Project overview
We propose that miRNAs play a critical role in the regulation of skeletal stem cell fate and function. We will determine the role of miRNAs in the maintenance of stem phenotype or enhancement of bone stem cell differentiation and mechanisms involved including cytoskeletal or epigenetic changes. We have recently shown that defined nanotopographies are capable of robustly regulating SSC fate in the absence of chemical cues. We will therefore use custom designed nanotopographies to control SSC phenotype (for instance, to induce osteogenesis or maintain SSC numbers) and examine how miRNAs affect stem cell responses through modulation of epigenetic, biochemical and biomechanical processes. This study will facilitate our understanding of the mechanisms involved in the regulation of skeletal stem cell fate and function.
Research outputs
Ines Moreno,
Signe Gry Hulsart Billstrom,
& Stefanie Inglis
, 2017
, Tissue Engineering Part C Methods
, 23 (12) , 938--952
Type: article
Emma Budd,
Shona Waddell,
Maria C. De Andres,
, 2017
, Current molecular biology reports
, 3 (4) , 263--275
Type: article
Louisa C.Y. Lee,
Nikolaj Gadegaard,
María C. de Andrés,
Lesley-Anne Turner,
Karl V. Burgess,
Stephen J. Yarwood,
Julia Wells,
Manuel Salmeron-Sanchez,
Dominic Meek,
& Matthew J. Dalby
, 2017
, Biomaterials
, 116 , 10--20
Type: article
Glyn N. Stacey,
Che J. Connon,
Karen Coopman,
Alan J. Dickson,
Barry Fuller,
Charles J. Hunt,
Paul Kemp,
Julie Kerby,
Jennifer Man,
Paul Matejtschuk,
Harry Moore,
John Morris,
Nigel Slater,
Stephen Ward,
Claire Wiggins,
& Heiko Zimmermann
, 2017
, Regenerative Medicine
, 12 (5) , 553--564
Type: article
Emma Budd,
Maria De Andres Gonzalez,
, 2017
, Scientific Reports
, 7
DOI: 10.1038/srep46704
Type: article