The University of Southampton

CHEM1040 Introduction to Analytical Chemistry

Module Overview

Analytical Chemistry is a measurement science consisting of a set of powerful ideas and methods that provide qualitative or quantitative information about the chemical composition of a sample. Analytical measurements are required in a wide range of fields beyond the chemical industry such as biochemistry and the pharmaceutical industry, enivronmental sciences, forensic sciences, and the food industry amongst others. The module will provide an introduction into the fundamentals of chemical analysis, including an understanding of some of the most important analytical techniques today (titration and gravimetric analysis, spectroscopic methods including UV-visible, fluorescence, Raman, IR and atomic absorption, chromatography, mass spectrometry, and electroanalysis).

Aims and Objectives

Module Aims

The module aims to equip the students with the basic knowledge of the fundamentals of the theoretical basis behind many techniques in analytical chemistry and their use in range of applications, as well as the statistical treatment of data and the limitations of the various techniques.

Learning Outcomes

Learning Outcomes

Having successfully completed this module you will be able to:

  • understand the underlying theoretical basis of analytical techniques including titration and gravimetric analysis, spectroscopic methods including UV-visible, fluorescence, Raman, IR and atomic absorption, chromatography, mass spectrometry, and electroanalysis;
  • be able to select the appropriate analytical methods to evaluate a sample;
  • critcally evaluate data from a variety of analytical chemsitry techniques and apply knowledge of the statistical analysis of data;
  • have develped the skills required to work as a member of a group;
  • be aware of current developments in the field of analytical chemistry.


The basics of Analytical Chemistry (4 lectures and 1 workshop) • Basics of analytical analysis of data: significant figures, mean, median and spread, quantification of errors, standard deviation, Q-test, T-test, linear least squares. • Titration and gravimetric analysis: acid/base and other titrimetric analyses, gravimetric titrations, solubility, the use of chelating agents, and the concept of thermogravimetric analyses. Spectroscopic methods in Analytical Chemistry (8 lectures and 1 workshop) • UV-visible and fluorescence spectroscopy: review of the electronic transitions of molecules and the Jablonski diagrams, Beer-Lambert law, use of absorption and fluorescence, the components of UV-visible and fluorescence spectrometers. • Infrared and Raman spectroscopy: review of the vibrational modes of molecules and the selection rules for IR and Raman, components of IR and Raman spectrometers, sample preparation, choice of methods. • Atomic spectroscopy: review of the electronic transitions of atoms, components of spectrometers, flame absorption and emission spectrometry, inductively coupled plasma emission spectrometry. Separation techniques and mass spectrometry (4 lectures and 1 workshop) • Separation and chromatography: solvent extractions, solid-phase extraction, calculation of Rf, TLC, GC, and LC, factors affecting chromatographic separation. • Mass spectrometry and labeling methods: the concept of the m/z ratio, components of mass spectrometers, ionisation methods, detection methods, coupled techniques. Electroanalytical and bio-analytical chemistry (6 lectures and 1 workshop) • Electroanalysis: reference electrodes and the components of electrochemical cells, potentiometric methods, pH measurement, ion selective electrodes. • Bio-analytical Chemistry: sample collection and separation, radio-immunological and fluorescence assays, biosensors, glucose sensors.

Learning and Teaching

Teaching and learning methods

Teaching methods: Lectures, directed reading, Seminars, Bb online support. Learning methods: Independent study, group work, student motivated peer group study, student driven tutor support

Follow-up work24
Preparation for scheduled sessions24
Completion of assessment task52
Practical classes and workshops8
Total study time150

Resources & Reading list

D.A. Skoog, D.M. West. Fundamentals of Analytical Chemistry. 

Seamus P.J. Higson (2006). Analytical Chemistry. 





MethodPercentage contribution
Coursework 34%
Group presentation 33%
Group report 33%


MethodPercentage contribution
Coursework 100%
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