About the project
PCR has transformed DNA research but relies on specialised equipment. This project aims to develop a reversible linkage system forming dynamic oligonucleotide libraries to template DNA replication. The approach will be used to evolve novel ligases and polymerases, ultimately enabling medicinal applications, including targeted therapies for various cancers.
The polymerase chain reaction (PCR) has revolutionised all aspects of research and applications that involve the manipulation of DNA. While the technology has been optimised and is part of standard protocols in many laboratories, it hinges on the availability of specialised equipment, including PCR cyclers or gel electrophoresis. These are usually not an issue for a well-equipped lab, but may not be available in lesser funded places. In particular technologies like SELEX to find aptamers are resource intensive.
As part of the Horizon Europe funded project ON-TRACT, we will develop a system based on reversible internucleosidic linkages that will create a dynamic combinatorial library of oligonucleotides, and evaluate its properties to replicate (via templating) specific DNA sequences. The system will then be tested for its response to specific templates, which can include up to cell receptors.
The selection and amplification process is promising to deliver aptamers without the need for SELEX. This system will then be further used to evolve novel ligases and polymerases for sustainable oligonucleotide synthesis. Applications in cancer therapy, particularly aimed at lung cancer, are envisaged during this project.
You'll receive training in all scientific aspects around DNA chemistry, organic synthesis, purification and analysis. As this project is part of a European Doctoral Network, there are several network wide training weeks at different European locations planned, where both scientific as well as transferable skills training will be delivered.
The School of Chemistry and Chemical Engineering is committed to promoting equality, diversity inclusivity as demonstrated by our Athena SWAN award. We welcome all applicants regardless of their gender, ethnicity, disability, sexual orientation or age, and will give full consideration to applicants seeking flexible working patterns and those who have taken a career break. The University has a generous maternity policy, onsite childcare facilities, and offers a range of benefits to help ensure employees’ well-being and work-life balance. The University of Southampton is committed to sustainability and has been awarded the Platinum EcoAward.
In addition to the University of Southampton supervisor, this project has the following external supervisors:
- Professor Annemieke Madder (Ghent university)
- Dr Anna Rydzik (Astrazeneca)
