Biomedical MicroCT facility

About the Biomedical MicroCT facility

Microfocus Computed Tomography (μCT) is a non-destructive imaging technique. It uses X-rays to generate high resolution 3D (volume) datasets from samples. This technique offers spatial resolution down to 6 -8 μm. Able to examine conventional hard, high contrast structures, microCT can also be adapted for imaging softer tissues.

A μCT system consists of a microfocus X-ray source and a high-resolution X-ray detector, enabling imaging at resolutions down to a few micrometers. Depending on the system design, the source and detector can either be mounted on a gantry that rotates around the object or remain stationary while the object is mounted on a rotating stage. The former configuration is typically used in in-vivo systems to accommodate live specimens, while the latter is preferred for ex-vivo imaging, as it offers greater geometric flexibility.

Technical specification

MiLabs 3D/4D optical imaging/microCT system

  • X-ray tomography with a spatial resolution down to ca. 13μm in each lateral dimension with nominal isotropic voxel resolution down to 2.4μm
  • 3D fluorescence, bioluminescence and Cherenkov luminescence tomography in the visual to near-IR range (340nm to 830nm; up to six emission filters and up to 11 excitation filters; C-MOS detector) with a resolution of ca. 3mm
  • dyes are separated using spectral unmixing
  • ability to scan living organisms up to the size of a small rabbit (up to five mice can be scanned at once)
  • correlative imaging to map fluorescence/luminescence images onto X-ray CT images and white light images
     

Nikon Med-X (altpha)

130 kVp X-ray source

Customised XRH XT H 225 ST system

  • Nikon multi-target micro focus X-ray source
    130kVp/130W Mo rotating target (custom); 10μm spot up to 50W
    180kVp/20W Mo transmission targets (custom); 1.5μm spot up to 5W
    225kVp/225W multimetal reflection target; 2.5μm spot up to 7W
  • sample autoloader - up to 14 specimens per rack (custom)
  • optimised for high throughput 3D X-ray histology
  • 2.8x2.8k 16-bit flat panel detector
  • samples up to ~400mm and up to 50kg
  • for more details, visit the μ-VIS X-ray imaging centre
     

The two Nikon systems are managed by and run in synergy with the μ-VIS X-ray Imaging Centre and are part of the 3D X-ray Histology (XRH) facility. The facility offers imaging services to internal and external users.

Please contact Dr Orestis Katsamenis (O.Katsamenis@soton.ac.uk) for further information.

Contact us

Contact us

Biomedical Imaging Unit Laboratory and Pathology Block Southampton General Hospital Tremona Road Southampton SO16 6YD
(Open in Google maps)
We’re open Monday to Friday 09:00 to 17:00 UK time.