Cancer Sciences Microscopy Facility

More Information

About the Cancer Sciences Microscopy Facility

The Cancer Sciences Microscopy facility provides the equipment and expertise to acquire imaging data from simple bright-field microscopy to state-of-the-art super-resolution imaging.

We provide training in sample preparation, image acquisition and analysis, and data reporting and presentation. The techniques covered encompass imaging of cells and tissues using bright-field, fluorescence and single molecule localisation microscopy. We can also offer training in single-particle electron microscopy for the study of individual protein (antibody) molecules.

The facility was set up to provide microscopy support to Cancer Sciences. However, the equipment and training are available to the Faculty of Medicine, the wider University and external users. We provide:

  • Single molecule localisation microscopy (SMLM)
  • Wide-field fluorescence microscopy
  • Bright-field and phase microscopy
  • Training in sample preparation, imaging, and analysis
  • Cryostat

Technical specification

ONI Nanoimager

The Nanoimager is a benchtop single-molecule localisation microscope (SMLM) purchased via a kind donation from the Mark Benevolent and Cancer Immunology Funds.

  • 3D single particle tracking
  • Total internal reflection fluorescence (TIRF)
  • Single-molecule FRET
  • Resolution up to 20nm in xy, 50nm in z
  • Up to 10ms/100Hz with SPT, TIRF, FRET
  • 4 interlaced, 2 simultaneous imaging channels (405, 488, 561 and 647nm)

Olympus CKX41 inverted fluorescence microscope

The CKX41 is an inverted microscope suitable for cell and tissue observation including GFP and immunofluorescence staining.

  • bright-field and wide-field fluorescence imaging in culture dishes and on slides/coverslips
  • DAPI/FITC/TRITC filters
  • x4, x10 LWD phase objectives, x10, x40 Plan achromat objectives

EVOS M5000 imaging system

The EVOS M5000 imaging system is a fully integrated digital inverted benchtop microscope for three-colour fluorescence, transmitted light, and colour imaging.

  • bright-field, phase and fluorescence imaging of cells in tissue culture dishes
  • DAPI/GFP/RFP filters 
  • x4, x10, x20 LWD Phase objectives

Leica CM1860 cryostat

The Leica CM1860 cryostat is used for sectioning frozen tissue.


Contact us

We welcome your enquiries.

Sonya James