to better understand the role of this extremely important kinetochore component.

Principal Investigator & Supervisors: Dr Marcin Przewloka (95%), Dr Paul Skipp (5%)
Primary Researcher: Evelin Oroszne Szalai (100%)
Funding provider: The Gerald Kerkut Charitable Trust 50% & SoBS 50%

Related research groups

Molecular and Cellular Biosciences
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