About the project
This project investigates how secretory signalling pathways and cell communication influence the emergence of cancer stem cells (CSCs) during early pancreatic cancer development. Using advanced 3D organoid models, multi-omics, and genetic approaches, it aims to identify key pathways, biomarkers, and therapeutic targets for early detection and intervention in pancreatic ductal adenocarcinoma (PDAC).
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers, largely due to its late diagnosis and limited opportunities for early intervention.
This PhD project aims to uncover the signalling and secretory pathways that drive the transition from normal and inflamed pancreatic tissue to cancer stem cells (CSCs), which are thought to play a crucial role in the earliest stages of cancer development.
You will use advanced three-dimensional (3D) organoid systems derived from patient, murine, and human induced pluripotent stem cell (iPSC) models to explore how CSC communicate locally and systemically through secreted proteins.
These studies will integrate multi-omics approaches, including transcriptomics, proteomics, metabolomics, and secretome profiling, along with functional genetic manipulation to identify pathways that drive early malignant transformation.
A key component of the project will involve helping in establishment of a patient-derived organoid biobank spanning the full spectrum of pancreatic disease—from normal and inflamed (pancreatitis) tissue to pre-malignant and late-stage pancreatic cancer. This unique resource will serve as a foundation for future mechanistic and translational research.
The project offers comprehensive training in organoid culture, molecular and cellular biology, bioinformatics, and translational cancer research.
This unique opportunity is a collaboration between the Advanced Cellular Models Group, Centre for Proteomic Research, and the clinical team at Southampton General Hospital specialising in pancreatic biology and cancer.
The outcomes of this research will advance understanding of the earliest cellular events driving pancreatic cancer and reveal potential biomarkers and molecular targets for prevention and early intervention, providing an exciting opportunity to contribute to a field with major translational impact.
Supervisors
As well as Dr Jessica Nelson, Professor Paul Skipp and Professor Claire Clarkin from the University of Southampton, you will also receive supervision from Zaed Hamady at University Hospital Southampton.
